Reversed-Phase High-Performance Liquid Chromatography Combined with On-Line UV Diode Array, FT Infrared, and1H Nuclear Magnetic Resonance Spectroscopy and Time-of-Flight Mass Spectrometry:  Application to a Mixture of Nonsteroidal Antiinflammatory Drugs

2000 ◽  
Vol 72 (16) ◽  
pp. 3922-3926 ◽  
Author(s):  
Dave Louden ◽  
Alan Handley ◽  
Steve Taylor ◽  
Eva Lenz ◽  
Steve Miller ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Reversed Phase ◽  
Nonsteroidal Antiinflammatory Drugs ◽  
Resonance Spectroscopy ◽  
Diode Array ◽  
Antiinflammatory Drugs ◽  
Flight Mass Spectrometry ◽  
On Line ◽  
Mass Spectrometry Application

scholarly journals Antioxidant and Antidiabetic Activity of Proanthocyanidins from Fagopyrum dibotrys

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2417
Author(s):  
Xin Li ◽  
Jingling Liu ◽  
Qinxiang Chang ◽  
Ziyun Zhou ◽  
Ruilian Han ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
High Performance ◽  
Grape Seed ◽  
Reversed Phase ◽  
Degree Of Polymerization ◽  
Antidiabetic Activity ◽  
Resonance Spectroscopy ◽  
Flight Mass Spectrometry ◽  
13C Nuclear Magnetic Resonance

Proanthocyanidins are natural glycosidase inhibitors with excellent antioxidant activity. This study aims to search for a new source of proanthocyanidins for the prevention and treatment of type 2 diabetes with higher content and better activity and get their structure elucidated. First, the total proanthocyanidins contents (TOPCs), antioxidant activity, antidiabetic activity of seven common Polygonaceae plants were analyzed and compared. Then proanthocyanidins from the rhizome of Fagopyrum dibotrys were purified, and the detailed structure was comprehensively analyzed by ultraviolet visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), 13C nuclear magnetic resonance spectroscopy (13C NMR), reversed-phase high-performance liquid chromatography-electrospray mass spectrometry (RP-HPLC-ESI-MS), and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). The rhizome of F. dibotrys showed the highest TOPCs, the strongest antioxidant, and antidiabetic activities; the TOPCs, antioxidant and antidiabetic activities were all very significantly positively correlated. Proanthocyanidins purified from the rhizome of F. dibotrys showed better antidiabetic activity than grape seed proanthocyanidins (GsPs). Seventy-two proanthocyanidins from trimer to undecamer with a mean degree of polymerization (mDP) of about 5.02 ± 0.21 were identified with catechin and epicatechin as the dominant monomers. Conclusion: Proanthocyanidins are the main antioxidant and antidiabetic active substances of F. dibotrys and are expected to be developed into potential antioxidant and hypoglycemic products.

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