Physiological assessment of non–target site restistance in multiple-resistant junglerice (Echinochloa colona)

Weed Science ◽  
2019 ◽  
Vol 67 (6) ◽  
pp. 622-632 ◽  
Author(s):  
Christopher E. Rouse ◽  
Nilda Roma-Burgos ◽  
Bianca Assis Barbosa Martins
Keyword(s):  
Management Practices ◽  
Enzyme Inhibitors ◽  
Treatment Resistance ◽  
Target Site ◽  
Metabolic Enzyme ◽  
Herbicide Resistant ◽  
Whole Plant ◽  
Detoxification Mechanism ◽  
Target Site Resistance ◽  
Resistance To Herbicides

AbstractHerbicide-resistant Echinochloa species are among the most problematic weeds in agricultural crops globally. Recurring herbicide selection pressure in the absence of diverse management practices has resulted in greater than 20% of sampled Echinochloa populations from rice (Oryza sativa L.) fields demonstrating multiple resistance to herbicides in Arkansas, USA. We assessed the resistance profile and potential mechanisms of resistance in a multiple herbicide–resistant junglerice [Echinochloa colona (L.) Link] (ECO-R) population. Whole-plant and laboratory bioassays were conducted to identify the potential mechanisms of non–target site resistance in this population. ECO-R was highly resistant to propanil (>37,800 g ha−1) and quinclorac (>17,920 g ha−1) and had elevated tolerance to cyhalofop (R/S = 1.9) and glufosinate (R/S = 1.2) compared to the susceptible standard. The addition of glufosinate (590 g ha−1) to cyhalofop (314 g ha−1), propanil (4,500 g ha−1), or quinclorac (560 g ha−1) controlled ECO-R 100%. However, cyhalofop applied with propanil (48% control) or quinclorac (15% control) was antagonistic. The application of the known metabolic enzyme inhibitors malathion, carbaryl, and piperonyl butoxide increased control of ECO-R with propanil (>75%) but not with other herbicides. Neither absorption nor translocation of [14C]cyhalofop or propanil was different between ECO-R and ECO-S. [14C]Quinclorac absorption was also similar between ECO-R and ECO-S; however, translocation of quinclorac into tissues above the treated leaf of ECO-R was >20% higher than that in ECO-S. The abundance of metabolites was higher (∼10%) in the treated leaves of ECO-R than in ECO-S beginning 48 h after treatment. The activity of β-cyanoalanine synthase, which detoxifies hydrogen cyanide, was not different between ECO-R and ECO-S following quinclorac treatment. Resistance to propanil was due to herbicide detoxification by metabolic enzymes. Resistance to quinclorac was due to a detoxification mechanism yet to be understood. The reduction in sensitivity to cyhalofop and glufosinate might be a secondary effect of the mechanisms conferring high resistance to propanil and quinclorac.

Controlling Herbicide-Resistant Annual Bluegrass (Poa annua) Phenotypes with Methiozolin

2017 ◽  
Vol 31 (3) ◽  
pp. 470-476 ◽  
Author(s):  
James T. Brosnan ◽  
Jose J. Vargas ◽  
Gregory K. Breeden ◽  
Sarah L. Boggess ◽  
Margaret A. Staton ◽  
...  
Keyword(s):  
Acetolactate Synthase ◽  
Target Site ◽  
Multiple Resistance ◽  
Herbicide Resistant ◽  
Annual Bluegrass ◽  
Microtubule Inhibitors ◽  
Effective Option ◽  
Target Site Resistance ◽  
Susceptible Control ◽  
Als Inhibitors

