Scanning electrochemical microscopy. 24. Enzyme ultramicroelectrodes for the measurement of hydrogen peroxide at surfaces

1993 ◽  
Vol 65 (24) ◽  
pp. 3605-3614 ◽  
Author(s):  
Benjamin R. Horrocks ◽  
David. Schmidtke ◽  
Adam. Heller ◽  
Allen J. Bard
Keyword(s):  
Hydrogen Peroxide ◽  
Scanning Electrochemical Microscopy ◽  
Electrochemical Microscopy

scholarly journals Ultramicrosensors based on transition metal hexacyanoferrates for scanning electrochemical microscopy

2013 ◽  
Vol 4 ◽  
pp. 649-654 ◽  
Author(s):  
Maria A Komkova ◽  
Angelika Holzinger ◽  
Andreas Hartmann ◽  
Alexei R Khokhlov ◽  
Christine Kranz ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Detection Limit ◽  
Transition Metal ◽  
Prussian Blue ◽  
Electrochemical Deposition ◽  
Mixed Layer ◽  
Scanning Electrochemical Microscopy ◽  
The Stability ◽  
Mixed Layers ◽  
Electrochemical Microscopy

We report here a way for improving the stability of ultramicroelectrodes (UME) based on hexacyanoferrate-modified metals for the detection of hydrogen peroxide. The most stable sensors were obtained by electrochemical deposition of six layers of hexacyanoferrates (HCF), more specifically, an alternating pattern of three layers of Prussian Blue and three layers of Ni–HCF. The microelectrodes modified with mixed layers were continuously monitored in 1 mM hydrogen peroxide and proved to be stable for more than 5 h under these conditions. The mixed layer microelectrodes exhibited a stability which is five times as high as the stability of conventional Prussian Blue-modified UMEs. The sensitivity of the mixed layer sensor was 0.32 A·M−1·cm−2, and the detection limit was 10 µM. The mixed layer-based UMEs were used as sensors in scanning electrochemical microscopy (SECM) experiments for imaging of hydrogen peroxide evolution.

Redox reactions of reactive oxygen species in aqueous solutions as the probe for scanning electrochemical microscopy of single live T24 cells

2010 ◽  
Vol 88 (6) ◽  
pp. 569-576 ◽  
Author(s):  
Xiaocui Zhao ◽  
Mengni Zhang ◽  
Yitao Long ◽  
Zhifeng Ding
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Aqueous Solutions ◽  
Redox Reactions ◽  
Reactive Oxygen ◽  
Scanning Electrochemical Microscopy ◽  
Electrochemical Technique ◽  
Oxygen Species ◽  
T24 Cells ◽  
Electrochemical Microscopy

The redox reactions of two main components of reactive oxygen species (ROS), superoxide and hydrogen peroxide, along with oxygen in aqueous solutions were investigated using a conventional electrochemical technique, differential pulse voltammetry (DPV). Superoxide undergoes oxidation at a Pt working electrode biased at 0.055 V versus Ag/AgCl, while hydrogen peroxide can be oxidized and reduced at 0.817 and –0.745 V, respectively. Oxygen in the solutions is reduced at the electrode with an applied potential of –0.455 V. Based on these results, hydrogen peroxide and superoxide released from live cells can be successfully monitored, identified, and mapped using scanning electrochemical microscopy (SECM) at different potentials. Single human bladder (T24) cells were imaged using a 5 μm diameter SECM probe biased at –0.400, –0.600, and –0.800 V. Oxygen reduction that seems an interference can be discriminated from that of hydrogen peroxide by means of SECM.

scholarly journals Detection of hydrogen peroxide produced at a liquid/liquid interface using scanning electrochemical microscopy

2009 ◽  
Vol 11 (2) ◽  
pp. 473-476 ◽  
Author(s):  
Fei Li ◽  
Bin Su ◽  
Fernando Cortes Salazar ◽  
Raheleh Partovi Nia ◽  
Hubert H. Girault
Keyword(s):  
Hydrogen Peroxide ◽  
Scanning Electrochemical Microscopy ◽  
Liquid Interface ◽  
Electrochemical Microscopy

High-Throughput Screening of Hydrogen Peroxide (Electro)Catalysts Using Scanning Electrochemical Microscopy (SECM)

2020 ◽  
Vol MA2020-01 (46) ◽  
pp. 2657-2657
Author(s):  
Yuanya Zhao ◽  
Matthew Kromer ◽  
Jason Adams ◽  
David Flaherty ◽  
Joaquin Rodriguez-Lopez
Keyword(s):  
Hydrogen Peroxide ◽  
High Throughput ◽  
High Throughput Screening ◽  
Scanning Electrochemical Microscopy ◽  
Electrochemical Microscopy

Prussian Blue-modified ultramicroelectrodes for mapping hydrogen peroxide in scanning electrochemical microscopy (SECM)

2012 ◽  
Vol 23 ◽  
pp. 102-105 ◽  
Author(s):  
Oleg G. Voronin ◽  
Andreas Hartmann ◽  
Charlotte Steinbach ◽  
Arkady A. Karyakin ◽  
Alexei R. Khokhlov ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Prussian Blue ◽  
Scanning Electrochemical Microscopy ◽  
Electrochemical Microscopy
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