Thermal lens spectrophotometry with argon laser excitation source for nitrogen dioxide determination

1983 ◽  
Vol 55 (12) ◽  
pp. 1907-1910 ◽  
Author(s):  
Tatsuji. Higashi ◽  
Totaro. Imasaka ◽  
Nobuhiko. Ishibashi
1984 ◽  
Vol 56 (14) ◽  
pp. 3006-3006 ◽  
Author(s):  
Tatsuji. Higashi ◽  
Totaro. Imasaka ◽  
Nobuhiko. Ishibashi

2002 ◽  
Vol 20 (2-4) ◽  
pp. 99-110 ◽  
Author(s):  
Achamma Kurian ◽  
Nibu A. George ◽  
Binoy Paul ◽  
V. P. N. Nampoori ◽  
C. P. G. Vallabhan

In this paper we report the use of the dual beam thermal lens technique as a quantitative method to determine absolute fluorescence quantum efficiency and concentration quenching of fluorescence emission from rhodamine 6G doped Poly(methyl methacrylate) (PMMA), prepared with different concentrations of the dye. A comparison of the present data with that reported in the literature indicates that the observed variation of fluorescence quantum yield with respect to the dye concentration follows a similar profile as in the earlier reported observations on rhodamine 6G in solution. The photodegradation of the dye molecules under cw laser excitation is also studied using the present method.


2015 ◽  
Vol 46 (8) ◽  
pp. 716-721 ◽  
Author(s):  
Helena Diez-y-Riega ◽  
David Camejo ◽  
Carlos E. Manzanares

1997 ◽  
Vol 3 (S2) ◽  
pp. 25-26
Author(s):  
Hsing-Wen Wang ◽  
Joseph Willis ◽  
Michael V. Sivak ◽  
Joseph A. Izatt

Laser-induced fluorescence (LIF) spectroscopy of tissues has been proposed as an adjunct to endoscopy for in vivo gastrointestinal (GI) diagnosis of premalignant lesions. In previous studies, under ultraviolet excitation (350-370 nm), sources of LIF signal differences in normal, and premalignant colonie tissues have been identified including 1) changes in gross tissue morphology, 2) increased hemoglobin absorption in adenomatous tissues, and 3) increased red fluorescence in dysplastic cells. However, many questions remain regarding the specific origins and biochemical correlates of GI tissue autofluorescence.We report on a study of quantitative ultraviolet LIF spectral microscopy in human colonic tissues using a laser scanning confocal microscope with argon laser excitation 351-364 nm. Frozen sections (6μm) of normal colon (n=10) and tubular adenoma (n=8) were prepared from fresh surgical resection specimens using a previously published protocol. To identify histological components accurately, LSCAM slides were alcohol fixed and H&E histologically stained following confocal imaging.


1999 ◽  
Vol 390 (1-3) ◽  
pp. 163-173 ◽  
Author(s):  
J. Amador-Hernández ◽  
J.M. Fernández-Romero ◽  
G. Ramis-Ramos ◽  
M.D. Luque de Castro

2014 ◽  
Vol 936 ◽  
pp. 2007-2010
Author(s):  
Saksit Sukprasong ◽  
Tanabat Promjun ◽  
Komsanti Chokethawai ◽  
Athipong Ngamjarurojana

The experiment was conducted to study the effects of concentration, temperature and solvent on the luminescence decay curves obtained in laser-induced fluorescence (LIF) experiment from fluorescent orange dye. Sample solutions of fluorescence dye were prepared by mixing with ethanol solvents at different concentration. The sample was illuminated with a radiation of 470 nm using a blue laser excitation source. Luminescence spectra were recorded using a compact fiber coupled CCD spectrometer and the results were analyzed.The results showed that both concentration and temperature affected the luminescence decay of orange dye in the same way. The luminescence decay was decreased when the concentration and temperature were increased.The appearance of peak positions was slighly shifted to higher wavelength (lower photon energy) when concentration were varied. Then the results of concentration effect were analyzed using peak fit, the appearance of peak positions was slightly shifted from 601.51 to 604.12, 607.64, and 618.44 nm at the concentration of 50, 60, 70 and 80 % by volume, respectively.


2005 ◽  
Vol 59 (12) ◽  
pp. 1470-1479 ◽  
Author(s):  
Mikhail A. Proskurnin ◽  
Svetlana N. Bendrysheva ◽  
Nataliya Ragozina ◽  
Stefan Heissler ◽  
Werner Faubel ◽  
...  

The optical scheme of a near-field dual-beam mode-mismatched thermal-lens detector for capillary electrophoresis with a crossed-beam configuration employing a multimode HeCd laser (325 nm) as an excitation source was optimized. It is shown that a multimode laser can be successfully used as an excitation source in thermal lensing with minimal deviations in thermal responses from Gaussian excitation sources. An equation for diffraction thermal-lens theory for near-field measurements is deduced, and the experimental results agree with the deduced equation. The temperature rise in the capillary was estimated, and the exponential decrease of the signal with time for static conditions and low flow velocities was explained. The optimum configuration of the detector from the viewpoint of the maximum sensitivity and beam sizes was found. The detector provides a significant improvement in the detection limits for model compounds absorbing at 325 nm (nitrophenols) compared to the results obtained with a commercial absorbance detector operating at the same wavelength.


1993 ◽  
Author(s):  
Gregory A. Theriault ◽  
Ricardo Newbery ◽  
John M. Andrews ◽  
Sabine E. Apitz ◽  
Stephen H. Lieberman

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