Flow system for direct determination of enzyme substrate in undiluted whole blood

1990 ◽  
Vol 62 (9) ◽  
pp. 932-936 ◽  
Author(s):  
Thomas. Buch-Rasmussen
Keyword(s):  
Whole Blood ◽  
Flow System ◽  
Direct Determination ◽  
Enzyme Substrate

scholarly journals Studies on Agranulocytosis. III. The Reduced Glutathione (GSH) Content of Leukocytes of Normals and Patients Recovered from Agranulocytosis

Blood ◽  
1960 ◽  
Vol 16 (5) ◽  
pp. 1572-1578 ◽  
Author(s):  
ANTHONY V. PISCIOTTA ◽  
MARY DALY
Keyword(s):  
Whole Blood ◽  
Reduced Glutathione ◽  
Mental Disease ◽  
Complete Solution ◽  
Direct Determination

Abstract 1. A method for the direct determination of GSH in leukocytes is described. Treatment with alkali (0.5 M. NaOH) effects complete solution of the white cells and after deproteinization (5 per cent HPO3), the GSH is determined by the sensitive "alloxan 305" procedure. Control studies showed that under the conditions of the test, GSH is not affected by the alkali and no splitting of soluble minus SH from protein occurs. 2. Using this method, the amount of reduced glutathione content of normal leukocytes was found to be 5.2 ± 1 mg, per 1010 WBC. 3. No differences from normal levels were detected for the leukocytic GSH of patients with mental disease and those susceptible to agranulocytosis. Incubation of whole blood with the drug which caused agranulocytosis had no effect upon the GSH content of leukocytes.

Colorimetric determination of potassium in whole blood, serum, and plasma.

1985 ◽  
Vol 31 (9) ◽  
pp. 1464-1467 ◽  
Author(s):  
S T Wong ◽  
J Spoo ◽  
K C Kerst ◽  
T G Spring
Keyword(s):  
Whole Blood ◽  
Colorimetric Method ◽  
Direct Determination ◽  
Ion Pair ◽  
Photometric Method ◽  
Colorimetric Determination ◽  
Two Dimensional ◽  
Subsequent Formation ◽  
Blood Specimens

Abstract This spectrophotometric method for the direct determination of potassium in serum or plasma is based on the selective complexing of potassium by a specific macrocyclic polyether, with the subsequent formation of an ion-pair with a colored anion. The colored anion is extracted into an organic solvent, clarified by centrifugation, and then measured at 415 nm. The absorbance of the chromogen varies linearly with [K+] to at least 15 mmol/L. Results of this colorimetric method (y) correlate well with the results obtained by a flame-photometric method (y = 1.04x - 0.22, r = 0.97, n = 81), with CVs ranging from 2 to 4%. We observed no interferences from lipemia, added bilirubin, or various electrolytes. We also evaluated the use of this reagent in a new automated blood analyzer developed by Abbott, a two-dimensional centrifugal system (Clin Chem 31:1457-1463, 1985). Potassium determined with this system (y) correlated well with results by flame photometry: y = 1.02x + 0.02 (r = 0.94, n = 168). With this system one can use whole-blood specimens in measuring potassium.

Direct determination of five fluoroquinolones in chicken whole blood and in veterinary drugs by HPLC

2005 ◽  
Vol 28 (4) ◽  
pp. 325-331 ◽  
Author(s):  
Victoria F. Samanidou ◽  
Eleni A. Christodoulou ◽  
Ioannis N. Papadoyannis
Keyword(s):  
Whole Blood ◽  
Direct Determination ◽  
Veterinary Drugs

Direct Determination of Cadmium and Lead in Whole Blood by Potentiometric Stripping Analysis

1992 ◽  
Vol 38 (10) ◽  
pp. 1995-2001 ◽  
Author(s):  
P Ostapczuk
Keyword(s):  
Detection Limit ◽  
Blood Sample ◽  
Whole Blood ◽  
Deposition Time ◽  
Supporting Electrolyte ◽  
Direct Determination ◽  
Stripping Analysis ◽  
Lead And Cadmium ◽  
Potentiometric Stripping Analysis

Abstract The commercially available equipment for potentiometric stripping analysis (PSA) was tested for routine lead and cadmium determination in whole-blood samples. In contrast to anodic stripping voltammetry, PSA is not subject to background interferences from organic electroactive constituents in the sample or to the presence of dissolved oxygen (i.e., oxygen removal is not necessary). To determine lead and cadmium by PSA, it is sufficient to dilute the blood sample with an appropriate supporting electrolyte (0.5 mol/L HCl). The detection limit changes with deposition time and volume of blood sample used. For 1 mL of blood and a 1-min deposition time, the detection limit is 1 microgram/L for both elements. If the deposition time increases to 10 min, cadmium can be determined at its normal concentration in blood (the detection limit is improved to < 0.1 microgram/L). Procedures for routine determination of lead and cadmium in whole blood are presented.

Direct determination of selenium in whole blood by anodic stripping voltammetry

1997 ◽  
Vol 127 (1-2) ◽  
pp. 67-70 ◽  
Author(s):  
Mehmet Sayim Karacan ◽  
G�ler Somer ◽  
?�kr� Kalayci
Keyword(s):  
Whole Blood ◽  
Anodic Stripping Voltammetry ◽  
Direct Determination ◽  
Stripping Voltammetry ◽  
Anodic Stripping
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