Phase Discrimination in Continuous Liquid-Liquid Extraction with Diode Array Photometric Detection

1994 ◽  
Vol 66 (17) ◽  
pp. 2794-2798 ◽  
Author(s):  
F. Ortiz-Boyer ◽  
J. A. Garcia-Mesa ◽  
M. D. Luque de Castro
Keyword(s):  
Liquid Extraction ◽  
Diode Array ◽  
Photometric Detection ◽  
Phase Discrimination ◽  
Liquid Liquid Extraction

Study of the film formation process in liquid-liquid extraction flow injection analysis

1991 ◽  
Vol 56 (6) ◽  
pp. 1228-1237 ◽  
Author(s):  
Vlastimil Kubáň
Keyword(s):  
Flow System ◽  
Detection System ◽  
Film Formation ◽  
Liquid Extraction ◽  
Photometric Detection ◽  
Liquid Liquid Extraction ◽  
Teflon Tube ◽  
Continuous Stream ◽  
Computer Controlled ◽  
Segmented Volume

The behaviour of a thin film of an organic solvent on the walls of the extraction coil in a continuous liquid-liquid extraction flow system was studied using a computer-controlled fast-recording on-tube photometric detection system (approx. 3 ms time resolution). A single-loop injector was employed to introduce precise, reproducible volumes (Sr < 2%) of one phase into the continuous stream of the other as a segmented volume standard. The film thickness Df, ranging from 1 to 20 μm for a 0.7 mm teflon tube, was calculated from the segment lengthening at a different chloroform flow rates and was found to obey a polynominal dependence on the linear flow rate, df = f(uα), where α < 1.

scholarly journals Liquid-liquid extraction combined with high performance liquid chromatography-diode array-ultra-violet for simultaneous determination of antineoplastic drugs in plasma

2011 ◽  
Vol 47 (2) ◽  
pp. 363-371 ◽  
Author(s):  
Ananda Lima Sanson ◽  
Suéllen Cristina Rennó Silva ◽  
Matheus Coutinho Gonçalves Martins ◽  
Alexandre Giusti-Paiva ◽  
Patrícia Penido Maia ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Liquid Extraction ◽  
Therapeutic Drug ◽  
Diode Array ◽  
Antineoplastic Drugs ◽  
Liquid Liquid Extraction ◽  
Relative Standard

A liquid-liquid extraction (LLE) combined with high-performance liquid chromatography-diode array detection method for simultaneous analysis of four chemically and structurally different antineoplastic drugs (cyclophosphamide, doxorubicin, 5-fluorouracil and ifosfamide) was developed. The assay was performed by isocratic elution, with a C18 column (5 µm, 250 x 4.6 mm) and mobile phase constituted by water pH 4.0- acetonitrile-methanol (68:19:13, v/v/v), which allowed satisfactory separation of the compounds of interest. LLE, with ethyl acetate, was used for sample clean-up with recoveries ranging from 60 to 98%. The linear ranges were from 0.5 to 100 µg mL-1, for doxorubicin and 1 to 100 µg mL-1, for the other compounds. The relative standard deviations ranged from 5.5 to 17.7%. This method is a fast and simple alternative that can be used, simultaneously, for the determination of the four drugs in plasma, with a range enabling quantification of the drugs in pharmacokinetics, bioequivalence and therapeutic drug-monitoring studies.

scholarly journals Bioequivalence study of FlunacTM and DiflucanTM in healthy Bangladeshi male volunteers

KYAMC Journal ◽  
2013 ◽  
Vol 2 (2) ◽  
pp. 159-163 ◽  
Author(s):  
UK Sarker ◽  
M Misbahuddin ◽  
MA Hossain
Keyword(s):  
High Performance ◽  
Area Under The Curve ◽  
Bioequivalence Study ◽  
Liquid Extraction ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Time Intervals ◽  
Liquid Liquid Extraction ◽  
Single Cross

A bioequivalence study of a local antifungal drug, fluconazole (FlunacTM), was compared with that of innovator product DiflucanTM. The study was conducted on 15 healthy volunteers and single cross-over dose of 150 mg fluconazole was administered orally. Two milliliter of blood was collected at different time intervals for 96 hours. The drug was extracted by liquid-liquid extraction and estimated by high performance liquid chromatography with diode array detector (260 nm). The chromatographic separation was accomplished using C18 analytical column with a mobile phase consisting of water and acetonitril (80:20, v/v). The Cmax of DiflucanTM and FlunacTM was 2.48 ± 0.29 g/mL and 2.23 ± 0.29 g/mL respectively. The Tmax of DiflucanTM and FlunacTM was 2.18 ± 0.98 hours and 2.56 ± 0.81 hours respectively. The area under the curve (0 to 96 hours) of DiflucanTM and FlunacTM was 73.28 ± 13.80 hours/mL and 74.49 ± 16.03 hours/mL. The half-life of both the drugs was 44.59 ± 13.79 hours for DiflucanTM and 42.73 ± 11.71 hour for FlunacTM. This study shows that FlunacTM is comparable to DiflucanTM in pharmacokinetic aspect.DOI: http://dx.doi.org/10.3329/kyamcj.v2i2.13255KYAMC Journal Vol.2(2) January 2012, 159-163

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