scholarly journals 3D Imaging of Dendritic Cells Using Serial Ion-Abrasion Scanning Electron Microscopy (SIASEM) Reveals That HIV-1 is Stored Within Surface-connected Membrane Folds

2010 ◽  
Vol 16 (S2) ◽  
pp. 1098-1099
Author(s):  
N Avishai ◽  
A Avishai ◽  
H Yu ◽  
M Hitomi ◽  
A Heuer ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Dendritic Cells ◽  
3D Imaging ◽  
Hiv 1 ◽  
Scanning Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.

scholarly journals HIV-1 activates Cdc42 and induces membrane extensions in immature dendritic cells to facilitate cell-to-cell virus propagation

Blood ◽  
2011 ◽  
Vol 118 (18) ◽  
pp. 4841-4852 ◽  
Author(s):  
Damjan S. Nikolic ◽  
Martin Lehmann ◽  
Richard Felts ◽  
Eduardo Garcia ◽  
Fabien P. Blanchet ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Dendritic Cells ◽  
Electron Tomography ◽  
Cell Contact ◽  
Virus Propagation ◽  
Immature Dendritic Cells ◽  
Cell Transmission ◽  
Hiv 1 ◽  
Scanning Electron

AbstractHIV-1 cell-to-cell transmission confers a strong advantage as it increases efficiency of transfer up to 100-fold compared with a cell-free route. Mechanisms of HIV-1 cell-to-cell transmission are still unclear and can in part be explained by the presence of actin-containing cellular protrusions. Such protrusions have been shown to facilitate cell-to-cell viral dissemination. Using fluorescence microscopy, electron tomography, and ion abrasion scanning electron microscopy we show that HIV-1 induces membrane extensions in immature dendritic cells through activation of Cdc42. We demonstrate that these extensions are induced after engagement of DC-SIGN by HIV-1env via a cascade that involves Src kinases, Cdc42, Pak1, and Wasp. Silencing of Cdc42 or treatment with a specific Cdc42 inhibitor, Secramine A, dramatically reduced the number of membrane protrusions visualized on the cell surface and decreased HIV-1 transfer via infectious synapses. Ion abrasion scanning electron microscopy of cell-cell contact regions showed that cellular extensions from immature dendritic cells that have the appearance of thin filopodia in thin section images are indeed extended membranous sheets with a narrow cross section. Our results demonstrate that HIV-1 binding on immature dendritic cells enhances the formation of membrane extensions that facilitate HIV-1 transfer to CD4+ T lymphocytes.

scholarly journals 3D imaging by serial block face scanning electron microscopy for materials science using ultramicrotomy

2016 ◽  
Vol 163 ◽  
pp. 6-18 ◽  
Author(s):  
Teruo Hashimoto ◽  
George E. Thompson ◽  
Xiaorong Zhou ◽  
Philip J. Withers
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
3D Imaging ◽  
Materials Science ◽  
Face Scanning ◽  
Block Face ◽  
Scanning Electron

scholarly journals 3D Imaging of Diatoms with Ion Abrasion Scanning Electron Microscopy

2008 ◽  
Vol 14 (S2) ◽  
pp. 972-973
Author(s):  
M Hildebrand ◽  
S Kim ◽  
D Shi ◽  
S Subramaniam
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Mexico ◽  
3D Imaging ◽  
Scanning Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008

Fine structure of dendritic cells in the epithelial cell layer of the ox tongue

1990 ◽  
Vol 48 (3) ◽  
pp. 528-529
Author(s):  
Kan Kobayashi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Dendritic Cells ◽  
Epithelial Cell ◽  
Connective Tissue ◽  
Cell Layer ◽  
Transmission Electron ◽  
Epithelial Cell Layer ◽  
Scanning Electron

It is known that some kinds of dendritic cells are distributed in the epithelial cell layer of mucous membrane consisting of stratified squamous epithelium. In the process of exfoliation of the epithelial layer from the underlying connective tissue, dendritic cell bodies exposed on the ruptured surface of the epithelium were detected by scanning electron microscopy. These cells were also observed by light microscopy as well as by transmission electron microscopy.Dorsal mucous membrane of the ox tongue was fixed in Karnovsky's fixative or in 10% formalin. For scanning electron microscopy samples were immersed in 3N-HCl solution for 2-3 weeks at room temperature. The epithelial cell layer was removed from the underlying connective tissue layer1). They were postfixed in tannic acid and then 1% OsO4 for 1 hr. After dehydration in an ethanol series, the specimens were dried by passing through t-butylalcohol, coated with platinum-palladium and observed under an S-800 scanning electron microscope. For transmission electron microscopy, small pieces of the fixed tissue were post-fixed in 1% OsO4 for 1.5 hr and then embedded in Araldite-Epon.

scholarly journals 3D imaging of diatoms with ion-abrasion scanning electron microscopy

2009 ◽  
Vol 166 (3) ◽  
pp. 316-328 ◽  
Author(s):  
Mark Hildebrand ◽  
Sang Kim ◽  
Dan Shi ◽  
Keana Scott ◽  
Sriram Subramaniam
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
3D Imaging ◽  
Scanning Electron

3D imaging of full-term human placental villi by serial block face-scanning electron microscopy

Placenta ◽  
2017 ◽  
Vol 59 ◽  
pp. 175-176
Author(s):  
Haruhiko Shimada ◽  
Yuuki Yamaguchi ◽  
Takami Takizawa ◽  
Hironori Takahashi ◽  
Akihid Ohkuchi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
3D Imaging ◽  
Full Term ◽  
Face Scanning ◽  
Placental Villi ◽  
Block Face ◽  
Scanning Electron
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