Development of High Levels of Metribuzin Tolerance in Lentil

Weed Science ◽  
2018 ◽  
Vol 67 (1) ◽  
pp. 83-90 ◽  
Author(s):  
Larn S. McMurray ◽  
Christopher Preston ◽  
Albert Vandenberg ◽  
Dili Mao ◽  
Klaus H. Oldach ◽  
...  
Keyword(s):  
Lens Culinaris ◽  
Dry Weight ◽  
Relative Increase ◽  
Crop Species ◽  
Breeding Programs ◽  
Germplasm Screening ◽  
Germplasm Line ◽  
Alternative Weed Control ◽  
High Level ◽  
Mutant Lines

AbstractLentil (Lens culinarisMedik.) is an important and expanding crop in southern Australia and a significant crop in western Canada. Currently, production in both countries is limited by an inability to effectively control weeds, due in part to a lack of registered safe and effective herbicides. Metribuzin is a broad-spectrum herbicide providing an alternative weed control option to the imidazolinones, but it has low crop safety in lentil. Two methods, germplasm screening using a hydroponic sand assay and field screening of a large mutated population of the Australian cultivar ‘PBA Flash’ were initially used to identify lines with putative metribuzin tolerance over current cultivars. Dose–response experiments showed the germplasm line SP1333 had GR50(the rate required to reduce dry weight 50%) values up to four times higher than PBA Flash. However, the mutation selections M043 and M009 had GR50values more than 25 times higher than PBA Flash. A field study in Canada, under conditions of induced shade and no shade 72 h before POST application of metribuzin, confirmed the intermediate level of tolerance in SP1333 and the high level in the two mutant lines compared with 20 Canadian and Australian genotypes. This relative increase in metribuzin tolerance of the two mutant lines over the parent cultivar is higher than all previous reports in a range of crop species. The development of large mutant populations combined with large M2field screens was a successful method for developing high levels of metribuzin tolerance in lentil. The estimated mutation rate of the mutant lines was 9.4×10−8. All three lines are currently being used as parents in lentil breeding programs.

scholarly journals Combining metabolomic and transcriptomic approaches to assess and improve crop quality traits

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Delphine M. Pott ◽  
Sara Durán-Soria ◽  
Sonia Osorio ◽  
José G. Vallarino
Keyword(s):  
Quality Trait ◽  
Quality Traits ◽  
Crop Species ◽  
Breeding Programs ◽  
Crop Quality ◽  
Crop Varieties ◽  
New Genes ◽  
Technical Advances ◽  
Health Promoting ◽  
Selection Of

AbstractPlant quality trait improvement has become a global necessity due to the world overpopulation. In particular, producing crop species with enhanced nutrients and health-promoting compounds is one of the main aims of current breeding programs. However, breeders traditionally focused on characteristics such as yield or pest resistance, while breeding for crop quality, which largely depends on the presence and accumulation of highly valuable metabolites in the plant edible parts, was left out due to the complexity of plant metabolome and the impossibility to properly phenotype it. Recent technical advances in high throughput metabolomic, transcriptomic and genomic platforms have provided efficient approaches to identify new genes and pathways responsible for the extremely diverse plant metabolome. In addition, they allow to establish correlation between genotype and metabolite composition, and to clarify the genetic architecture of complex biochemical pathways, such as the accumulation of secondary metabolites in plants, many of them being highly valuable for the human diet. In this review, we focus on how the combination of metabolomic, transcriptomic and genomic approaches is a useful tool for the selection of crop varieties with improved nutritional value and quality traits.

scholarly journals Arabidopsis Genes Essential for Seedling Viability: Isolation of Insertional Mutants and Molecular Cloning

Genetics ◽  
2001 ◽  
Vol 159 (4) ◽  
pp. 1765-1778
Author(s):  
Gregory J Budziszewski ◽  
Sharon Potter Lewis ◽  
Lyn Wegrich Glover ◽  
Jennifer Reineke ◽  
Gary Jones ◽  
...  
Keyword(s):  
Large Scale ◽  
Protein Translocation ◽  
Gene Families ◽  
Mutant Phenotype ◽  
Lethal Mutant ◽  
A Genome ◽  
Genes Encoding ◽  
High Level ◽  
Mutant Lines ◽  
Genome Scale

