2,4-D–Resistant Buckhorn Plantain (Plantago lanceolata) in Managed Turf

2018 ◽  
Vol 32 (2) ◽  
pp. 182-189 ◽  
Author(s):  
Aaron J. Patton ◽  
Daniel V. Weisenberger ◽  
Geoff P. Schortgen
Keyword(s):  
Dose Response ◽  
Plantago Lanceolata ◽  
Resistance Mechanism ◽  
Cross Resistance ◽  
Pyridinecarboxylic Acid ◽  
First Report ◽  
Synthetic Auxin ◽  
Group 4 ◽  
Auxin Herbicides ◽  
Acid Herbicides

AbstractA population of buckhorn plantain with suspected resistance to 2,4-D was identified in central Indiana following 30 yr of 2,4-D–containing herbicide applications. Our objectives were to (1) confirm and quantify the level of herbicide resistance in the buckhorn plantain population using dose–response experiments and (2) find alternative herbicides that could be used to control this population. Greenhouse experiments were conducted to quantify the dose–response of resistant (R) and susceptible (S) biotypes of buckhorn plantain to both 2,4-D and triclopyr, two synthetic auxin herbicides from different chemical families. The R biotype was ≥6.2 times less sensitive to 2,4-D than the S biotype. The efficacy of triclopyr was similar on both the R and S biotypes of buckhorn plantain, suggesting the absence of cross-resistance to this herbicide. This is the first report of 2,4-D resistance in buckhorn plantain and the first report of 2,4-D resistance in turf. The resistance mechanism was limited to within a chemical family (phenoxycarboxylic acid) and did not occur across all WSSA Group 4 synthetic auxin herbicides, as the pyridinecarboxylic acid herbicides clopyralid and triclopyr and the arylpicolinate herbicide halauxifen-methyl provided control in our experiments.

Buckhorn plantain (Plantago lanceolata) resistant to 2,4-D in Pennsylvania and alternative control options

2020 ◽  
pp. 1-7
Author(s):  
Travis R. Russell ◽  
Tim T. Lulis ◽  
Brian A. Aynardi ◽  
Kaiyuan T. Tang ◽  
John E. Kaminski
Keyword(s):  
Herbicide Resistance ◽  
Dose Response ◽  
Field Experiments ◽  
Plantago Lanceolata ◽  
Application Rate ◽  
Field Resistance ◽  
Cross Resistance ◽  
Weed Species ◽  
Synthetic Auxin ◽  
Susceptible Populations

Abstract Buckhorn plantain populations purportedly resistant to 2,4-D were identified in Pennsylvania following long-term, continual applications of the active ingredient to turfgrass. The research objectives of this study were to 1) confirm 2,4-D resistance with dose-response experiments, 2) confirm field resistance of buckhorn plantain to 2,4-D in Pennsylvania, and 3) evaluate alternative herbicides for 2,4-D-resistant buckhorn plantain. Greenhouse dose-response experiments evaluated the sensitivity of buckhorn plantain biotypes that were resistant or susceptible to 2,4-D, and to halauxifen-methyl, two synthetic auxin herbicides from different chemical families. The resistant biotype was ≥11.3 times less sensitive to 2,4-D than the susceptible biotype and required a 2,4-D dosage ≥4.2 times greater than the standard application rate to reach 50% necrosis. No cross-resistance was observed to halauxifen-methyl because both resistant and susceptible populations demonstrated similar herbicide sensitivity. Field experiments confirmed previous reports of ineffectiveness (≤30% reduction) with 2,4-D and other phenoxycarboxylic herbicides in potentially resistant buckhorn plantain biotypes. Treatments containing halauxifen-methyl resulted in a ≥70% reduction in resistant biotypes. This is the first known report of synthetic auxin herbicide resistance in any weed species in Pennsylvania and highlights emerging herbicide resistance challenges in turfgrass systems.

The Influence of Hard Water on 2,4-D Formulations for the Control of Dandelion

2020 ◽  
pp. 1-31
Author(s):  
Geoffrey P. Schortgen ◽  
Aaron J. Patton
Keyword(s):  
Cropping Systems ◽  
Hard Water ◽  
Water Soluble ◽  
Soluble Salt ◽  
Synthetic Auxin ◽  
Auxin Herbicides ◽  
Acid Herbicides

