Differences in efficacy, resistance mechanism and target protein interaction between two PPO inhibitors in Palmer amaranth (Amaranthus palmeri)

Weed Science ◽  
2020 ◽  
Vol 68 (2) ◽  
pp. 105-115
Author(s):  
Chenxi Wu ◽  
Michael-Rock Goldsmith ◽  
John Pawlak ◽  
Paul Feng ◽  
Stacie Smith ◽  
...  
Keyword(s):  
Weed Management ◽  
Management Strategies ◽  
Resistance Mechanism ◽  
Palmer Amaranth ◽  
Target Protein ◽  
Controlled Environment ◽  
Amaranthus Palmeri ◽  
In Planta ◽  
Resistance Development ◽  
Weed Survey

AbstractA weed survey was conducted on 134 Palmer amaranth (Amaranthus palmeri S. Watson) populations from Mississippi and Arkansas in 2017 to investigate the spread of resistance to protoporphyrinogen oxidase (PPO) inhibitors using fomesafen as a proxy. Fomesafen resistance was found in 42% of the A. palmeri populations. To investigate the resistance basis of different PPO inhibitors, we further characterized 10 representative populations by in planta bioassay in a controlled environment and molecular characterizations (DNA sequencing and TaqMan® gene expression assay). A total of 160 plants were sprayed with a labeled field rate (1X) of fomesafen or salfufenacil and screened for the presence of three known resistance-endowing mutations in the mitochondrial PPX2 gene (ΔGly-210, Arg-128-Gly, Gly-399-Ala). To compare the potencies of fomesafen and saflufenacil, dose–response studies were conducted on two highly resistant and one sensitive populations. The interaction of the two herbicides with the target protein harboring known PPX2 mutations was also analyzed. Our results showed that: (1) 90% of the fomesafen- or saflufenacil-resistant plants have at least one of the three known PPX2 mutations, with ΔGly-210 being the most prevalent; (2) saflufenacil is more potent than fomesafen, with five to nine times lower resistance/susceptible (R/S) ratios; (3) fomesafen selects for more diverse mutations, and computational inhibitor/target modeling of fomesafen suggest a weaker binding affinity in addition to a smaller interaction volume and volume overlap with the substrate protoporphyrinogen IX than saflufenacil. As a result, saflufenacil shows reduced sensitivity to PPX2 target-site mutations. Results from current study can help pave the way for designing weed management strategies to delay resistance development and maintain the efficacy of PPO inhibitors.

scholarly journals Effects of Palmer Amaranth (Amaranthus palmeri) Establishment Time and Distance from the Crop Row on Biological and Phenological Characteristics of the Weed: Implications on Soybean Yield

Weed Science ◽  
2019 ◽  
Vol 67 (1) ◽  
pp. 126-135 ◽  
Author(s):  
Nicholas E. Korres ◽  
Jason K. Norsworthy ◽  
Andy Mauromoustakos
Keyword(s):  
Seed Production ◽  
Weed Management ◽  
Cropping Systems ◽  
Management Strategies ◽  
Palmer Amaranth ◽  
Yield Reduction ◽  
Amaranthus Palmeri ◽  
Soybean Yield ◽  
Management Measures ◽  
Weed Population Dynamics

