Determination of mono- and oligosaccharides in fermentation broths by liquid chromatographic separation and amperometric detection using immobilized enzyme reactors and a chemically modified electrode

1989 ◽  
Vol 61 (8) ◽  
pp. 831-838 ◽  
Author(s):  
Gyoergy A. Marko-Varga
Keyword(s):  
Immobilized Enzyme ◽  
Amperometric Detection ◽  
Chemically Modified Electrode ◽  
Chemically Modified ◽  
Enzyme Reactors ◽  
Immobilized Enzyme Reactors ◽  
Liquid Chromatographic Separation ◽  
Liquid Chromatographic ◽  
Fermentation Broths

scholarly journals The First Liquid-Chromatographic Separation of the (R)- and (S)-Enantiomers of a Chiral Silanol, Silane and Germane

1995 ◽  
Vol 50 (4) ◽  
pp. 568-572 ◽  
Author(s):  
Reinhold Tacke ◽  
Dirk Reichel ◽  
Kurt Günther ◽  
Stefan Merget
Keyword(s):  
Chiral Stationary Phase ◽  
Enantiomeric Purity ◽  
Nmr Studies ◽  
Enantiomerically Pure ◽  
Chemically Modified ◽  
Liquid Chromatographic Separation ◽  
Racemic Mixtures ◽  
Liquid Chromatographic ◽  
C Nmr

The racemic mixtures of the muscarinic antagonists cyclohexyl(phenyl)(2-pyrrolidinoethyl)silanol (sila-procyclidine, rac-1), cyclohexyl(hydroxymethyl)phenyl(2-piperidinoethyl)-silane (rac-2) and cyclohexyl(hydroxymethyl)phenyl(2-piperidinoethyl)germane (rac-3) were resolved by analytical liquid chromatography (HPLC) using chemically modified cellulose (1) or amylose (2, 3) as the chiral stationary phase. This chromatographic method was used for the quantitative determination of enantiomeric purity of the (R)- and (S)-enantiomers of 1 - 3 , which were obtained by preparative resolution with chiral auxiliary agents. Furthermore, the enantiomeric purity of these samples was established by 13C NMR studies using chiral shift reagents. According to these studies, the resolved antipodes of 1 - 3 (obtained by preparative resolution) were almost enantiomerically pure [HPLC: ≥ 98.2% ee ((R )-3) to 99.4% ee ((R)-1, (S)-1); NMR: ≥ 97% ee].

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