The herbicide 2,4-D is used in a variety of cropping systems, especially in grasses since it is a selective postemergence broadleaf herbicide. However, the most common formulation (2,4-D dimethylamine) is antagonized when mixed in hard water. The objective of this research was to determine which formulations of 2,4-D or premixes of various formulations of synthetic auxin herbicides are subject to hard water antagonism. Formulations surveyed for hard water antagonism in the first experiment included 2,4-D dimethylamine, 2,4-D diethanolamine, 2,4-D monomethylamine, 2,4-D isopropylamine salt, 2,4-D choline salt, 2,4-D isooctyl ester, and 2,4-D ethylhexyl ester. Synthetic auxin formulation types in the second experiment included water-soluble, emulsifiable concentrates and emulsion-in-water. All formulations were mixed with both soft and hard water (600 mg CaCO3 L-1) and applied to dandelions to determine if antagonism occurred in hard water. Water-soluble (amine and choline) 2,4-D formulations were antagonized by hard water, but water-insoluble (ester) 2,4-D formulations were not antagonized. Similar results were found by formulation type with water-soluble synthetic auxin premixes antagonized but emulsifiable concentrates not antagonized. Further, water-soluble salt formulations were not antagonized when formulated in premixes with other synthetic auxin herbicides as an emulsion-in-water. This research demonstrates that all 2,4-D water-soluble formulations and water-soluble premixes with phenoxycarboxylic acid herbicides are subject to hard water antagonism. Formulations of 2,4-D containing emulsifying agents protect against antagonism by the water-insoluble nature of ingredients in their formulation.

scholarly journals Confirmation of S-metolachlor resistance in Palmer amaranth (Amaranthus palmeri)

2019 ◽  
Vol 33 (5) ◽  
pp. 720-726 ◽  
Author(s):  
Chad Brabham ◽  
Jason K. Norsworthy ◽  
Michael M. Houston ◽  
Vijay K Varanasi ◽  
Tom Barber
Keyword(s):  
Dose Response ◽  
Resistance Mechanism ◽  
Acetolactate Synthase ◽  
Chain Fatty Acid ◽  
Palmer Amaranth ◽  
Cross Resistance ◽  
Amaranthus Palmeri ◽  
Glutathione S Transferase ◽  
Long Chain Fatty Acid ◽  
Long Chain

AbstractS-Metolachlor is commonly used by soybean and cotton growers, especially with POST treatments for overlapping residuals, to obtain season-long control of glyphosate- and acetolactate synthase (ALS)–resistant Palmer amaranth. In Crittenden County, AR, reports of Palmer amaranth escapes following S-metolachlor treatment were first noted at field sites near Crawfordsville and Marion in 2016. Field and greenhouse experiments were conducted to confirm S-metolachlor resistance and to test for cross-resistance to other very-long-chain fatty acid (VLCFA)–inhibiting herbicides in Palmer amaranth accessions from Crawfordsville and Marion. Palmer amaranth control in the field (soil <3% organic matter) 14 d after treatment (DAT) was ≥94% with a 1× rate of acetochlor (1,472 g ai ha–1; emulsifiable concentrate formulation) and dimethenamid-P (631 g ai ha–1). However, S-metolachlor at 1,064 g ai ha–1 provided only 76% control, which was not significantly different from the 1/2× and 1/4× rates of dimethenamid-P and acetochlor (66% to 85%). In the greenhouse, Palmer amaranth accessions from Marion and Crawfordsville were 9.8 and 8.3 times more resistant to S-metolachlor compared with two susceptible accessions based on LD50 values obtained from dose–response experiments. Two-thirds and 1.5 times S-metolachlor at 1,064 g ha–1 were the estimated rates required to obtain 90% mortality of the Crawfordsville and Marion accessions, respectively. Data collected from the field and greenhouse confirm that these accessions have evolved a low level of resistance to S-metolachlor. In an agar-based assay, the level of resistance in the Marion accession was significantly reduced in the presence of a glutathione S-transferase (GST) inhibitor, suggesting that GSTs are the probable resistance mechanism. With respect to other VLCFA-inhibiting herbicides, Marion and Crawfordsville accessions were not cross-resistant to acetochlor, dimethenamid-P, or pyroxasulfone. However, both accessions, based on LD50 values obtained from greenhouse dose–response experiments, exhibited reduced sensitivity (1.5- to 3.6-fold) to the tested VLCFA-inhibiting herbicides.