AbstractInformation about weed biology and weed population dynamics is critical for the development of efficient weed management programs. A field experiment was conducted in Fayetteville, AR, during 2014 and 2015 to examine the effects of Palmer amaranth (Amaranthus palmeriS. Watson) establishment time in relation to soybean [Glycine max(L.) Merr.] emergence and the effects ofA. palmeridistance from the soybean row on the weed’s height, biomass, seed production, and flowering time and on soybean yield. The establishment time factor, in weeks after crop emergence (WAE), was composed of six treatment levels (0, 1, 2, 4, 6, and 8 WAE), whereas the distance from the crop consisted of three treatment levels (0, 24, and 48 cm). Differences inA. palmeribiomass and seed production averaged across distance from the crop were found at 0 and 1 WAE in both years. Establishment time had a significant effect onA. palmeriseed production through greater biomass production and height increases at earlier dates.Amaranthus palmerithat was established with the crop (0 WAE) overtopped soybean at about 7 and 10 WAE in 2014 and 2015, respectively. Distance from the crop affectedA. palmeriheight, biomass, and seed production. The greater the distance from the crop, the higherA. palmeriheight, biomass, and seed production at 0 and 1 WAE compared with other dates (i.e., 2, 4, 6, and 8 WAE).Amaranthus palmeriestablishment time had a significant impact on soybean yield, but distance from the crop did not. The earlierA. palmeriinterfered with soybean (0 and 1 WAE), the greater the crop yield reduction; after that period no significant yield reductions were recorded compared with the rest of the weed establishment times. Knowledge ofA. palmeriresponse, especially at early stages of its life cycle, is important for designing efficient weed management strategies and cropping systems that can enhance crop competitiveness. Control ofA. palmeriwithin the first week after crop emergence or reduced distance between crop and weed are important factors for an effective implementation of weed management measures againstA. palmeriand reduced soybean yield losses due to weed interference.

scholarly journals Assessing the Economic Impact of Inversion Tillage, Cover Crops, and Herbicide Regimes in Palmer Amaranth(Amaranthus palmeri)Infested Cotton

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Leah M. Duzy ◽  
Andrew J. Price ◽  
Kipling S. Balkcom ◽  
Jatinder S. Aulakh
Keyword(s):  
Cover Crops ◽  
Weed Management ◽  
Management Strategies ◽  
Palmer Amaranth ◽  
Production Costs ◽  
Integrated Weed Management ◽  
Amaranthus Palmeri ◽  
Cotton Production ◽  
Crimson Clover ◽  
Net Returns

Cotton (Gossypium hirsutumL.) producers in Alabama are faced with a rapidly expanding problem that decreases yields and increases production costs: herbicide-resistant weeds. Producers increasingly rely on integrated weed management strategies that raise production costs. This analysis evaluated how tillage, cover crops, and herbicide regime affected net returns above variable treatment costs (net returns) for cotton production in Alabama from 2009 to 2011 under pressure from Palmer amaranth (Amaranthus palmeriS. Wats.). Annual net returns were compared for two tillage treatments (inversion and noninversion tillage), three cover crops (crimson clover [Trifolium incarnatumL.], cereal rye [Secale cerealL.], and winter fallow), and three herbicide regimes (PRE, POST, and PRE+POST). Results indicate that under heavy Palmer amaranth population densities one year of inversion tillage followed by two years of noninversion tillage, along with a POST or PRE+POST herbicide application had the highest net returns in the first year; however, the economic benefit of inversion tillage, across all herbicide treatments, was nonexistent in 2010 and 2011. Cotton producers with Palmer amaranth infestations would likely benefit from cultural controls, in conjunction with herbicide applications, as part of their weed management system to increase net returns.

Susceptibility of Palmer amaranth (Amaranthus palmeri) to herbicides in accessions collected from the North Carolina Coastal Plain

Weed Science ◽  
2020 ◽  
Vol 68 (6) ◽  
pp. 582-593
Author(s):  
Denis J. Mahoney ◽  
David L. Jordan ◽  
Nilda Roma-Burgos ◽  
Katherine M. Jennings ◽  
Ramon G. Leon ◽  
...  
Keyword(s):  
North Carolina ◽  
Dose Response ◽  
Coastal Plain ◽  
Weed Management ◽  
Acetolactate Synthase ◽  
Fold Increase ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Sites Of Action ◽  
Two Populations

