Analysis of genetic markers in forensic DNA samples using the polymerase chain reaction

1991 ◽  
Vol 63 (1) ◽  
pp. 2-15 ◽  
Author(s):  
Rebecca. Reynolds ◽  
George. Sensabaugh ◽  
Edward. Blake
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Markers ◽  
Forensic Dna ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Synergy between DNA polymerases increases polymerase chain reaction inhibitor tolerance in forensic DNA analysis

2010 ◽  
Vol 405 (2) ◽  
pp. 192-200 ◽  
Author(s):  
Johannes Hedman ◽  
Anders Nordgaard ◽  
Charlotte Dufva ◽  
Birgitta Rasmusson ◽  
Ricky Ansell ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Analysis ◽  
Dna Polymerases ◽  
Forensic Dna ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Inhibitor ◽  
Forensic Dna Analysis ◽  
Inhibitor Tolerance ◽  
Polymerase Chain

Vanadium accelerates polymerase chain reaction and expands the applicability of forensic DNA testing

2013 ◽  
Vol 20 (4) ◽  
pp. 326-333 ◽  
Author(s):  
Junko Kaminiwa ◽  
Katsuya Honda ◽  
Yukiko Sugano ◽  
Shizue Yano ◽  
Takeki Nishi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Testing ◽  
Forensic Dna ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Forensic Dna Testing

scholarly journals DETERMINATION OF MOLECULAR GENETIC MARKERS IN PROGNOSIS OF THE EFFECTIVENESS OF TREATMENT OF MALIGNANT INTRACEREBRAL BRAIN TUMORS

2019 ◽  
Vol 4 ◽  
pp. 25-34
Author(s):  
Oleksandr Glavatskyi ◽  
Irina Vasileva ◽  
Olena Galanta ◽  
Hennadii Khmelnytskyi ◽  
Irina Shuba ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Brain Tumors ◽  
Real Time ◽  
Genetic Markers ◽  
Predictive Value ◽  
Molecular Genetic ◽  
Chain Reaction ◽  
Mgmt Gene ◽  
Polymerase Chain

Intracerebral malignant brain tumors remain one of the most complex problems of neuro-oncology. Today, promising results of the use of targeted drugs have been received, which determine the important diagnostic and predictive value of molecular genetic markers of glial and metastatic brain tumors. Aim: The study of the prevalence of MGMT (O6-methylguanine-DNA methyltransferase) and PTEN (phosphatase and tensin homologue deleted on chromosome 10) gene expression by real time polymerase chain reaction in tumor tissue of gliomas and brain metastases. Materials and methods: From thirty patients were received tumor material (29 cases of glioma III-IV degree of anaplasia and one case of metastatic brain lesion of adenocarcinoma). The normalized expression of MGMT and PTEN genes was determined by real-time polymerase chain reaction. Results: In all 30 (100 %) patients with tumor fragments, we determined normalized expression of MGMT and PTEN genes. In most cases, 53 % of the observations (16 out of 30 patients) showed a low normalized expression of MGMT gene (<40 c. u.) and a low normalized PTEN expression rate of 73 % (22 out of 30 patients) (<40 c. u.). The average expression level of the MGMT gene in the range from 40 to 100 c. u. (6/20 % of patients) was considered prognostic favourable for the response to temozolomide chemotherapy. Conclusions: The study of MGMT gene expression, a chemotherapy marker for temozolomide, indicates a trend toward correlation between expression levels and therapeutic efficacy. The study of the expression of the PTEN gene, the blocker of the PI3K / AKT signal pathway, indicates a different degree of expression of this enzyme in the tumour samples studied. The predictive value of the indicator for target therapy is appropriate in comparison with the EGFR mutation. Further profound analysis of the results is required with increasing number of sampling and observation period.

scholarly journals A PROTOCOL FOR THE DETECTION OF GENETIC MARKERS IN SALIVA BY POLYMERASE CHAIN REACTION WITHOUT A NUCLEIC ACID PURIFICATION STEP: EXAMPLES OF SARS-COV-2 AND GAPDH MARKERS

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Surya Kannan ◽  
◽  
Johan Ericsson ◽  
Nazariy Souchelnytskyi ◽  
Serhiy Souchelnytskyi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acids ◽  
Real Time ◽  
Genetic Markers ◽  
Room Temperature ◽  
Direct Detection ◽  
Clinical Samples ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Introduction. Polymerase chain reaction (PCR)-based diagnostic tests use purifi ed nucleic acids (NAs) from clinical samples. The NAs purifi cation step adds time, cost, and aff ects the quality of testing. The objective of this study was to develop a protocol for direct use of saliva in tests for genetic markers, without purifi cation of nucleic acids. Methods. PCR, real-time RT-PCR and isothermal amplifi cation tests were used for direct detection of genetic markers, without purifi cation of nucleic acids. Results. We report a protocol for the direct detection of genetic markers in saliva. The protocol is based on a collection of saliva in a solution containing a detergent and ethanol and is compatible with isothermal amplifi cation (LAMP), real-time RT-PCR and RT-PCR. SARS-CoV-2 and GAPDH markers were used as reference markers. We observed that mild detergents allow effi cient detection of external reference and intracellular endogenous markers, while strong detergent, e.g. sodium dodecyl sulfate, inhibited the PCR reaction. Under these conditions, saliva samples can be stored for 24 h at +4°C or –18°C with the preservation of markers. Storage at room temperature led to the deterioration of marker detection. Snap heating of saliva samples at the time of collection, followed by storage at room temperature, provided partial protection. Conclusion. The protocol presented in this report describes the collection and storage of saliva for direct detection of genetic markers and is compatible with PCR and LAMP tests.

scholarly journals Identification of 29 Rat Genetic Markers by Arbitrarily Primed Polymerase Chain Reaction

1996 ◽  
Vol 87 (7) ◽  
pp. 669-675 ◽  
Author(s):  
Federico Canzian ◽  
Toshikazu Ushijima ◽  
Minoru Toyota ◽  
Yoko Hosoya ◽  
Takashi Sugimura ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Markers ◽  
Chain Reaction ◽  
Polymerase Chain
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