Herbicides for Postemergence Control of Mile-a-Minute (Mikania micrantha)

2014 ◽  
Vol 7 (2) ◽  
pp. 303-309 ◽  
Author(s):  
Brent A. Sellers ◽  
Sarah R. Lancaster ◽  
Kenneth A. Langeland
Keyword(s):  
Dose Response ◽  
South Florida ◽  
Growth Reduction ◽  
Mikania Micrantha ◽  
Synthetic Auxin ◽  
Greenhouse Experiments ◽  
Auxin Herbicides ◽  
Dose Response Study

AbstractGreenhouse experiments were conducted to evaluate mile-a-minute response to selected herbicides. In the first experiment, mile-a-minute response was evaluated following the application of aminocyclopyrachlor, aminopyralid, fluroxypyr, glufosinate, glyphosate, imazamox, imazapic, metsulfuron, penoxsulam, and triclopyr. Applications of aminocyclopyrachlor, aminopyralid, fluroxypyr, glufosinate, glyphosate, and triclopyr all resulted in mile-a-minute control 70% or greater 8 wk after treatment (WAT). Mile-a-minute sensitivity to these six herbicides was further evaluated in a dose-response study. Although 90% growth reduction (GR90) values were determined for aminopyralid and glyphosate, statistically significant results were not obtained for aminocyclopyrachlor, fluroxypyr, glufosinate, and triclopyr. The results of these experiments suggest that glyphosate, as well as the synthetic auxin herbicides aminocyclopyrachlor, aminopyralid, fluroxypyr, and triclopyr, should be further evaluated for mile-a-minute control in south Florida.

scholarly journals Point Mutations as Main Resistance Mechanism Together With P450-Based Metabolism Confer Broad Resistance to Different ALS-Inhibiting Herbicides in Glebionis coronaria From Tunisia

2021 ◽  
Vol 12 ◽  
Author(s):  
Zeineb Hada ◽  
Yosra Menchari ◽  
Antonia M. Rojano-Delgado ◽  
Joel Torra ◽  
Julio Menéndez ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Dose Response ◽  
Resistance Mechanism ◽  
Point Mutations ◽  
Acetolactate Synthase ◽  
Target Site ◽  
Cross Resistance ◽  
P450 Monooxygenase ◽  
Target Site Resistance ◽  
Sites Of Action

Resistance to acetolactate synthase (ALS) inhibiting herbicides has recently been reported in Glebionis coronaria from wheat fields in northern Tunisia, where the weed is widespread. However, potential resistance mechanisms conferring resistance in these populations are unknown. The aim of this research was to study target-site resistance (TSR) and non-target-site resistance (NTSR) mechanisms present in two putative resistant (R) populations. Dose–response experiments, ALS enzyme activity assays, ALS gene sequencing, absorption and translocation experiments with radiolabeled herbicides, and metabolism experiments were carried out for this purpose. Whole plant trials confirmed high resistance levels to tribenuron and cross-resistance to florasulam and imazamox. ALS enzyme activity further confirmed cross-resistance to these three herbicides and also to bispyribac, but not to flucarbazone. Sequence analysis revealed the presence of amino acid substitutions in positions 197, 376, and 574 of the target enzyme. Among the NTSR mechanisms investigated, absorption or translocation did not contribute to resistance, while evidences of the presence of enhanced metabolism were provided. A pretreatment with the cytochrome P450 monooxygenase (P450) inhibitor malathion partially synergized with imazamox in post-emergence but not with tribenuron in dose–response experiments. Additionally, an imazamox hydroxyl metabolite was detected in both R populations in metabolism experiments, which disappeared with the pretreatment with malathion. This study confirms the evolution of cross-resistance to ALS inhibiting herbicides in G. coronaria from Tunisia through TSR and NTSR mechanisms. The presence of enhanced metabolism involving P450 is threatening the chemical management of this weed in Tunisian wheat fields, since it might confer cross-resistance to other sites of action.

scholarly journals Patterns of resistance to AHAS inhibitors in Limnocharis flava from Malaysia

2017 ◽  
Vol 54 (No. 1) ◽  
pp. 48-59
Author(s):  
Zakaria Norazua ◽  
Ahmad-Hamdani Muhammad Saiful ◽  
Juraimi Abdul Shukor
Keyword(s):  
Dose Response ◽  
Resistance Mechanisms ◽  
Rice Fields ◽  
Cross Resistance ◽  
Multiple Resistance ◽  
Low Level ◽  
Metsulfuron Methyl ◽  
Synthetic Auxin ◽  
Target Site Resistance ◽  
High Level

Limnocharis flava (L.) Buchenau is among the most problematic rice weeds in Malaysia and is also reported to have developed multiple resistance to AHAS inhibitor bensulfuron-methyl and synthetic auxin 2,4-D. In this study, resistance across different AHAS inhibitors was characterised in a L. flava population infesting rice fields in Pulau Pinang, Malaysia. Dose-response experiments were conducted to determine the level of resistance to sulfonylureas, imidazolinone, triazolopyrimidine, and pyrimidinyl-thiobenzoate. Cross-resistance across different AHAS inhibitors was observed in the resistant L. flava population, exhibiting a high level of resistance to bensulfuron-methyl, while exhibiting a moderate level of resistance to metsulfuron-methyl and a low level of resistance to pyrazosulfuron-ethyl and pyribenzoxim. However, all resistant L. flava individuals were still sensitive to imazethapyr, penoxsulam, and bispyribac-sodium. Based on the results, it is likely that resistance to AHAS inhibitors in L. flava is conferred by target-site resistance mechanisms.

