Effect of ultrasound on the separation of DNA fragments in agarose gel electrophoresis

1990 ◽  
Vol 62 (11) ◽  
pp. 1194-1196 ◽  
Author(s):  
Yinfa. Ma ◽  
Edward S. Yeung
Keyword(s):  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Dna Fragments ◽  
Agarose Gel Electrophoresis

scholarly journals Agarose Gel Electrophoresis for the Separation of DNA Fragments

10.3791/3923 ◽  
2012 ◽  
Author(s):  
Pei Yun Lee ◽  
John Costumbrado ◽  
Chih-Yuan Hsu ◽  
Yong Hoon Kim
Keyword(s):  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Dna Fragments ◽  
Agarose Gel Electrophoresis

DNA Breaks Induction by Mimosine

2003 ◽  
Vol 58 (9-10) ◽  
pp. 732-735 ◽  
Author(s):  
Emil Mladenov ◽  
Boyka Anachkova
Keyword(s):  
Nuclear Matrix ◽  
Gel Electrophoresis ◽  
Average Length ◽  
Agarose Gel ◽  
Dna Fragments ◽  
Agarose Gel Electrophoresis ◽  
Chromosomal Dna ◽  
Dna Breaks ◽  
Random Size ◽  
Random Fashion

AbstractMouse erythroleukemic F4 N cells were treated with mimosine, etoposide, Fe(II)-EDTA, and Cu(II) in the presence of ascorbate. DNA was isolated and subjected to agarose gel electrophoresis and the size and distribution of the DNA fragments produced by the agents were compared. With increasing concentration of Cu(II) the production of DNA fragments was increased without decrease of the average length of the fragments, and their sizes were similar to those produced by etoposide as expected for cleavage of DNA at the nuclear matrix attachments sites. In contrast, mimosine and Fe(II) produced fragments of random size and with the progression of the reaction the average length of the fragments decreased. These results indicate that mimosine cuts DNA in a random fashion, regardless of its higher order chromatin organization. A conclusion is drawn that the DNA fragments obtained after mimosine treatment are a result of mimosine-assisted, Fe(II) dependent Fenton-like reactions randomly cutting chromosomal DNA.

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

1988 ◽  
Vol 60 (02) ◽  
pp. 133-136 ◽  
Author(s):  
R Schneppenheim ◽  
H Plendl ◽  
U Budde
Keyword(s):  
Von Willebrand Factor ◽  
Gel Electrophoresis ◽  
Detection Methods ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Luminescence Assay ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

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