Piezoelectric crystal biosensor modified with protein A for determination of immunoglobulins

1987 ◽  
Vol 59 (23) ◽  
pp. 2760-2763 ◽  
Author(s):  
Hiroshi. Muramatsu ◽  
Jonathan M. Dicks ◽  
Eiichi. Tamiya ◽  
Isao. Karube
Keyword(s):  
Protein A ◽  
Piezoelectric Crystal

EGF receptor signaling induces pointed P1 transcription and inactivates Yan protein in the Drosophila embryonic ventral ectoderm

Development ◽  
1996 ◽  
Vol 122 (11) ◽  
pp. 3355-3362 ◽  
Author(s):  
L. Gabay ◽  
H. Scholz ◽  
M. Golembo ◽  
A. Klaes ◽  
B.Z. Shilo ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Target Genes ◽  
Egf Receptor ◽  
Protein A ◽  
Drosophila Embryo ◽  
Cell Fates ◽  
Secondary Target ◽  
Graded Activity ◽  
Definition Of

The induction of different cell fates along the dorsoventral axis of the Drosophila embryo requires a graded activity of the EGF receptor tyrosine kinase (DER). Here we have identified primary and secondary target genes of DER, which mediate the determination of discrete ventral cell fates. High levels of DER activation in the ventralmost cells trigger expression of the transcription factors encoded by ventral nervous system defective (vnd) and pointed P1 (pntPl). Concomitant with the induction of pntP1, high levels of DER activity lead to inactivation of the Yan protein, a transcriptional repressor of Pointed-target genes. These two antagonizing transcription factors subsequently control the expression of secondary target genes such as otd, argos and tartan. The simultaneous effects of the DER pathway on pntP1 induction and Yan inactivation may contribute to the definition of the border of the ventralmost cell fates.

Computational determination of the pigment binding motif in the chlorosome protein a of green sulfur bacteria

2013 ◽  
Vol 118 (3) ◽  
pp. 231-247 ◽  
Author(s):  
Sándor Á. Kovács ◽  
William P. Bricker ◽  
Dariusz M. Niedzwiedzki ◽  
Peter F. Colletti ◽  
Cynthia S. Lo
Keyword(s):  
Protein A ◽  
Green Sulfur Bacteria ◽  
Binding Motif ◽  
Sulfur Bacteria ◽  
Green Sulfur

Radioimmunoassay for pregnancy-associated plasma protein A.

1982 ◽  
Vol 28 (1) ◽  
pp. 50-53 ◽  
Author(s):  
M J Sinosich ◽  
B Teisner ◽  
J Folkersen ◽  
D M Saunders ◽  
J G Grudzinskas
Keyword(s):  
Detection Limit ◽  
Amniotic Fluid ◽  
Plasma Protein ◽  
Early Pregnancy ◽  
Twin Pregnancy ◽  
Protein A ◽  
Trophoblastic Disease ◽  
Coefficients Of Variation ◽  
Highly Sensitive

Abstract A specific and highly sensitive radioimmunoassay for determination of pregnancy-associated plasma protein A in human serum is described. The minimum detection limit for this protein was 2.9 micrograms/L. The within- and between-assay coefficients of variation were 4.0 and 4.5%, respectively. The circulating protein was detected within 32 days of conception in eight normal pregnancies and within 21 days in a twin pregnancy. Circulating concentrations in the mother at term were consistently higher (10-fold) than in matched amniotic fluid; none was detected in the umbilical circulation. This protein was also detected in the circulation of patients with hydatiform mole. This assay will permit investigations into the clinical evaluation of measurements of the protein during early pregnancy and trophoblastic disease.