Herbicide diagnostics reveal multiple patterns of synthetic auxin resistance in kochia (Bassia scoparia)

2021 ◽  
pp. 1-28
Author(s):  
Charles M. Geddes ◽  
Mallory L. Owen ◽  
Teandra E. Ostendorf ◽  
Julia Y. Leeson ◽  
Shaun M. Sharpe ◽  
...  
Keyword(s):  
Great Plains ◽  
Dose Response ◽  
Weed Management ◽  
Acetolactate Synthase ◽  
The United States ◽  
Cross Resistance ◽  
Visible Injury ◽  
Synthetic Auxin ◽  
Auxin Resistance ◽  
Sites Of Action

Abstract Herbicide-resistant (HR) kochia is a growing problem in the Great Plains region of Canada and the United States (U.S.). Resistance to up to four herbicide sites of action, including photosystem II inhibitors, acetolactate synthase inhibitors, synthetic auxins, and the 5-enolpyruvylshikimate-3-phosphate synthase inhibitor glyphosate have been reported in many areas of this region. Despite being present in the U.S. since 1993/1994, auxinic-HR kochia is a recent and growing phenomenon in Canada. This study was designed to characterize (a) the level of resistance and (b) patterns of cross-resistance to dicamba and fluroxypyr in 12 putative auxinic-HR kochia populations from western Canada. The incidence of dicamba-resistant individuals ranged among populations from 0% to 85%, while fluroxypyr-resistant individuals ranged from 0% to 45%. In whole-plant dose-response bioassays, the populations exhibited up to 6.5-fold resistance to dicamba and up to 51.5-fold resistance to fluroxypyr based on visible injury 28 days after application. Based on plant survival estimates, the populations exhibited up to 3.7-fold resistance to dicamba and up to 72.5-fold resistance to fluroxypyr. Multiple patterns of synthetic auxin resistance were observed, where one population from Cypress County, Alberta was resistant to dicamba but not fluroxypyr, while another from Rocky View County, Alberta was resistant to fluroxypyr but not dicamba based on single-dose population screening and dose-response bioassays. These results suggest that multiple mechanisms may confer resistance to dicamba and/or fluroxypyr in Canadian kochia populations. Further research is warranted to determine these mechanisms. Farmers are urged to adopt proactive non-chemical weed management tools in an effort to preserve efficacy of the remaining herbicide options available for control of HR kochia.

Efficacy of triclopyr and synthetic auxin herbicide mixtures for common blue violet (Viola sororia) control

2020 ◽  
Vol 34 (4) ◽  
pp. 475-481
Author(s):  
Aaron J. Patton ◽  
Daniel V. Weisenberger ◽  
Wenwen Liu
Keyword(s):  
Data Collection ◽  
Chlorophyll Content ◽  
Dose Response ◽  
Dry Weight ◽  
Synthetic Auxin ◽  
Comparison Experiment ◽  
Herbicide Mixtures ◽  
Auxin Herbicides ◽  
High Label

AbstractCommon blue violet is a widely distributed, perennial broadleaf that is difficult to control in lawns. Two experiments were conducted to evaluate the efficacy of synthetic auxin herbicides and their mixtures or rate for common blue violet control. A herbicide comparison experiment was conducted with treatments including a nontreated check; 2,4-D dimethylamine; 2,4-D isooctyl ester (2,4-D ester); dichlorprop (2,4-DP) ethylhexyl ester, MCPA dimethylamine; mecoprop dimethylamine; triclopyr butoxyethyl ester; quinclorac; and mixtures of triclopyr + quinclorac; 2,4-D ester + 2,4-DP; 2,4-D ester + triclopyr; 2,4-D ester + 2,4-DP + triclopyr. All herbicides were applied at 1.12 kg ae ha−1 except quinclorac (0.84 kg ha−1). Additionally, a triclopyr dose-response experiment was conducted using rates of 0, 0.14, 0.28, 0.56, 0.84, and 1.12 kg ha−1. Epinasty ranged from 80% to 99% at 21 d after application for triclopyr-containing treatments and no more than 28% for all other treatments. Plant mass from harvest and regrowth data from the comparison experiment indicated triclopyr-containing treatments provided the highest common blue violet control. Mixtures containing triclopyr did not differ from triclopyr alone, indicating there was no added effect between herbicide mixtures. The triclopyr dose-response experiment confirmed triclopyr efficacy across data collection types. As triclopyr dose increased, violet epinasty increased and chlorophyll content and dry weight decreased. Triclopyr applied at 0.81 kg ha−1 or greater concentration provided 75% or greater control, as indicated by regrowth data. Many herbicides containing triclopyr are registered for use in turf, but most apply a concentration not greater than 0.56 kg ha−1 triclopyr when applied at the high label rate. Thus, to achieve good (75%) common blue violet control, turf managers should select products that contain triclopyr and apply doses of at least 0.81 kg ha−1 when used according to the label.

