Striated Muscle in the Intestine of Shrimp

1988 ◽  
Vol 46 ◽  
pp. 266-267
Author(s):  
K. C. Liu ◽  
S. F. Tsay ◽  
Y. L. Yu
Keyword(s):  
Striated Muscle ◽  
Penaeus Monodon ◽  
Grass Shrimp ◽  
Circular Muscle ◽  
Electron Microscopic Observation ◽  
Muscularis Mucosa ◽  
Structural Element ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Similar Report

The shrimp, Penaeus monodon, commonly known as grass shrimp by local people. It has been cultured in large quantity and became one of the important aqua- cultural species in recent years. The morphology and histology of penaeid shrimps has been studied by many researchers (1). In our pathological study of the structural aspects of the digestive system, striated muscle was found in the area equivalent to the muscularis mucosa of tunica mucosa and tun.ica muscularis of vertebrates where smooth muscle is the basic structural element. This information is new to us , and to the best of our knowledge, there is no similar report among other Crustacea (2). This report is to present some of our evidence.Shrimps, Penaeus monodon, 50 g. in average body weight, were bought live from the market. Their intestines were disssected out and processed for both light electron microscopic studies. For electron microscopic observation a JEOL 100 CX electron microscope was used at 60 KV. attention was primarily concentrated on the circular muscle of the muscularis mucosa region.

scholarly journals THE RELATIONSHIP BETWEEN MYOFILAMENT PACKING DENSITY AND SARCOMERE LENGTH IN FROG STRIATED MUSCLE

1967 ◽  
Vol 33 (2) ◽  
pp. 255-263 ◽  
Author(s):  
Philip W. Brandt ◽  
Enrique Lopez ◽  
John P. Reuben ◽  
Harry Grundfest
Keyword(s):  
Cross Sections ◽  
Packing Density ◽  
Sarcomere Length ◽  
Striated Muscle ◽  
X Ray Diffraction ◽  
Electron Microscopic ◽  
Semitendinosus Muscle ◽  
Direct Function ◽  
Microscopic Studies

In cross-sections of single fibers from the frog semitendinosus muscle the number of thick myofilaments per unit area (packing density) is a direct function of the sarcomere length. Our data, derived from electron microscopic studies, fit well with other data derived from in vivo, low-angle X-ray diffraction studies of whole semitendinosus muscles. The data are consistent with the assumption that the sarcomere of a fibril maintains a constant volume during changes in sarcomere length. The myofilament lattice, therefore, expands as the sarcomere shortens. Since the distance between adjacent myofilaments is an inverse square root function of sarcomere length, the interaction of the thick and the thin myofilaments during sarcomere shortening may occur over distances which increase 70 A or more. The "expanding-sarcomere, sliding-filament" model of sarcomere shortening is discussed in terms of the current concepts of muscle architecture and contraction.

A HISTOCHEMICAL AND ELECTRON MICROSCOPIC STUDY OF SKELETAL MUSCLE IN A CASE OF POMPE'S DISEASE (GLYCOGENOSIS II)

PEDIATRICS ◽  
1966 ◽  
Vol 37 (2) ◽  
pp. 249-259
Author(s):  
Robert Darrell Cardiff
Keyword(s):  
Skeletal Muscle ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Striated Muscle ◽  
Electron Microscopic ◽  
Pompe’S Disease ◽  
Pompe's Disease ◽  
Microscopic Studies ◽  
Alpha Glucosidase ◽  
Glycogenosis Ii

1. A case of Pompe's disease (glycogenosis II) without biochemically or histochemically demonstrable alpha-glucosidase activity is described. 2. Histochemical studies of skeletal muscle suggested that the glycogen is frequently stored as an acid mucopolysaccharide. 3. Electron microscopic studies revealed that the major glycogen deposits in most tissue were within membrane-limited sacs. Striated muscle was an exception because major deposits were frequently extrasaccular. 4. The findings in this case are discussed in relation to current concepts of Pompe's disease. In view of the extrasaccular glycogen deposits in skeletal muscle, it is suggested that an extralysosomal factor plays a significant role in the pathogenesis of Pompe's disease.

scholarly journals THE FINE STRUCTURE OF STRIATED MUSCLE

1956 ◽  
Vol 2 (4) ◽  
pp. 131-142 ◽  
Author(s):  
A. J. Hodge
Keyword(s):  
Skeletal Muscle ◽  
Phase Contrast ◽  
Striated Muscle ◽  
General Structure ◽  
Electron Microscopic ◽  
Strong Contraction ◽  
Other Substances ◽  
Microscopic Studies ◽  
Insect Skeletal Muscle ◽  
Band Material

The available evidence from phase contrast, polarization optical, and electron microscopic studies on vertebrate skeletal muscle, insect skeletal muscle, and dipteran flight muscle is interpreted as favoring the following general structure of striated muscle. A continuous array of filaments (actin) runs through all bands of the sarcomere. These are linked by an axially periodic system of transverse filamentous bridges. Myosin (and probably other substances) are localized in the A bands. The system of transverse bridges compensates the birefringence of actin and is thus responsible for the isotropy of the I band. Myosin is responsible for the birefringence of the A bands. On strong contraction, A band material migrates to the Z bands to form contraction bands. It is not yet certain whether this migration involves myosin or another A band component.