AbstractPalmer amaranth (Amaranthus palmeri S. Watson) populations resistant to acetolactate synthase (ALS)-inhibiting herbicides and glyphosate are fairly common throughout the state of North Carolina (NC). This has led farm managers to rely more heavily on herbicides with other sites of action (SOA) for A. palmeri control, especially protoporphyrinogen oxidase and glutamine synthetase inhibitors. In the fall of 2016, seeds from A. palmeri populations were collected from the NC Coastal Plain, the state’s most prominent agricultural region. In separate experiments, plants with 2 to 4 leaves from the 110 populations were treated with field use rates of glyphosate, glufosinate-ammonium, fomesafen, mesotrione, or thifensulfuron-methyl. Percent visible control and survival were evaluated 3 wk after treatment. Survival frequencies were highest following glyphosate (99%) or thifensulfuron-methyl (96%) treatment. Known mutations conferring resistance to ALS inhibitors were found in populations surviving thifensulfuron-methyl application (Ala-122-Ser, Pro-197-Ser, Trp-574-Leu, and/or Ser-653-Asn), in addition to a new mutation (Ala-282-Asp) that requires further investigation. Forty-two populations had survivors after mesotrione application, with one population having 17% survival. Four populations survived fomesafen treatment, while none survived glufosinate. Dose–response studies showed an increase in fomesafen needed to kill 50% of two populations (LD50); however, these rates were far below the field use rate (less than 5 g ha−1). In two populations following mesotrione dose–response studies, a 2.4- to 3.3-fold increase was noted, with LD90 values approaching the field use rate (72.8 and 89.8 g ha−1). Screening of the progeny of individuals surviving mesotrione confirmed the presence of resistance alleles, as there were a higher number of survivors at the 1X rate compared with the parent population, confirming resistance to mesotrione. These data suggest A. palmeri resistant to chemistries other than glyphosate and thifensulfuron-methyl are present in NC, which highlights the need for weed management approaches to mitigate the evolution and spread of herbicide-resistant populations.

scholarly journals Confirmation of S-metolachlor resistance in Palmer amaranth (Amaranthus palmeri)

2019 ◽  
Vol 33 (5) ◽  
pp. 720-726 ◽  
Author(s):  
Chad Brabham ◽  
Jason K. Norsworthy ◽  
Michael M. Houston ◽  
Vijay K Varanasi ◽  
Tom Barber
Keyword(s):  
Dose Response ◽  
Resistance Mechanism ◽  
Acetolactate Synthase ◽  
Chain Fatty Acid ◽  
Palmer Amaranth ◽  
Cross Resistance ◽  
Amaranthus Palmeri ◽  
Glutathione S Transferase ◽  
Long Chain Fatty Acid ◽  
Long Chain

AbstractS-Metolachlor is commonly used by soybean and cotton growers, especially with POST treatments for overlapping residuals, to obtain season-long control of glyphosate- and acetolactate synthase (ALS)–resistant Palmer amaranth. In Crittenden County, AR, reports of Palmer amaranth escapes following S-metolachlor treatment were first noted at field sites near Crawfordsville and Marion in 2016. Field and greenhouse experiments were conducted to confirm S-metolachlor resistance and to test for cross-resistance to other very-long-chain fatty acid (VLCFA)–inhibiting herbicides in Palmer amaranth accessions from Crawfordsville and Marion. Palmer amaranth control in the field (soil <3% organic matter) 14 d after treatment (DAT) was ≥94% with a 1× rate of acetochlor (1,472 g ai ha–1; emulsifiable concentrate formulation) and dimethenamid-P (631 g ai ha–1). However, S-metolachlor at 1,064 g ai ha–1 provided only 76% control, which was not significantly different from the 1/2× and 1/4× rates of dimethenamid-P and acetochlor (66% to 85%). In the greenhouse, Palmer amaranth accessions from Marion and Crawfordsville were 9.8 and 8.3 times more resistant to S-metolachlor compared with two susceptible accessions based on LD50 values obtained from dose–response experiments. Two-thirds and 1.5 times S-metolachlor at 1,064 g ha–1 were the estimated rates required to obtain 90% mortality of the Crawfordsville and Marion accessions, respectively. Data collected from the field and greenhouse confirm that these accessions have evolved a low level of resistance to S-metolachlor. In an agar-based assay, the level of resistance in the Marion accession was significantly reduced in the presence of a glutathione S-transferase (GST) inhibitor, suggesting that GSTs are the probable resistance mechanism. With respect to other VLCFA-inhibiting herbicides, Marion and Crawfordsville accessions were not cross-resistant to acetochlor, dimethenamid-P, or pyroxasulfone. However, both accessions, based on LD50 values obtained from greenhouse dose–response experiments, exhibited reduced sensitivity (1.5- to 3.6-fold) to the tested VLCFA-inhibiting herbicides.