Methiozolin is an isoxazoline herbicide being investigated for selective POST annual bluegrass control in managed turfgrass. Research was conducted to evaluate methiozolin efficacy for controlling two annual bluegrass phenotypes with target-site resistance to photosystem II (PSII) or enolpyruvylshikimate-3-phosphate synthase (EPSPS)-inhibiting herbicides (i.e., glyphosate), as well as phenotypes with multiple resistance to microtubule and EPSPS or PSII and acetolactate synthase (ALS)-inhibiting herbicides. All resistant phenotypes were established in glasshouse culture along with a known herbicide-susceptible control and treated with methiozolin at 0, 125, 250, 500, 1000, 2000, 4000, or 8000 g ai ha−1. Methiozolin effectively controlled annual bluegrass with target-site resistance to inhibitors of EPSPS, PSII, as well as multiple resistance to EPSPS and microtubule inhibitors. Methiozolin rates required to reduce aboveground biomass of these resistant phenotypes 50% (GR50 values) were not significantly different from the susceptible control, ranging from 159 to 421 g ha−1. A phenotype with target-site resistance to PSII and ALS inhibitors was less sensitive to methiozolin (GR50=862 g ha−1) than a susceptible phenotype (GR50=423 g ha−1). Our findings indicate that methiozolin is an effective option for controlling select annual bluegrass phenotypes with target-site resistance to several herbicides.

scholarly journals Molecular Mechanisms of Herbicide Resistance

Weed Science ◽  
2015 ◽  
Vol 63 (SP1) ◽  
pp. 91-115 ◽  
Author(s):  
Christophe Délye ◽  
Arnaud Duhoux ◽  
Fanny Pernin ◽  
Chance W. Riggins ◽  
Patrick J. Tranel
Keyword(s):  
Herbicide Resistance ◽  
Molecular Mechanisms ◽  
Target Site ◽  
Target Protein ◽  
Intrinsic Activity ◽  
Evolutionary Adaptation ◽  
Herbicide Binding ◽  
Target Site Resistance ◽  
Resistance To Herbicides ◽  
Increased Activity

Resistance to herbicides occurs in weeds as the result of evolutionary adaptation (Jasieniuk et al. 1996). Basically, two types of mechanisms are involved in resistance (Beckie and Tardif 2012; Délye 2013). Target-site resistance (TSR) is caused by changes in the tridimensional structure of the herbicide target protein that decrease herbicide binding, or by increased activity (e.g., due to increased expression or increased intrinsic activity) of the target protein. Nontarget-site resistance (NTSR) is endowed by any mechanism not belonging to TSR, e.g., reduction in herbicide uptake or translocation in the plant, or enhanced herbicide detoxification (reviewed in Délye 2013; Yuan et al. 2007).

Segregation of non-target-site resistance to herbicides in multiple-resistantAlopecurus myosuroidesplants

Weed Research ◽  
2015 ◽  
Vol 55 (3) ◽  
pp. 298-308 ◽  
Author(s):  
M Rosenhauer ◽  
F G Felsenstein ◽  
H-P Piepho ◽  
M Höfer ◽  
J Petersen
Keyword(s):  
Target Site ◽  
Target Site Resistance ◽  
Resistance To Herbicides

scholarly journals Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation

2012 ◽  
Vol 30 (3) ◽  
pp. 675-681 ◽  
Author(s):  
M.D. Osuna ◽  
I.C.G.R. Goulart ◽  
R.A. Vidal ◽  
A. Kalsing ◽  
J.P. Ruiz Santaella ◽  
...  
Keyword(s):  
Amino Acid Position ◽  
Target Site ◽  
In Vitro Assays ◽  
Site Mutation ◽  
Eleusine Indica ◽  
Herbicide Resistant ◽  
Frequent Use ◽  
Target Site Resistance ◽  
Grass Weed

Eleusine indica (goosegrass) is a diploid grass weed which has developed resistance to ACCase inhibitors during the last ten years due to the intensive and frequent use of sethoxydim to control grass weeds in soybean crops in Brazil. Plant dose-response assays confirmed the resistant behaviour of one biotype obtaining high resistance factor values: 143 (fenoxaprop), 126 (haloxyfop), 84 (sethoxydim) to 58 (fluazifop). ACCase in vitro assays indicated a target site resistance as the main cause of reduced susceptibility to ACCase inhibitors. PCR-generated fragments of the ACCase CT domain of the resistant and sensitive reference biotype were sequenced and compared. A point mutation was detected within the triplet of aspartate at the amino acid position 2078 (referred to EMBL accession no. AJ310767) and resulted in the triplet of glycine. These results constitute the first report on a target site mutation for a Brazilian herbicide resistant grass weed.

