"Wired" Enzyme Electrodes for Amperometric Determination of Glucose or Lactate in the Presence of Interfering Substances

1994 ◽  
Vol 66 (15) ◽  
pp. 2451-2457 ◽  
Author(s):  
Timothy J. Ohara ◽  
Ravi. Rajagopalan ◽  
Adam. Heller
Keyword(s):  
Amperometric Determination ◽  
Enzyme Electrodes ◽  
Wired Enzyme

scholarly journals Fully Inkjet-Printed Biosensors Fabricated with a Highly Stable Ink Based on Carbon Nanotubes and Enzyme-Functionalized Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1645
Author(s):  
Mijal Mass ◽  
Lionel S. Veiga ◽  
Octavio Garate ◽  
Gloria Longinotti ◽  
Ana Moya ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Hydrogen Peroxide ◽  
Catalytic Activity ◽  
Stability Loss ◽  
Amperometric Determination ◽  
Redox Species ◽  
Enzyme Electrodes ◽  
Operational Conditions ◽  
Walled Carbon Nanotubes

Enzyme inks can be inkjet printed to fabricate enzymatic biosensors. However, inks containing enzymes present a low shelf life because enzymes in suspension rapidly lose their catalytic activity. Other major problems of printing these inks are the non-specific adsorption of enzymes onto the chamber walls and stability loss during printing as a result of thermal and/or mechanical stress. It is well known that the catalytic activity can be preserved for significantly longer periods of time and to harsher operational conditions when enzymes are immobilized onto adequate surfaces. Therefore, in this work, horseradish peroxidase was covalently immobilized onto silica nanoparticles. Then, the nanoparticles were mixed into an aqueous ink containing single walled carbon nanotubes. Electrodes printed with this specially formulated ink were characterized, and enzyme electrodes were printed. To test the performance of the enzyme electrodes, a complete amperometric hydrogen peroxide biosensor was fabricated by inkjet printing. The electrochemical response of the printed electrodes was evaluated by cyclic voltammetry in solutions containing redox species, such as hexacyanoferrate (III/II) ions or hydroquinone. The response of the enzyme electrodes was studied for the amperometric determination of hydrogen peroxide. Three months after the ink preparation, the printed enzyme electrodes were found to still exhibit similar sensitivity, demonstrating that catalytic activity is preserved in the proposed ink. Thus, enzyme electrodes can be successfully printed employing highly stable formulation using nanoparticles as carriers.

Gold Nanoparticles Modified Titania Nanotube Arrays for Amperometric Determination of Ascorbic Acid

2010 ◽  
Vol 43 (18) ◽  
pp. 2809-2822 ◽  
Author(s):  
T. G. Satheesh Babu ◽  
P. V. Suneesh ◽  
T. Ramachandran ◽  
Bipin Nair
Keyword(s):  
Gold Nanoparticles ◽  
Ascorbic Acid ◽  
Nanotube Arrays ◽  
Amperometric Determination ◽  
Titania Nanotube Arrays ◽  
Titania Nanotube

Simultaneous determination of lactose and glucose in milk using two working enzyme electrodes

Talanta ◽  
1994 ◽  
Vol 41 (6) ◽  
pp. 843-848 ◽  
Author(s):  
Takashi Katsu ◽  
Xianen Zhang ◽  
Garry A. Rechnitz
Keyword(s):  
Simultaneous Determination ◽  
Enzyme Electrodes
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