Abstract We have undertaken a large-scale genetic screen to identify genes with a seedling-lethal mutant phenotype. From screening ~38,000 insertional mutant lines, we identified >500 seedling-lethal mutants, completed cosegregation analysis of the insertion and the lethal phenotype for >200 mutants, molecularly characterized 54 mutants, and provided a detailed description for 22 of them. Most of the seedling-lethal mutants seem to affect chloroplast function because they display altered pigmentation and affect genes encoding proteins predicted to have chloroplast localization. Although a high level of functional redundancy in Arabidopsis might be expected because 65% of genes are members of gene families, we found that 41% of the essential genes found in this study are members of Arabidopsis gene families. In addition, we isolated several interesting classes of mutants and genes. We found three mutants in the recently discovered nonmevalonate isoprenoid biosynthetic pathway and mutants disrupting genes similar to Tic40 and tatC, which are likely to be involved in chloroplast protein translocation. Finally, we directly compared T-DNA and Ac/Ds transposon mutagenesis methods in Arabidopsis on a genome scale. In each population, we found only about one-third of the insertion mutations cosegregated with a mutant phenotype.

scholarly journals Wheat, Barley, and Oat Breeding for Health Benefit Components in Grain

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 86
Author(s):  
Igor G. Loskutov ◽  
Elena K. Khlestkina
Keyword(s):  
Bioactive Compounds ◽  
Plant Genetic Resources ◽  
Health Benefit ◽  
Cultivated Plants ◽  
Crop Species ◽  
Breeding Programs ◽  
Cereal Grain ◽  
Staple Foods ◽  
Functional Components ◽  
Oat Breeding

Cereal grains provide half of the calories consumed by humans. In addition, they contain important compounds beneficial for health. During the last years, a broad spectrum of new cereal grain-derived products for dietary purposes emerged on the global food market. Special breeding programs aimed at cultivars utilizable for these new products have been launched for both the main sources of staple foods (such as rice, wheat, and maize) and other cereal crops (oat, barley, sorghum, millet, etc.). The breeding paradigm has been switched from traditional grain quality indicators (for example, high breadmaking quality and protein content for common wheat or content of protein, lysine, and starch for barley and oat) to more specialized ones (high content of bioactive compounds, vitamins, dietary fibers, and oils, etc.). To enrich cereal grain with functional components while growing plants in contrast to the post-harvesting improvement of staple foods with natural and synthetic additives, the new breeding programs need a source of genes for the improvement of the content of health benefit components in grain. The current review aims to consider current trends and achievements in wheat, barley, and oat breeding for health-benefiting components. The sources of these valuable genes are plant genetic resources deposited in genebanks: landraces, rare crop species, or even wild relatives of cultivated plants. Traditional plant breeding approaches supplemented with marker-assisted selection and genetic editing, as well as high-throughput chemotyping techniques, are exploited to speed up the breeding for the desired genotуpes. Biochemical and genetic bases for the enrichment of the grain of modern cereal crop cultivars with micronutrients, oils, phenolics, and other compounds are discussed, and certain cases of contributions to special health-improving diets are summarized. Correlations between the content of certain bioactive compounds and the resistance to diseases or tolerance to certain abiotic stressors suggest that breeding programs aimed at raising the levels of health-benefiting components in cereal grain might at the same time match the task of developing cultivars adapted to unfavorable environmental conditions.

scholarly journals Simple Sequence Repeat and S-Locus Genotyping to Assist the Genetic Characterization and Breeding of Polyploid Prunus Species, P. spinosa and P. domestica subsp. insititia

2021 ◽  
Author(s):  
Júlia Halász ◽  
Noémi Makovics-Zsohár ◽  
Ferenc Szőke ◽  
Sezai Ercisli ◽  
Attila Hegedűs
Keyword(s):  
Simple Sequence Repeat ◽  
Principal Component ◽  
Sequence Repeat ◽  
Breeding Programs ◽  
Allele Number ◽  
Genetic Potential ◽  
Ssr Loci ◽  
High Level ◽  
Diversity Studies ◽  
Simple Sequence