scholarly journals Evaluation of POST-Harvest Herbicide Applications for Seed Prevention of Glyphosate-Resistant Palmer amaranth (Amaranthus palmeri)

2015 ◽  
Vol 29 (3) ◽  
pp. 405-411 ◽  
Author(s):  
Whitney D. Crow ◽  
Lawrence E. Steckel ◽  
Robert M. Hayes ◽  
Thomas C. Mueller
Keyword(s):  
Weed Management ◽  
Control Strategies ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Wheat Grain ◽  
Herbicide Application ◽  
Post Harvest ◽  
Herbicide Treatments ◽  
Soil Seedbank ◽  
Residual Herbicide

Recent increases in the prevalence of glyphosate-resistant (GR) Palmer amaranth mandate that new control strategies be developed to optimize weed control and crop performance. A field study was conducted in 2012 and 2013 in Jackson, TN, and in 2013 in Knoxville, TN, to evaluate POST weed management programs applied after harvest (POST-harvest) for prevention of seed production from GR Palmer amaranth and to evaluate herbicide carryover to winter wheat. Treatments were applied POST-harvest to corn stubble, with three applications followed by a PRE herbicide applied at wheat planting. Paraquat alone or mixed withS-metolachlor controlled 91% of existing Palmer amaranth 14 d after treatment but did not control regrowth. Paraquat tank-mixed with a residual herbicide of metribuzin, pyroxasulfone, saflufenacil, flumioxazin, pyroxasulfone plus flumioxazin, or pyroxasulfone plus fluthiacet improved control of regrowth or new emergence compared with paraquat alone. All residual herbicide treatments provided similar GR Palmer amaranth control. Through implementation of POST-harvest herbicide applications, the addition of 1,200 seed m−2or approximately 12 million seed ha−1to the soil seedbank was prevented. Overall, the addition of a residual herbicide provided only 4 to 7% more GR Palmer amaranth control than paraquat alone. Wheat injury was evident (< 10%) in 2012 from the PRE applications, but not in 2013. Wheat grain yield was not adversely affected by any herbicide application.

Modeling Glyphosate Resistance Management Strategies for Palmer Amaranth (Amaranthus palmeri) in Cotton

2011 ◽  
Vol 25 (3) ◽  
pp. 335-343 ◽  
Author(s):  
Paul Neve ◽  
Jason K. Norsworthy ◽  
Kenneth L. Smith ◽  
Ian A. Zelaya
Keyword(s):  
Weed Management ◽  
Management Strategies ◽  
Resistance Management ◽  
Glyphosate Resistance ◽  
Palmer Amaranth ◽  
Case Scenario ◽  
Management Options ◽  
Worst Case ◽  
Resistance Risk ◽  
Resistance Evolution

A simulation model is used to explore management options to mitigate risks of glyphosate resistance evolution in Palmer amaranth in glyphosate-resistant cotton in the southern United States. Our first analysis compares risks of glyphosate resistance evolution for seven weed-management strategies in continuous glyphosate-resistant cotton monoculture. In the “worst-case scenario” with five applications of glyphosate each year and no other herbicides applied, evolution of glyphosate resistance was predicted in 74% of simulated populations. In other strategies, glyphosate was applied with various combinations of preplant, PRE, and POST residual herbicides. The most effective strategy included four glyphosate applications with a preplant fomesafen application, and POST tank mixtures of glyphosate plusS-metolachlor followed by glyphosate plus flumioxazin. This strategy reduced the resistance risk to 12% of populations. A second series of simulations compared strategies where glyphosate-resistant cotton was grown in one-to-one rotations with corn or cotton with other herbicide resistance traits. In general, crop rotation reduced risks of resistance by approximately 50% and delayed the evolution of resistance by 2 to 3 yr. These analyses demonstrate that risks of glyphosate resistance evolution in Palmer amaranth can be reduced by reducing glyphosate use within and among years, controlling populations with diverse herbicide modes of action, and ensuring that population size is kept low. However, no strategy completely eliminated the risk of glyphosate resistance.

