Effect of sample stream radius upon light scatter distributions generated with a Gaussian beam light source in the sheath flow cuvette

1987 ◽  
Vol 59 (6) ◽  
pp. 846-850 ◽  
Author(s):  
Fahimeh. Zarrin ◽  
Norman J. Dovichi
Keyword(s):  
Gaussian Beam ◽  
Light Source ◽  
Light Scatter ◽  
Sheath Flow ◽  
Sample Stream ◽  
Sheath Flow Cuvette

scholarly journals DEVELOPMENT OF OPTICAL TRANSDUCER ON FABRICS FOR THE APPLICATION OF LIQUID CLUSTERING USING REFLECTANCE SPECTROSCOPY

2021 ◽  
Vol 5 (2) ◽  
Author(s):  
Viona Hazar Briliana ◽  
Totok Mujiono
Keyword(s):  
Light Source ◽  
Monochromatic Light ◽  
Reflectance Spectroscopy ◽  
Wearable Device ◽  
Light Scatter ◽  
Optic System ◽  
Pca Method ◽  
The Difference ◽  
Scatter Intensity ◽  
Optical Transducer

Recently, usage of fabrics as wearable device, along with their applications are increasing, one example being the detection of bio-analyzes such as blood or sweat. One method used to observe the properties of the material of a fabric is to use the Refcletance Spectroscopy, in which excitation of monochromatic light with a specific wavelength is given to a fabrics. Intensity value is then processed using the PCA method in order to obtain the pattern of the difference between each substrate. The proposed transducer optic system consists of 405nm blueviolet laser as the light source, biconvex lens, Adafruit AS7262 light detector, and Arduino. This system can only detect the difference in substrate content from the occurring light scatter. This system can be applied to various kinds of fabric wearable material with differing scatter intensity values depending on the kind of fabrics. Softer kind of fabric is proposed as material for the wearable device because it gives a high scatter intensity value and constant values in every repetation which results in better data reading.Keywords: clustering, optical, reflectance, spectroscopy, transducer, wearable.

scholarly journals Multiple labeling using two-color immunofluorescence with only one light source, two fluorescence photomultiplier tubes, and two light scatter detectors

Cytometry ◽  
1994 ◽  
Vol 15 (3) ◽  
pp. 272-276 ◽  
Author(s):  
Issam Mansour ◽  
Mohamed A. Jarraya ◽  
Pierre Gane ◽  
Marie F. Reznikoff
Keyword(s):  
Light Source ◽  
Light Scatter ◽  
Photomultiplier Tubes

Sheath-Flow Cuvette for High-Sensitivity Laser-Induced Fluorescence Detection in Capillary Electrophoresis

2007 ◽  
Vol 61 (7) ◽  
pp. 777-779 ◽  
Author(s):  
Kimia Sobhani ◽  
David A. Michels ◽  
Norman J. Dovichi
Keyword(s):  
Stainless Steel ◽  
Capillary Electrophoresis ◽  
Carbonic Anhydrase ◽  
High Sensitivity ◽  
Laser Induced Fluorescence ◽  
Flow Chamber ◽  
Fluorescence Detector ◽  
Sheath Flow ◽  
Laser Induced Fluorescence Detection ◽  
Sheath Flow Cuvette

The sheath-flow cuvette is a key component in a high-sensitivity post-column laser-induced fluorescence detector for capillary electrophoresis. Most designs are based on commercial cuvettes originally manufactured for use in a flow cytometer. In these devices, a quartz flow chamber is held in a stainless-steel fixture that is difficult to machine and subjects the cuvette to a torque when sealed, which frequently leads to damage of the flow chamber. In this report we present a design for a cuvette that may easily be constructed. This design uses compression to hold and seal the quartz flow chamber without applying torque. The system produces detection limits (3σ) of 115 yoctomoles (70 copies) for FQ-labeled carbonic anhydrase.

Microlens Array Homogenizer for Laser Illuminated Projector

2007 ◽  
Vol 364-366 ◽  
pp. 143-147
Author(s):  
Cheng Huan Chen ◽  
Chien Chuan Chen ◽  
Po Hung Yao
Keyword(s):  
Liquid Crystal ◽  
Laser Beam ◽  
Gaussian Beam ◽  
Uniform Distribution ◽  
Light Source ◽  
Laser Light ◽  
Polarized Light ◽  
Microlens Array ◽  
Diffractive Element ◽  
Laser Light Source

Laser light source is a potential illumination light source for non-emissive display applications, especially for liquid crystal projection displays, due to its requirement of low etendue source and highly polarized light. In order to make a conversion from a circular Gaussian beam profile to a rectangular uniform distribution, a microlens array has been proposed as a homogenizer. The analytical and experimental results show that the microlens array with a pitch of 100um under laser beam illumination works as a diffractive element, and a promising uniformity can be obtained with a stack of two microlens array.

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