Fluorescence detection and identification of nitro derivatives of polynuclear aromatic hydrocarbons by on-column catalytic reduction to aromatic amines

1986 ◽  
Vol 58 (8) ◽  
pp. 1827-1834 ◽  
Author(s):  
Silvestre B. Tejada ◽  
Roy B. Zweidinger ◽  
John E. Sigsby
Keyword(s):  
Fluorescence Detection ◽  
Aromatic Hydrocarbons ◽  
Aromatic Amines ◽  
Catalytic Reduction ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Detection And Identification ◽  
Nitro Derivatives ◽  
Derivatives Of

Multi-alkylated Polynuclear Aromatic Hydrocarbons of Tobacco Smoke: Separation and Identification

1978 ◽  
Vol 9 (4) ◽  
Author(s):  
M. E. Snook ◽  
R. F. Severson ◽  
R. F. Arrendale ◽  
H. C. Higman ◽  
O. T. Chortyk
Keyword(s):  
Liquid Chromatography ◽  
Cigarette Smoke ◽  
Aromatic Hydrocarbons ◽  
Ultra Violet ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Gel Chromatography ◽  
Cigarette Smoke Condensate ◽  
Gas And Liquid Chromatography ◽  
Silicic Acid Chromatography ◽  
Derivatives Of

AbstractThe methyl, multi-methyl, and ethyl derivatives of the polynuclear aromatic hydrocarbons (PAH) of cigarette smoke condensate (CSC) were isolated from the neutrals by silicic acid chromatography, solvent partitioning and gel chromatography. The procedure yielded a relatively pure PAH isolate amenable to further identifications. The multi-alkylated PAH were concentrated in the early gel fractions with parent and higher ring PAH found in subsequent gel fractions. It was shown that CSC is very rich in alkylated PAH, and their successful identification required extensive use of gas and liquid chromatography and ultra-violet and GC - mass spectrometric techniques. High-pressure liquid chromatography (HPLC) separated individual isomers of the alkylated PAH in complex GC peaks. PAH from indene to pentamethylchrysene were found. This report concludes our identification studies on the PAH of CSC and complements our two previous reports in this journal. Collectively, our studies have identified approximately 1000 PAH of cigarette smoke condensate and have led to the development of methods for the routine quantitation of PAH in smalI quantities of cigarette smoke condensate.

Determination of Polynuclear Aromatic Hydrocarbons in Seafood by Liquid Chromatography with Fluorescence Detection

1992 ◽  
Vol 75 (5) ◽  
pp. 872-877 ◽  
Author(s):  
Gracia A Perfetti ◽  
Patricia J Nyman ◽  
Sheryl Fisher ◽  
Frank L Joe ◽  
Gregory W Diachenko
Keyword(s):  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Aromatic Hydrocarbons ◽  
Solid Phase ◽  
Reversed Phase ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Matrix Interferences ◽  
Phase Liquid ◽  
Certified Values

Abstract Modification of a previously published method for determination of polynuclear aromatic hydrocarbons (PAHs) produces very clean seafood extracts in less than half the time. After alkaline digestion of the seafood, PAHs were partitioned into 1,1,2- trichlorotrifluoroethane. The resulting extract was cleaned up by solid-phase extraction on alumina, silica, and C18 adsorbents and then analyzed by gradient reversed-phase liquid chromatography with programmable fluorescence detection. Average recoveries of 12 PAHs [acenaphthene, anthracene, fluoranthene, pyrene, benz(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)- fluoranthene, benzo(a)pyrene, dibenz(a,h)anthracene, benzo(ghi)perylene, and indeno(1,2,3-cd)pyrene] from 5 different matrixes (mussels, oysters, clams, crabmeat, and salmon) spiked at low partsper- billion levels ranged from 76 to 94%. Estimated limits of quantitation ranged from 0.01 to 0.6 ppb PAHs in extracts that were free of matrix interferences. Results of analyses of a mussels standard reference material obtained from the National Institute of Standards and Technology were in good agreement with the certified values.

Sign in / Sign up

Export Citation Format

Share Document