Pattern Analysis Meets Cell Biology

1999 ◽  
Vol 5 (S2) ◽  
pp. 510-511
Author(s):  
Robert F. Murphy ◽  
Michael V. Boland
Keyword(s):  
Cell Biology ◽  
Fluorescent Dyes ◽  
Protein A ◽  
Chinese Hamster ◽  
Subcellular Location ◽  
Computer Applications ◽  
Double Labeling ◽  
A Cell ◽  
Widespread Availability

The widespread availability of automated fluorescence microscope systems has led to an explosion in the acquisition of digital images by biologists. This has created a need for computer applications that automate the analysis of these images and an opportunity to develop new approaches to classical problems. An example is the determination of the subcellular location of a protein from immunofluorescence images (or, more recently, images of GFP fluorescence). Current practice is to compare such images to mental images that a cell biologist has developed over time, and to reach a tentative conclusion about the structure (i.e., organelle) that a protein is found in. Since this determination is subjective, it often must be followed up by double labeling with a marker protein from the suspected structure.As an initial exploration of the feasibility of automating the determination of subcellular location, we developed a system that is able to classify the localization patterns characteristic of five cellular molecules (proteins and DNA) in Chinese Hamster Ovary (CHO) cells. Images were acquired on an epifluorescence microscope after the cells had been fixed, permeabilized, and labeled with appropriate fluorescent reagents (usually antibodies conjugated to fluorescent dyes). The labels used were directed against a Golgi protein, a lysosomal protein, a nuclear protein, a cytoskeletal protein, and DNA.

scholarly journals Determination of phenotypes and pneumococcal surface protein A family types of Streptococcus pneumoniae from Malaysian healthy children

2013 ◽  
Vol 46 (3) ◽  
pp. 180-186 ◽  
Author(s):  
Masura Mohd Yatim ◽  
Siti Norbaya Masri ◽  
Mohd Nasir Mohd Desa ◽  
Niazlin Mohd Taib ◽  
Syafinaz Amin Nordin ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Protein A ◽  
Surface Protein ◽  
Healthy Children ◽  
Pneumococcal Surface Protein A ◽  
Family Types

PROGNOSTIC SIGNIFICANCE OF SPECIFIC PROTEINS OF PREGNANCY IN WOMEN WITH A UTERINE AT SCAR AND PLACENTA ACCRETA

2020 ◽  
Vol 65 (6) ◽  
pp. 353-357
Author(s):  
Vladimir Anatolyevich Borovkov ◽  
M. B. Igitova ◽  
Y. V. Korenovskiy ◽  
Yu. A. Dudareva
Keyword(s):  
Pregnant Women ◽  
Placenta Accreta ◽  
Screening Program ◽  
Protein A ◽  
Prognostic Significance ◽  
Pregnancy Proteins ◽  
Diagnostic Thresholds ◽  
Diagnostic Threshold ◽  
Specific Proteins

Comparative analysis of serum concentrations of chorionic gonadotropin (hCG) associated with the pregnancy of plasma protein A (PAPP-A) and alpha-fetoprotein (AFP), based on the results of a survey of women as part of a standard screening program (the results were expressed as a MoM - multiply of the median), found a significant increase in the performance of all the studied specific pregnancy proteins in women with a scar on the uterus and placenta acctera (75 patients) compared with the data of the group of pregnant women without scar on the uterus and without abnormalities of attachment of the placenta (150 women). AFP indices were 1.68 ± 0.76 and 1.19 ± 0.43 MoM (p = 0.0018), hCG - 1.62 ± 1.48 and 1.23 ± 0.76 MoM (p = 0, 0112), PAPP-A - 1.93 ± 1.24 and 1.23 ± 0.67 MoM (p <0.0001). Using the ROC analysis, the diagnostic thresholds for the concentrations of AFP, hCG and PAPP-A were calculated. The risk of placenta accreta in women with a scar on the uterus in cases of exceeding the diagnostic threshold of AFP concentration (1.64 MoM) increased 2.5 times (RR = 2.5; 95% CI 1.17-5.36, p = 0, 0185), hCG (1.41 MoM) - 1.6 times (RR = 1.59; 95% CI 1.09-2.32, p = 0.0147), PAPP-A (1.41 MoM) - 2.65 times (RR = 2.65; 95% CI 1.76-3.99, p <0.0001). Determination of the level of specific pregnancy proteins can be used in the system of complex prediction of placental growth in pregnant women with a scar on the uterus as an addition to the assessment of clinical and anamnestic risk factors.

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