Thin-layer chromatography with in situ multichannel image detection of fluorescent compounds

1981 ◽  
Vol 53 (9) ◽  
pp. 1357-1361 ◽  
Author(s):  
M. L. Gianelli ◽  
J. B. Callis ◽  
G. D. Christian ◽  
N. H. Andersen ◽  
G. D. Christian
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Image Detection ◽  
Layer Chromatography ◽  
Fluorescent Compounds

Quantitative in situ thin-layer chromatography of ergocalciferol in multivitamin tablets

1978 ◽  
Vol 11 (4) ◽  
pp. 217-219 ◽  
Author(s):  
M. Širec ◽  
Lj. Mikša ◽  
T. Bičan-Fišter ◽  
M. Prošek ◽  
E. Kučan
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

Microchannel Thin Layer Chromatography with in situ Plate Scanning Micro-DRIFTS Detection Using Plain and Polybutadiene Modified Zirconia Stationary Phases

2002 ◽  
Vol 56 (8) ◽  
pp. 1059-1066 ◽  
Author(s):  
Beata A. Musial ◽  
André J. Sommer ◽  
Neil D. Danielson
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Diffuse Reflectance ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Diffuse Reflectance Infrared ◽  
Layer Chromatography ◽  
Tlc Separation ◽  
Zirconia Stationary Phases

Thin layer chromatography (TLC) of various dyes is compared in microchannels packed with either bare zirconia (normal phase) or polybutadiene (PBD) modified zirconia (reversed phase). In situ micro-diffuse reflectance infrared Fourier transform spectroscopy (μDRIFTS) of the analyte spots is possible due to the low absorption background of both plain and PBD zirconia. An instrument coupling μDRIFTS with a motorized stage that could profile the microchannel TLC plate is developed. The retention order of anthracene and pyrene probes as a function of percent acetonitrile are generally as expected on PBD zirconia but opposite for plain zirconia. Impurities in technical grade methylene blue can be determined after TLC separation on plain zirconia. The separation and infrared identification of various dyes such as dichlorofluorescein and rhodamine B on PBD zirconia is improved using a mobile phase with an additive such as phosphate or dihexylamine that prevents band streaking.

Detection of in situ Compound Instability by Two-Dimensional Thin-layer Chromatography

1966 ◽  
Vol 55 (4) ◽  
pp. 429-430 ◽  
Author(s):  
Melvin H. Penner ◽  
Joseph M. Talmage ◽  
Milton Geller
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Two Dimensional ◽  
Layer Chromatography

SEPARATION AND CHARACTERISATION OF Δ4-3-KETOSTEROIDS OF THE PREGNANE-SERIES BY MEANS OF THIN-LAYER CHROMATOGRAPHY

1963 ◽  
Vol 43 (1) ◽  
pp. 47-66 ◽  
Author(s):  
B. P. Lisboa
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Thinlayer Chromatography ◽  
Pregnane Series ◽  
Layer Chromatography

ABSTRACT A method is described for the separation and characterisation of thirty-two Δ4-3-ketosteroids of the pregnane-series. The method involves thinlayer chromatography on Silica Gel G of the various steroids and of various derivatives thereof, followed by seven in situ colour reactions. Data are presented on the reproducibility of the method.

Determination of Matacil and Zectran by Fluorigenic Labeling, Thin Layer Chromatography, and In Situ Fluorimetry

1972 ◽  
Vol 55 (6) ◽  
pp. 1259-1264
Author(s):  
R W Frei ◽  
J F Lawrence
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Sulfonyl Chloride ◽  
Relative Standard ◽  
Dansyl Derivatives ◽  
Layer Chromatography ◽  
Derivatives Of ◽  
Fluorescent Derivatives

Abstract The fluorigenic labeling of Matacil (4-dimethylamino- m-tolyl N-methylcarbamate) and Zectran (4-dimethylamino-3,5-xylyl Wmethylcarbamate) with dansyl chloride (1-dimethylamino- naphthalene-5-sulfonyl chloride) results in 3 fluorescent derivatives, and labeling with NBD-Cl (4-chloro-7-nitrobenzo- 2,1,3-oxadiazole) produces 2 fluorescent derivatives for each carbamate, all of which can be separated by thin layer chromatography (TLC). These derivatives are identified by nuclear magnetic resonance, infrared, and fluorescence spectroscopy, aided by TLC data. The carbamates are hydrolyzed in dilute base and the resulting amine or phenol hydrolysis products react with the labeling reagents. The derivatives are analyzed by TLC and in situ fluorimetry with a spectrophotometer in the fluorescence mode and a spectrophotofluorometer with the thin layer scanning accessory. Reactions, fluorescence phenomena, and chromatographic properties of the derivatives are investigated for evaluation of the method as a quantitative technique. A reproducibility of 3–5% relative standard deviation can be expected in the concentration range from 15 to 300 ng/spot for derivatives of the 2 labeling procedures. The dansyl derivatives are instrumentally detectable as low as 1 ng/spot while the NBD derivatives may be detected at concentrations of less than 0.5 mg/spot.

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