Galactose oxidase enzyme electrode with internal solution potential control

1982 ◽  
Vol 54 (8) ◽  
pp. 1394-1399 ◽  
Author(s):  
Jay M. Johnson ◽  
H. Brian. Halsall ◽  
William R. Heineman
Keyword(s):  
Enzyme Electrode ◽  
Galactose Oxidase ◽  
Internal Solution ◽  
Potential Control ◽  
Oxidase Enzyme

Potentiometric studies at the glucose oxidase enzyme electrode

1994 ◽  
Vol 34 (2) ◽  
pp. 195-198 ◽  
Author(s):  
Sangita D. Kumar ◽  
A.V. Kulkarni ◽  
R.G. Dhaneshwar ◽  
S.F. D'Souza
Keyword(s):  
Glucose Oxidase ◽  
Enzyme Electrode ◽  
Potentiometric Studies ◽  
Oxidase Enzyme

Model for an Immobilized Oxidase Enzyme Electrode in the Presence of Two Oxidants

1994 ◽  
Vol 66 (17) ◽  
pp. 2763-2770 ◽  
Author(s):  
Nicolas. Martens ◽  
Elizabeth A. H. Hall
Keyword(s):  
Enzyme Electrode ◽  
Oxidase Enzyme ◽  
Two Oxidants

scholarly journals A novel lactose biosensor based on electrochemically synthesized 3,4-ethylenedioxythiophene/thiophene (EDOT/Th) copolymer

2020 ◽  
Vol 18 (1) ◽  
pp. 974-985
Author(s):  
Songul Sen Gursoy ◽  
Abdulkerim Yildiz ◽  
Gamze Celik Cogal ◽  
Oguz Gursoy
Keyword(s):  
Limit Of Detection ◽  
Enzyme Concentration ◽  
Electrode System ◽  
Enzyme Electrode ◽  
Galactose Oxidase ◽  
Carbon Electrode ◽  
Working Electrode ◽  
Optimization Parameters ◽  
Cross Linker ◽  
Scanning Electron

AbstractIn this study, a new lactose biosensor has been developed in which the 3,4-ethylenedioxythiophene/thiophene (EDOT/Th) copolymer is used as a transducer. The EDOT/Th copolymer was deposited on the glassy carbon electrode to be used as the working electrode. In addition to the working electrode, the three-electrode system was used in both the electrochemical synthesis and in the biosensor measurements. Lactase (β-galactosidase) that catalyzes the breakdown of lactose into monosaccharides (glucose and galactose) and galactose oxidase that catalyzes the oxidation of the resulting galactose were attached to the copolymer by a cross-linker on the modified working electrode. The response of the enzyme electrode to lactose was determined by cyclic voltammetry (CV) at +0.12 V. Enzyme electrode optimization parameters (pH, temperature, enzyme concentration, etc.) were performed. Fourier transform infrared spectroscopy, scanning electron microscopy and CV methods were used to support copolymer formation. In addition, the characteristics of the enzyme electrode prepared in this study (Km, 0.02 mM; activation energy Ea, 38 kJ/mol; linear working range, up to 1.72 mM; limit of detection, 1.9 × 10−5 M and effects of interferents [uric acid and ascorbic acid]) were determined.

scholarly journals Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

2016 ◽  
Vol 52 (36) ◽  
pp. 6158-6161 ◽  
Author(s):  
Martin J. Weissenborn ◽  
Sandra Notonier ◽  
Sarah-Luise Lang ◽  
Konrad B. Otte ◽  
Susanne Herter ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Microtiter Plate ◽  
Galactose Oxidase ◽  
Screening Assay ◽  
Whole Cell ◽  
Oxidase Enzyme

A whole cell screening assay for terminal hydroxylases was developed based on a readily available galactose oxidase enzyme.

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