Evaluation of Herbicide Programs for Use in a 2,4-D–Resistant Soybean Technology for Control of Glyphosate-Resistant Palmer Amaranth (Amaranthus palmeri)

2016 ◽  
Vol 30 (2) ◽  
pp. 366-376 ◽  
Author(s):  
M. Ryan Miller ◽  
Jason K. Norsworthy
Keyword(s):  
Weed Management ◽  
Field Experiments ◽  
Palmer Amaranth ◽  
Integrated Weed Management ◽  
Amaranthus Palmeri ◽  
Early Season ◽  
Fully Integrated ◽  
Soil Seedbank ◽  
High Level ◽  
Herbicide Programs

Two separate field experiments were conducted over a 2-yr period in Fayetteville, AR, during 2012 and 2013 to (1) evaluate POST herbicide programs utilizing a premixture of dimethylamine (DMA) salt of glyphosate + choline salt of 2,4-D in a soybean line resistant to 2,4-D, glyphosate, and glufosinate and (2) determine efficacy of herbicide programs that begin with PRE residual herbicides followed by POST applications of 2,4-D choline + glyphosate DMA on glyphosate-resistant Palmer amaranth. In the first experiment, POST applications alone that incorporated the use of residual herbicides with the glyphosate + 2,4-D premixture provided 93 to 99% control of Palmer amaranth at the end of the season. In the second experiment, the use of flumioxazin, flumioxazin + chlorimuron methyl, S-metolachlor + fomesafen, or sulfentrazone + chloransulam applied PRE provided 94 to 98% early-season Palmer amaranth control. Early-season control helped maintain a high level of Palmer amaranth control throughout the growing season, in turn resulting in fewer reproductive Palmer amaranth plants present at soybean harvest compared to most other treatments. Although no differences in soybean yield were observed among treated plots, it was evident that herbicide programs should begin with PRE residual herbicides followed by POST applications of glyphosate + 2,4-D mixed with residual herbicides to minimize late-season escapes and reduce the likelihood of contributions to the soil seedbank. Dependent upon management decisions, the best stewardship of this technology will likely rely on the use multiple effective mechanisms of action incorporated into a fully integrated weed management system.

Biotypes of Palmer Amaranth (Amaranthus palmeri) and Common Waterhemp (Amaranthus rudis) are Resistant to Imazethapyr and Thifensulfuron

1995 ◽  
Vol 9 (1) ◽  
pp. 192-195 ◽  
Author(s):  
Michael J. Horak ◽  
Dallas E. Peterson
Keyword(s):  
Herbicide Resistance ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Typical Pattern ◽  
Resistance Development ◽  
Common Waterhemp ◽  
Herbicide Resistant ◽  
Amaranthus Rudis ◽  
History Of ◽  
History Of Use

Seeds of suspected herbicide-resistant Palmer amaranth and common waterhemp were collected in Clay County and Douglas County, KS, respectively. An experiment was established in a greenhouse to determine if these species had developed resistance to imazethapyr and thifensulfuron. Imazethapyr was applied pre- (PRE) and postemergence (POST) at 1×, 2×, 4×, and 8× the suggested use rate (70 g/ha), and thifensulfuron was applied POST at 1×, 2×, 4×, and 8× the suggested use rate (4.5 g/ha). Both species had developed resistance to all rates of these herbicides. The occurrence of resistance at the Clay County site (Palmer amaranth) fit the typical pattern for the development of herbicide resistance, i.e., multiple applications of the same class of herbicide for several years. However, the Douglas County (common waterhemp) site had a limited history of use of ALS-inhibiting herbicides and did not follow typical models of resistance development.

Response of a Multiple-Resistant Palmer Amaranth (Amaranthus palmeri) Population to Four HPPD-Inhibiting Herbicides Applied Alone and with Atrazine

Weed Science ◽  
2017 ◽  
Vol 65 (4) ◽  
pp. 534-545 ◽  
Author(s):  
Jonathon R. Kohrt ◽  
Christy L. Sprague
Keyword(s):  
Weed Management ◽  
Palmer Amaranth ◽  
Amaranthus Palmeri ◽  
Joint Activity ◽  
Application Timing ◽  
Greenhouse Experiments ◽  
Synergistic Response