AbstractPolyploid Prunus spinosa (2n = 4 ×) and P. domestica subsp. insititia (2n = 6 ×) represent enormous genetic potential in Central Europe, which can be exploited in breeding programs. In Hungary, 16 cultivar candidates and a recognized cultivar ‘Zempléni’ were selected from wild-growing populations including ten P. spinosa, four P. domestica subsp. insititia and three P. spinosa × P. domestica hybrids (2n = 5 ×) were also created. Genotyping in eleven simple sequence repeat (SSR) loci and the multiallelic S-locus was used to characterize genetic variability and achieve a reliable identification of tested accessions. Nine SSR loci proved to be polymorphic and eight of those were highly informative (PIC values ˃ 0.7). A total of 129 SSR alleles were identified, which means 14.3 average allele number per locus and all accessions but two clones could be discriminated based on unique SSR fingerprints. A total of 23 S-RNase alleles were identified and the complete and partial S-genotype was determined for 10 and 7 accessions, respectively. The DNA sequence was determined for a total of 17 fragments representing 11 S-RNase alleles. ‘Zempléni’ was confirmed to be self-compatible carrying at least one non-functional S-RNase allele (SJ). Our results indicate that the S-allele pools of wild-growing P. spinosa and P. domestica subsp. insititia are overlapping in Hungary. Phylogenetic and principal component analyses confirmed the high level of diversity and genetic differentiation present within the analysed accessions and indicated putative ancestor–descendant relationships. Our data confirm that S-locus genotyping is suitable for diversity studies in polyploid Prunus species but non-related accessions sharing common S-alleles may distort phylogenetic inferences.

INHERITANCE OF RESISTANCE TO NET BLOTCH IN BARLEY. I. FACTORS AFFECTING THE PENETRANCE AND EXPRESSIVITY OF GENE(S) CONDITIONING HOST RESISTANCE

1969 ◽  
Vol 11 (3) ◽  
pp. 587-591 ◽  
Author(s):  
T. N. Khan
Keyword(s):  
Environmental Conditions ◽  
Net Blotch ◽  
Host Reaction ◽  
Susceptible Parent ◽  
Breeding Programs ◽  
Genetic Studies ◽  
Factors Affecting ◽  
Drechslera Teres ◽  
Resistant Varieties ◽  
High Level

Variability in the host-reaction of barley to infection by Drechslera teres was examined in the parents and progeny of selected crosses under different environmental conditions of testing.The Ethiopian variety C.I. 5791 exhibits a consistently high level of resistance under a range of environmental conditions, which is in contrast to the Manchurian variety C.I. 2330. The sensitivity of the genes for resistance possessed by these varieties to environmental modifications is considered to depend upon their respective genetic backgrounds. Furthermore, variability of host reaction in the progeny of these resistant varieties was shown to be influenced by the genetic background of the susceptible parent used.The implications of these findings in the conduct and interpretation of genetic studies and in backcross breeding programs is discussed.

scholarly journals Sulfur dioxide-enhanced Phytotoxicity of Ozone to Watermelon

1996 ◽  
Vol 121 (4) ◽  
pp. 716-721 ◽  
Author(s):  
Gwendolyn Eason ◽  
Richard A. Reinert ◽  
James E. Simon
Keyword(s):  
Sulfur Dioxide ◽  
Citrullus Lanatus ◽  
Dry Weight ◽  
Point Sources ◽  
Continuous Stirred Tank Reactor ◽  
Foliar Injury ◽  
Crop Species ◽  
Total Plant ◽  
Continuous Stirred Tank ◽  
The Impact

Three watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai] cultivars with different ozone (O3) sensitivities were grown in a charcoal-filtered greenhouse and exposed in continuous-stirred tank reactor chambers to five levels (0, 100, 200, 300, or 400 nL·L-1) of sulfur dioxide (SO2) in the presence (80 nL·L-1) or absence (0 nL·L-1) of ozone (O3) for 4 hours/day, 5 days/week for 22 days. In the presence of O3, SO2 increased foliar injury in all three cultivars, but the impact was greatest for the most O3-sensitive cultivar, `Sugar Baby,' moderate for `Crimson Sweet,' and least for the least O3-sensitive cultivar, `Charleston Gray.' For all cultivars, SO2 intensified O3 suppression of leaf area for the first seven mainstem leaves and of dry weights for aboveground and total plant tissues. Root dry weight was independently suppressed by both pollutants, and the root: top ratio was linearly suppressed by SO2 alone. Sulfur dioxide combined with O3 can be detrimental to crop species such as watermelon. Thus, the potential for SO2 phytotoxicity should not be summarily dismissed, especially in the vicinity of SO2 point sources where O3 co-occurs.

