Theoretical description of nonlinear chromatography, with applications to physicochemical measurements in affinity chromatography and implications for preparative-scale separations

1987 ◽  
Vol 59 (9) ◽  
pp. 1286-1295 ◽  
Author(s):  
James L. Wade ◽  
Alan F. Bergold ◽  
Peter W. Carr
Keyword(s):  
Affinity Chromatography ◽  
Theoretical Description ◽  
Preparative Scale ◽  
Preparative Scale Separations ◽  
Nonlinear Chromatography ◽  
Physicochemical Measurements

Carrier-aided centrifugal partition chromatography for preparative-scale separations

The Analyst ◽  
1985 ◽  
Vol 110 (8) ◽  
pp. 913 ◽  
Author(s):  
Takeo Araki ◽  
Yasuo Kubo ◽  
Tetsuya Toda ◽  
Masao Takata ◽  
Tetsushi Yamashita ◽  
...  
Keyword(s):  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Preparative Scale ◽  
Preparative Scale Separations

The microstructure of functionalized poLyacrylonitrile beads for bioseparations

1990 ◽  
Vol 48 (4) ◽  
pp. 1094-1095
Author(s):  
Eduardo A. Kamenetzky ◽  
David A. Ley
Keyword(s):  
Aqueous Solution ◽  
Affinity Chromatography ◽  
Pore Size Distribution ◽  
Pore Size ◽  
Surface Coverage ◽  
Vacuum Impregnation ◽  
Thin Sections ◽  
Selective Staining ◽  
Low Viscosity ◽  
Diamond Knife

The microstructure of polyacrylonitrile (PAN) beads for affinity chromatography bioseparations was studied by TEM of stained ultramicrotomed thin-sections. Microstructural aspects such as overall pore size distribution, the distribution of pores within the beads, and surface coverage of functionalized beads affect performance properties. Stereological methods are used to quantify the internal structure of these chromatographic supports. Details of the process for making the PAN beads are given elsewhere. TEM specimens were obtained by vacuum impregnation with a low-viscosity epoxy and sectioning with a diamond knife. The beads can be observed unstained. However, different surface functionalities can be made evident by selective staining. Amide surface coverage was studied by staining in vapor of a 0.5.% RuO4 aqueous solution for 1 h. RuO4 does not stain PAN but stains, amongst many others, polymers containing an amide moiety.

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