Utility of Accurate Mass Tags for Proteome-Wide Protein Identification

2000 ◽  
Vol 72 (14) ◽  
pp. 3349-3354 ◽  
Author(s):  
Thomas P. Conrads ◽  
Gordon A. Anderson ◽  
Timothy D. Veenstra ◽  
Ljiljana Paša-Tolić ◽  
Richard D. Smith
Keyword(s):  
Protein Identification ◽  
Accurate Mass

Protein Identification with a Single Accurate Mass of a Cysteine-Containing Peptide and Constrained Database Searching

2000 ◽  
Vol 72 (6) ◽  
pp. 1112-1118 ◽  
Author(s):  
David R. Goodlett ◽  
James E. Bruce ◽  
Gordon A. Anderson ◽  
Beate Rist ◽  
Ljiljana Pasa-Tolic ◽  
...  
Keyword(s):  
Protein Identification ◽  
Accurate Mass ◽  
Database Searching

Theoretical and Experimental Prospects for Protein Identification Based Solely on Accurate Mass Measurement

2004 ◽  
Vol 3 (1) ◽  
pp. 61-67 ◽  
Author(s):  
Fei He ◽  
Mark R. Emmett ◽  
Kristina Håkansson ◽  
Christopher L. Hendrickson ◽  
Alan G. Marshall
Keyword(s):  
Protein Identification ◽  
Mass Measurement ◽  
Accurate Mass Measurement ◽  
Accurate Mass

Analysis of Squalene and Its Transformation By-Products in Latent Fingermarks by Ultrahigh-Pressure Liquid Chromatography-High Resolution Accurate Mass Orbitrap™ Mass Spectrometry

2019 ◽  
Author(s):  
Buddhika Dorakumbura ◽  
Francesco Busetti ◽  
Simon Lewis
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Storage Conditions ◽  
Ultrahigh Pressure ◽  
Accurate Mass ◽  
Rational Approach ◽  
By Products ◽  
Over Time ◽  
High Resolution Accurate Mass

<p>Transformation of squalene and its by-products in fingermarks over time under different storage conditions (light, dark and underwater) was examined through ultrahigh-pressure liquid chromatography high resolution accurate mass Orbitrap™ mass spectrometry. Complications of assessing fingermark compositional variation over time using multiple samples with varying initial compositions were elucidated and a more rational approach was successfully demonstrated. Squalene was detected in all fresh natural fingermarks and the amount ranged between 0.20 to 11.32 μg/5 fingertips. A notable difference in the transformation of squalene was observed with different storage conditions, where a dark aquatic environment accelerated degradation of squalene compared to dark but dry conditions. Squalene monohydroperoxide was extremely short-lived in natural deposits while the amount of squalene epoxide was still increasing relative to the initial amount, after ageing under dark and aquatic conditions for up to 7 days. Some oxidation by-products of cholesterol were also tentatively identified, which exhibited a growth over time against their initial concentration under any of the storage condition tested. These by-products, therefore, show potential as biomarkers for targeted visualisation of aged deposits.</p>

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