scholarly journals Rust and leaf spot diseases on American elderberry plants

2017 ◽  
Author(s):  
◽  
Kaley Hensel
Keyword(s):  
Leaf Spot ◽  
Dry Weight ◽  
Growing Season ◽  
Significant Loss ◽  
Leaf Wetness ◽  
Conservative Estimate ◽  
Sambucus Nigra ◽  
Shoot Dry Weight ◽  
Harvest Dates ◽  
High Level

Elderberry rust (Puccinia sambuci Schewin.) Arthur (=P. bolleyana) and leaf spot diseases are frequently found in commercial American elderberry (Sambucus nigra L. subsp. canadensis L.) plantings throughout the growing season in Missouri. Thus, studies were conducted to ascertain if rust infections affect plant growth, fruiting, or berry puree quality. Rust symptoms were observed in early April at 9 to 18[degrees] C, [greater than or equal to] 3 h leaf wetness, and [greater than or equal to] 85% relative humidity. When young, potted elderberry plants averaged 3 to 6 rust pustules/plant, vegetative growth was not adversely affected. However, field-grown elderberry plants heavily infected with rust (137 pustules/cane) lost nearly twice as many leaves as controls during the growing season, indicating rust-induced defoliation. Shoot dry weight of these heavily infected canes was also 32% less than that of controls. First and last harvest dates were advanced by the high level of rust infection on 'Wyldewood' elderberry canes, but not by low pustules numbers ([less than] 6 pustules/plant) on 'Bob Gordon' or 'Ozark' plants. Similarly, berry yields were not significantly different at low infection levels, even though rust-infected 'Bob Gordon' plants had a 31% reduction in yield with an estimated $440/ha loss of income. Heavily-infected 'Wyldewood' canes had a significant loss in berry yield (47%) and potential income ($2,295/ha), assuming a conservative estimate of five canes/plan. In another study, Colletotrichum was isolated from elderberry leaf spot lesions and identified before subsequent re-inoculation of elderberry plants with this pathogen. Three species of Colletotrichum (C. salicis Funkel, C. kahawae subsp. ciggaro Wollenw., and C. aenigma C.M. Tian and Z. Li) were putatively identified as being casual agents of leaf spot indicating the diversity of species within this genus on elderberry plants.

scholarly journals SEMI-HYDROPONIC CULTIVATION AS A SELECTION TOOL FOR ALUMINUM TOLERANCE IN FORAGE GRASSES

2016 ◽  
Vol 24 (4) ◽  
pp. 350-356
Author(s):  
Gustavo André Colombo ◽  
Aurélio Vaz-de-Melo ◽  
Gilberto Coutinho Machado Filho ◽  
André Silva de Sousa
Keyword(s):  
Nutrient Solution ◽  
Aluminum Toxicity ◽  
Aluminum Tolerance ◽  
Dry Weight ◽  
Panicum Maximum ◽  
Forage Grasses ◽  
Breeding Programs ◽  
Brachiaria Humidicola ◽  
Hydroponic Cultivation ◽  
Constant Improvement

The constant improvement of selection methods is necessary in order to optimize efficiency of breeding programs for aluminum tolerance. Thus, the aim of this study was to establish a vase volume to the ideal semi-hydroponic cultivation of forage grasses subjected to stress by aluminum in nutrient solution. The experimental design completely randomized, with four replicates, each replicate consisting of one plant per vase. The treatments were arranged in a factorial design 4x5, being the first factor consists of four genotypes of forage grasses Brachiaria Humidicola; Brachiaria brizantha cv. Piatã; Panicum maximum cv. Massai and Panicum maximum cv. Mombaça) and the second consisting of five separate volumes plastics vases where seedlings were grown (0.2; 0.25; 0.3; 0.35 and 0.4 dm³). The grasses grown in a semi-hydroponics system irrigated with nutrient solution rich in aluminum (3 mg L-1). Was measured plant height, dry weight of aereal part and root length. The semi-hydroponic cultivation in aluminum increased nutrient solution is effective in differentiation of forage grasses genotypes in relation to aluminum tolerance. Pots volumes near 0.3 dm3 promote greater development for root and aerial part attributes in forage grasses grown in nutrient solution with aluminum toxicity.