scholarly journals Herbicide Resistance and Management Options of Papaver rhoeas L. and Centaurea cyanus L. in Europe: A Review

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 874
Author(s):  
Marta Stankiewicz-Kosyl ◽  
Agnieszka Synowiec ◽  
Małgorzata Haliniarz ◽  
Anna Wenda-Piesik ◽  
Krzysztof Domaradzki ◽  
...  
Keyword(s):  
Herbicide Resistance ◽  
Acetolactate Synthase ◽  
Agricultural Landscapes ◽  
Target Site ◽  
Management Options ◽  
Papaver Rhoeas ◽  
Herbicide Resistant ◽  
Mediterranean Countries ◽  
Centaurea Cyanus ◽  
Target Site Resistance

Corn poppy (Papaver rhoeas L.) and cornflower (Centaurea cyanus L.) are two overwintering weed species found in crop fields in Europe. They are characterised by a similar life cycle, similar competitive efforts, and a spectrum of herbicides recommended for their control. This review summarises the biology and herbicide resistance phenomena of corn poppy and cornflower in Europe. Corn poppy is one of the most dangerous dicotyledonous weeds, having developed herbicide resistance to acetolactate synthase inhibitors and growth regulators, especially in Mediterranean countries and Great Britain. Target site resistance to acetolactate synthase inhibitors dominates among herbicide-resistant poppy biotypes. The importance of non-target site resistance to acetolactate synthase inhibitors in this species may be underestimated because non-target site resistance is very often associated with target site resistance. Cornflower, meanwhile, is increasingly rare in European agricultural landscapes, with acetolactate synthase inhibitors-resistant biotypes only listed in Poland. However, the mechanisms of cornflower herbicide resistance are not well recognised. Currently, herbicides mainly from acetolactate synthase and photosystem II inhibitors as well as from synthetic auxins groups are recommended for the control of both weeds. Integrated methods of management of both weeds, especially herbicide-resistant biotypes, continue to be underrepresented.

scholarly journals Origin, mechanism and molecular basis of weed resistance to herbicides

2010 ◽  
Vol 40 (No. 4) ◽  
pp. 151-168 ◽  
Author(s):  
D. Chodová ◽  
J. Mikulka ◽  
M. Kočová ◽  
J. Salava
Keyword(s):  
D1 Protein ◽  
Acetolactate Synthase ◽  
Target Site ◽  
Gene Encoding ◽  
Genetic Modifications ◽  
The World ◽  
Target Sites ◽  
Target Site Resistance ◽  
Resistance To Herbicides ◽  
Weed Resistance

This review summarises information from the literature and experimental experience of the authors in research on weed resistance to herbicides. Factors conditioning the origin of resistance are described. The origin of resistant weeds to nine active ingredients with a different mode of action is presented chronologically, and the distribution of resistant weeds around the world outlined. The fundamental modes of action: reduction of the target site sensitivity, so-called "target site resistance", and the mode by which a herbicide is metabolised into inactive products, are listed. Function and genetic modifications of target sites of selected herbicides are described. Czech biotypes of resistant weeds with a mutation at codon 264 of the psbA gene encoding the D1 protein and at codon 574 of the acetolactate synthase gene are presented.

scholarly journals Non-Target-Site Resistance to Herbicides: Recent Developments

Plants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 417 ◽  
Author(s):  
Jugulam ◽  
Shyam
Keyword(s):  
Weed Management ◽  
Management Strategies ◽  
Target Site ◽  
Cross Resistance ◽  
Weed Species ◽  
Physiological Processes ◽  
Recent Developments ◽  
Target Site Resistance ◽  
Resistance To Herbicides ◽  
Selection Of

