Accuracy in the Determination of Isoelectric Points of Some Proteins and a Peptide by Capillary Isoelectric Focusing:  Utility of Synthetic Peptides as Isoelectric Point Markers

2000 ◽  
Vol 72 (19) ◽  
pp. 4747-4757 ◽  
Author(s):  
Kiyohito Shimura ◽  
Wang Zhi ◽  
Hiroyuki Matsumoto ◽  
Ken-ichi Kasai
Keyword(s):  
Isoelectric Focusing ◽  
Isoelectric Point ◽  
Synthetic Peptides ◽  
Capillary Isoelectric Focusing ◽  
Isoelectric Points

Isoelectric Point Determination of Live Polioviruses by Capillary Isoelectric Focusing with Whole Column Imaging Detection

2013 ◽  
Vol 85 (12) ◽  
pp. 6089-6094 ◽  
Author(s):  
Yvonne E. Thomassen ◽  
Gerco van Eikenhorst ◽  
Leo A. van der Pol ◽  
Wilfried A. M. Bakker
Keyword(s):  
Isoelectric Focusing ◽  
Isoelectric Point ◽  
Capillary Isoelectric Focusing ◽  
Imaging Detection ◽  
Point Determination

A multivariate approach for the determination of isoelectric point of human carbonic anhydrase isoforms by capillary isoelectric focusing

2011 ◽  
Vol 32 (20) ◽  
pp. 2857-2866 ◽  
Author(s):  
Marie Lecoeur ◽  
Jean-François Goossens ◽  
Claude Vaccher ◽  
Jean-Paul Bonte ◽  
Catherine Foulon
Keyword(s):  
Carbonic Anhydrase ◽  
Isoelectric Focusing ◽  
Isoelectric Point ◽  
Capillary Isoelectric Focusing ◽  
Multivariate Approach ◽  
Human Carbonic Anhydrase

scholarly journals Isoelectric points of erabutoxins and monoacyl derivatives of erabutoxin b. Estimation of the pK values of amino groups in erabutoxins by using isoelectric-focusing data

1982 ◽  
Vol 203 (2) ◽  
pp. 427-433 ◽  
Author(s):  
N UI ◽  
C Takasaki ◽  
N Tamiya
Keyword(s):  
Isoelectric Focusing ◽  
Isoelectric Point ◽  
Amino Group ◽  
Amino Groups ◽  
Sea Snake ◽  
Isoelectric Points ◽  
Point Data ◽  
Laticauda Semifasciata ◽  
Erabutoxin B ◽  
Derivatives Of

The isoelectric points of erabutoxins a, b and c, neurotoxic proteins of a sea snake, Laticauda semifasciata, were determined by density-gradient isoelectric focusing. The same measurement was also made with monoacyl derivatives of erabutoxin b, in which each one of all amino groups had been either acetylated or propionylated. Erabutoxins a and b showed the same isoelectric point at pH 9.68. The values for [1-N alpha-acetyl-arginine]-, [15-N6-acetyl-lysine]-, [27-N6-acetyl-lysine]-, [47-N6-propionyl-lysine]- and [51-N6-acetyl-lysine]-erabutoxin b were at pH 9.52, 9.31, 9.45, 9.22 and 9.09 respectively, being definitely different from each other and lower than the value for the unmodified molecule. The isoelectric point of erabutoxin c, which is [51-asparagine]-erabutoxin b, was the same as that of [51-N6-acetyl-lysine]erabutoxin b. Assuming that no change in pK occurs on monoacylation, the pK values of amino groups in erabutoxin b were calculated from the isoelectric-point data. It is indicated that the pK values of zeta-amino groups differ markedly from each other and that the value of alpha-amino group is anomalously high.

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