scholarly journals AMINO ACID COMPOSITION OF LEAVES OF THREE SPECIES OF ARTEMISIA L. GROWING IN THE ELTON REGION

2021 ◽  
pp. 219-225
Author(s):  
Галина Табаленкова
Keyword(s):  
Amino Acids ◽  
Czech Republic ◽  
Amino Acid ◽  
Dry Weight ◽  
Biologically Active ◽  
Raw Material ◽  
Buffer System ◽  
Amino Acid Analyzer ◽  
Growing Conditions ◽  
High Level

The results of studies of the qualitative composition and quantitative content of amino acids (AAs) in the leaves of three plant species of the genus Artemisia, widespread in the Elton region, were presented. Protein AAs were determined on an AAA T-339 amino acid analyzer (Czech Republic) after hydrolysis of a sample in 6N HCl at 105 °C for 24 h, free AAs – on an AAA-400 amino acid analyzer (Czech Republic) in a lithium buffer system. The protein AAs amount varied from 66 mg / g in A. lerchiana to 113 mg / g dry weight in A. santonica. 17 AAs were found in composition of these species, aspartic and glutamic acids were dominant. The content of free AAs varied from 4.4 mg / g in A. santonica to 8.3 mg / g dry weight in A. pauciflora. 14 AAs have been identified, among them proline was the predominant free AA. The share of proline was 75-81% of the total free AAs. Among the minor components, 3-4 compounds with a content above 2% dominated. The free AAs contain 3 non-proteinogenic ones (ornithine, citruline, and γ-aminobutyric acid). A. lerchiana and A. pauciflora species were similar in protein and free amino acids, probably due to the same growing conditions. A high level of free proline, together with a complex of biologically active substances in Artemisia species, which grow abundantly in the Elton region, allow to consider the possibility of their use as a medicinal raw material.

scholarly journals The Genetic Variability of Sicilian Lemon Germplasm Revealed by Molecular Marker Fingerprints

2008 ◽  
Vol 133 (2) ◽  
pp. 242-248 ◽  
Author(s):  
Mirko Siragusa ◽  
Fabio De Pasquale ◽  
Loredana Abbate ◽  
Letizia Martorana ◽  
Nicasio Tusa
Keyword(s):  
Dna Markers ◽  
Random Amplified Polymorphic Dna ◽  
Citrus Limon ◽  
Genetic Isolation ◽  
Breeding Programs ◽  
Bud Mutation ◽  
Italian Regions ◽  
Wide Range ◽  
High Level ◽  
Polymorphic Nature

There is a high level of diversity among lemons [Citrus limon (L.) Burm. f. (2n = 2x = 18)] in Sicily, where each growing area has a wide range of landraces mostly derived from bud mutation. Because this variability represents an important resource for future breeding programs and genetic improvement, the relationships among the principal 36 accessions of Sicilian lemon, belonging to three different cultivars (Femminello, Monachello, and Lunario), were examined by intersimple sequence repeat and random amplified polymorphic DNA markers. Three ‘Femminello’ accessions from nearby Italian regions were also examined to study the genetic flow from the continent. The disputed case of the accession ‘Eureka Messina lemon’ was also examined, using ‘Frost Eureka’ as a control. Our results confirmed the extreme polymorphic nature of the three principal Sicilian cultivars and the presence of a wide range of different genotypes. Twenty-two Sicilian genotypes were recognized as unique accessions, reflecting the richness of the lemon germplasm present in Sicily. Each growing area showed the presence of several genetically different landraces, probably preserved by genetic isolation, whereas the continental accessions appeared extremely similar to the island genotypes, showing an exchange of germplasm from the island to the continent.

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