Non-target-site resistance (NTSR) to herbicides in weeds can be conferred as a result of the alteration of one or more physiological processes, including herbicide absorption, translocation, sequestration, and metabolism. The mechanisms of NTSR are generally more complex to decipher than target-site resistance (TSR) and can impart cross-resistance to herbicides with different modes of action. Metabolism-based NTSR has been reported in many agriculturally important weeds, although reduced translocation and sequestration of herbicides has also been found in some weeds. This review focuses on summarizing the recent advances in our understanding of the physiological, biochemical, and molecular basis of NTSR mechanisms found in weed species. Further, the importance of examining the co-existence of TSR and NTSR for the same herbicide in the same weed species and influence of environmental conditions in the altering and selection of NTSR is also discussed. Knowledge of the prevalence of NTSR mechanisms and co-existing TSR and NTSR in weeds is crucial for designing sustainable weed management strategies to discourage the further evolution and selection of herbicide resistance in weeds.

Target-Site Resistance to Nicosulfuron in Johnsongrass (Sorghum halepense) from Chilean Corn Fields

Weed Science ◽  
2015 ◽  
Vol 63 (3) ◽  
pp. 631-640 ◽  
Author(s):  
María J. Hernández ◽  
Rocío León ◽  
Albert J. Fischer ◽  
Marlene Gebauer ◽  
Rafael Galdames ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Consensus Sequence ◽  
Target Site ◽  
Sorghum Halepense ◽  
Acetohydroxyacid Synthase ◽  
Sequencing Analysis ◽  
Whole Plant ◽  
Target Site Resistance ◽  
Selection For

Johnsongrass is a common weed of corn in Chile, which is most often controlled by nicosulfuron, an acetohydroxyacid synthase (AHAS)-inhibiting herbicide. Recurrent nicosulfuron use has resulted in selection for resistant johnsongrass biotypes. We conducted studies to determine nicosulfuron resistance levels in two johnsongrass biotypes from Chile and to investigate if this resistance was target-site mediated. Whole-plant resistance to nicosulfuron was 33 and 46 times higher in resistant (R) than in susceptible (S) plants grown from seed and rhizomes, respectively. The nicosulfuron concentrations for 50% inhibition of AHAS enzyme activity in vitro were more than 11 times higher in R than in S plants. Sequencing analysis of theAHAScoding sequence revealed a Trp-574-Leu substitution in both R biotypes. This study shows that resistance to nicosulfuron in the two R biotypes is conferred by an altered target site. We also report the first consensus sequence of the johnsongrassAHASgene corresponding to the known mutation sites conferring resistance to AHAS-inhibiting herbicides.

scholarly journals Weed resistance to herbicides

2020 ◽  
Vol 29 (2) ◽  
pp. 79-96
Author(s):  
Sava Vrbničanin
Keyword(s):  
Herbicide Resistance ◽  
Amaranthus Retroflexus ◽  
Ambrosia Artemisiifolia ◽  
Target Site ◽  
Weed Species ◽  
Ps Ii ◽  
Target Site Resistance ◽  
Resistance To Herbicides ◽  
Als Inhibitors ◽  
Weed Resistance

Weed resistance to herbicides represents the acquired resistance of individuals to complete the life cycle and leave offspring in the conditions of extended exposure to the same herbicide, i.e. herbicides of the same mechanism of action to which they were sensitive at the beginning of the application. Based on the herbicide resistance mechanisms, all processes can be grouped as follows: target-site resistance, non-target-site resistance, cross-resistance and multiple-resistance. Currently, herbicide resistance has been reported in 514 cases (species x site of action) worldwide, in 262 weed species (152 dicotyledons, 110 monocotyledons). Many of those biotypes are resistant to als inhibitors, PS II inhibitors, EPSPS inhibitors and ACC-ase inhibitors. The higher degree of resistance to als inhibitors has been confirmed in the following weed species: Amaranthus retroflexus, Sorghum halepense, Ambrosia artemisiifolia and Helianthus annuus.

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