scholarly journals Hormetic Concentrations of Hydrogen Peroxide but Not Ethanol Induce Cross-Adaptation to Different Stresses in Budding Yeast

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Halyna M. Semchyshyn
Keyword(s):  
Hydrogen Peroxide ◽  
Dose Response ◽  
Budding Yeast ◽  
Regulatory Protein ◽  
Colony Growth ◽  
Inhibitory Effect ◽  
Cross Resistance ◽  
Mild Stress ◽  
Low Concentrations ◽  
Cross Adaptation

The biphasic-dose response of microorganisms to hydrogen peroxide is a phenomenon of particular interest in hormesis research. In different animal models, the dose-response curve for ethanol is also nonlinear showing an inhibitory effect at high doses but a stimulatory effect at low doses. In this study, we observed the hormetic-dose response to ethanol in budding yeastS. cerevisiae. Cross-protection is a phenomenon in which exposure to mild stress results in the acquisition of cellular resistance to lethal stress induced by different factors. Since both hydrogen peroxide and ethanol at low concentrations were found to stimulate yeast colony growth, we evaluated the role of one substance in cell cross-adaptation to the other substance as well as some weak organic acid preservatives. This study demonstrates that, unlike ethanol, hydrogen peroxide at hormetic concentrations causes cross-resistance ofS. cerevisiaeto different stresses. The regulatory protein Yap1 plays an important role in the hormetic effects by low concentrations of either hydrogen peroxide or ethanol, and it is involved in the yeast cross-adaptation by low sublethal doses of hydrogen peroxide.

Weed Control with Halauxifen-Methyl Applied Alone and in Mixtures with 2,4-D, Dicamba, and Glyphosate

2018 ◽  
Vol 32 (5) ◽  
pp. 597-602 ◽  
Author(s):  
Marcelo Zimmer ◽  
Bryan G. Young ◽  
William G. Johnson
Keyword(s):  
Weed Control ◽  
Field Experiments ◽  
Common Ragweed ◽  
Synthetic Auxin ◽  
Redroot Pigweed ◽  
Giant Ragweed ◽  
Auxin Herbicides ◽  
Control Spectrum

AbstractSynthetic auxin herbicides such as 2,4-D and dicamba are often utilized to control broadleaf weeds in preplant burndown applications to soybean. Halauxifen-methyl is a new synthetic auxin herbicide for broadleaf weed control in preplant burndown applications to corn, cotton, and soybean at low use rates (5 g ae ha–1). Field experiments were conducted to evaluate efficacy and weed control spectrum of halauxifen-methyl applied alone and in mixtures with 2,4-D (560 g ae ha–1), dicamba (280 g ae ha–1), and glyphosate (560 g ae ha–1). Glyphosate-resistant (GR) horseweed was controlled with halauxifen-methyl applied alone (90% control) and in mixtures (87% to 97% control) 35 d after treatment (DAT). Common ragweed was controlled 93% with halauxifen-methyl applied alone and 91% to 97% in mixtures 35 DAT. Halauxifen-methyl applied alone resulted in poor giant ragweed control 21 DAT (73% control); however, mixtures of halauxifen-methyl with 2,4-D, dicamba, or glyphosate controlled giant ragweed (86% to 98% control). Halauxifen-methyl alone resulted in poor redroot pigweed control (62% control) 21 DAT; however, mixtures of halauxifen-methyl with dicamba, 2,4-D, or glyphosate controlled redroot pigweed (89% to 98% control). Halauxifen-methyl controls GR horseweed and common ragweed applied alone and in mixtures with other synthetic auxin herbicides and glyphosate. Furthermore, mixing 2,4-D or dicamba with halauxifen-methyl can increase the weed control spectrum in preplant burndown applications.

Sign in / Sign up

Export Citation Format

Share Document