Control of multiple-resistant Palmer amaranth populations in corn will rely heavily on the use of POST 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides. Therefore, field and greenhouse experiments were conducted to: (1) evaluate Palmer amaranth control with four HPPD inhibitors alone and in combination with atrazine at two application timings and (2) investigate the joint activity of HPPD-inhibiting herbicides and atrazine in atrazine-resistant (AR) and atrazine-susceptible (AS) Palmer amaranth populations. Control of the AR Palmer amaranth population varied among the HPPD-inhibiting herbicides with tolpyralate>tembotrione=topramezone>mesotrione based on GR50values in the greenhouse. In the field, Palmer amaranth control was lower when the HPPD-inhibiting herbicides, with the exception of tolpyralate, were applied to 15- vs. 8-cm-tall Palmer amaranth. Tolpyralate controlled Palmer amaranth ≥95% at both application timings. The addition of atrazine (560 g ai ha−1) improved Palmer amaranth control with mesotrione and topramezone at the 8-cm application timing and with mesotrione and tembotrione at the 15-cm application timing. In the greenhouse, joint activity of mesotrione and atrazine and tembotrione and atrazine was synergistic with both the AR and AS Palmer amaranth populations. In the AR population, an additional 980 g ai ha−1of atrazine (8X) was needed to cause a synergistic response compared with the AS population. Synergistic responses with mesotrione were detected with all atrazine rates for the AS population and for atrazine rates ranging from 280 to 2,240 g ai ha−1for the AR population. Only additive responses were observed when atrazine was applied with tolpyralate and topramezone, indicating that joint activity in the form of synergism occurs more readily with the triketones compared with the benzopyrazoles. When faced with an AR Palmer amaranth population, the addition of atrazine to HPPD inhibitors may increase the overall success of weed management due to joint activity.

Glyphosate-Resistant Palmer Amaranth (Amaranthus palmeri) Increases Herbicide Use, Tillage, and Hand-Weeding in Georgia Cotton

Weed Science ◽  
2014 ◽  
Vol 62 (2) ◽  
pp. 393-402 ◽  
Author(s):  
Lynn M. Sosnoskie ◽  
A. Stanley Culpepper
Keyword(s):  
Weed Management ◽  
Glyphosate Resistance ◽  
Palmer Amaranth ◽  
Extension Agents ◽  
Amaranthus Palmeri ◽  
Field Site ◽  
Timely Manner ◽  
Biological Attributes ◽  
Hand Weeding ◽  
Herbicide Use

In 2005, the existence of glyphosate-resistance in Palmer amaranth was confirmed at a single 250 ha field site in Macon County, Georgia. Currently, all cotton producing counties in Georgia are infested, to some degree, with glyphosate-resistant Palmer amaranth. In 2010 and 2011, surveys were administered to Georgia growers and extension agents to determine how the development of glyphosate-resistance has affected weed management in cotton. According to respondents, the numbers of cotton acres that were treated with paraquat, glufosinate and residual herbicides effective against Palmer amaranth more than doubled between 2000 to 2005 and 2006 to 2010. Glyphosate use declined between 2000 to 2005 and 2006 to 2010 although, on average, the active ingredient was still applied to a majority of cotton acres. Although grower herbicide input costs have more than doubled following the evolution and spread of glyphosate resistance, chemically-based control of Palmer amaranth is still not adequate. As a consequence, Georgia cotton growers hand weeded 52% of the crop at an average cost of $57 per hand-weeded ha; this represents a cost increase of at least 475% as compared to the years prior to resistance. In addition to increased herbicide use and hand weeding, growers in Georgia are also using mechanical, in-crop cultivation (44% of acres), tillage for the incorporation of preplant herbicides (20% of the acres), and post-harvest deep-turning (19% of the acres every three years) for weed control. Current weed management systems are more diverse, complex and expensive than those employed only a decade ago, but are effective at controlling glyphosate-resistant Palmer amaranth in glyphosate-resistant cotton. The success of these programs may be related to producers improved knowledge about herbicide resistance, and the biological attributes that make Palmer amaranth so challenging, as well as their ability to implement their management programs in a timely manner.

Sign in / Sign up

Export Citation Format

Share Document