Separation and Detection of Intact Noncovalent Protein Complexes from Mixtures by On-Line Capillary Isoelectric Focusing-Mass Spectrometry

2000 ◽  
Vol 72 (21) ◽  
pp. 5356-5360 ◽  
Author(s):  
Suzana Martinović ◽  
Scott J. Berger ◽  
Ljiljana Paša-Tolić ◽  
Richard D. Smith
Keyword(s):  
Mass Spectrometry ◽  
Isoelectric Focusing ◽  
Protein Complexes ◽  
Capillary Isoelectric Focusing ◽  
Noncovalent Protein ◽  
On Line

On-line capillary isoelectric focusing–mass spectrometry for quantitative analysis of peptides and proteins

2005 ◽  
Vol 37 (3) ◽  
pp. 423-428 ◽  
Author(s):  
Yukihiro Kuroda ◽  
Hideo Yukinaga ◽  
Masahiro Kitano ◽  
Taeko Noguchi ◽  
Mahboob Nemati ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Isoelectric Focusing ◽  
Capillary Isoelectric Focusing ◽  
On Line

On-line capillary isoelectric focusing hyphenated to native electrospray ionization mass spectrometry for the characterization of interferon-γ and variants

The Analyst ◽  
2015 ◽  
Vol 140 (2) ◽  
pp. 543-550 ◽  
Author(s):  
Cédric Przybylski ◽  
Meriem Mokaddem ◽  
Mehdi Prull-Janssen ◽  
Els Saesen ◽  
Hugues Lortat-Jacob ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Isoelectric Focusing ◽  
Electrospray Ionization Mass Spectrometry ◽  
Interferon Γ ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Capillary Isoelectric Focusing ◽  
Detection Mode ◽  
On Line

The on-line hyphenation of Capillary IsoElectric Focusing (CIEF) with ElectroSpray Ionization Mass Spectrometry (ESI/MS) has been carried out in a non-denaturing detection mode at the CIEF-MS interface.

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