Immuno and histochemical electron microscopic studies of the selective permeability, involving the feto-maternal junctional barrier

1978 ◽  
Vol 36 (2) ◽  
pp. 542-543
Author(s):  
Toshio Hata ◽  
Kimiyasu Ohkawa
Keyword(s):  
Electron Microscopic Observation ◽  
Deficiency Anemia ◽  
Surgical Removal ◽  
Electron Microscopic ◽  
Absorption Mechanism ◽  
Junctional Zone ◽  
Selective Permeability ◽  
Transmission Electron ◽  
Microscopic Studies

Regarding to the cellular transfer and absorption mechanism of the trophoblast in the feto-maternal junctional zone, it is awaited futher confirmation in the future. Numerous documentation has been accumulated on this aspect, utilizing the visible markers such as the fat(Yamaguchi), ferritin(Tillack) and IgG(Morphis). however, the observations are mainly based on the investigations of the placenta in vitro. From this view point, the authors have made a rather comprehensive observation of the cellular transfer and absorption mechanism of the human feto-maternal junction in physiological circumstance.Materials and methods: The specimens of the human uterus in early ges tation are removed surgically for therapeutic purpose had served as materials. The chorionic tissue and implanted tissue from the uterus were carefully investigated. The method of the investigation are as follows: 1) Conventional transmission electron microscopic observation with double electron stains. 2) Visualization of the iron dextran(Fesin), it is usually used to iron deficiency anemia, which was administered prior to the surgical removal of the uterus.

Electron microscopic study of effects of Polyactin A on the colonies cells of spleen

1990 ◽  
Vol 48 (3) ◽  
pp. 286-287
Author(s):  
Zeng Xiang Yuan ◽  
Zhang Ying
Keyword(s):  
Stem Cells ◽  
Aplastic Anemia ◽  
Clinical Observation ◽  
Electron Microscopic Observation ◽  
Hemopoietic Stem Cell ◽  
Good Effect ◽  
Electron Microscopic ◽  
Hemolytic Streptococcus ◽  
Microscopic Studies ◽  
First Time

Electron microscopic studies on the colonies cells of spleen were presented.Polyactin A is a new immunological enhancement agent which was developped in China for the first time. It is a polysaccharide extracted from cultured a-hemolytic streptococcus No. 33 in the mouth. Clinical observation suggests that the drug has marked inhibiting effects on some tumors and can increase the number of leucocytes, enhance immunity of the organism. Polyactin A has also a good effect on aplastic anemia and it is a new drug in treatment of aplastic anemia. However, its mechanism isn't understand.In order to investigation this problem, electron microscopic observation on the colonies cells of spleen (CFU-s) were studied and effects of Polyactin A on hemopoietic stem cell of marrow were also discussed. The results showed that Polyactin A can make marked effects on ultrastructures of colonies cells of spleen (CFU-s). Stem cells dilated and nucieus showed inregularity in shape. Chromatin in the nucleus which has enlarged nucleolus was compact and euchromatin increased. There were abundant organelles such as rough endoretigrum, ribsomes and mitochondria in the cytoplasma.

The location of chloride in single striated muscle fibers of the giant barnacle

1968 ◽  
Vol 46 (2) ◽  
pp. 213-219 ◽  
Author(s):  
D. C. Gayton ◽  
J. A. M. Hinke
Keyword(s):  
Membrane Potential ◽  
Striated Muscle ◽  
Total Concentration ◽  
Large Fraction ◽  
Muscle Fibers ◽  
Large Drop ◽  
Fiber Volume ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Striated Muscle Fibers

Chloride-sensitive Ag–AgCl microelectrodes were inserted into single striated muscle fibers of the giant barnacle, Balanus nubilus, to measure the activity of Cl− in the myoplasm, (aCl)i. Chemical analysis was also carried out to determine the total concentration of Cl− in the fiber, [Cl]i. In two sets of experiments, (aCl)i was 28.8 and 22.4 mM while [Cl]i was 75.1 and 66.8 mmoles/kg fiber water respectively. The transmembrane Cl− potential, calculated from the aCl measurements in the myoplasm and the bath, was slightly less than the membrane potential. To locate the large fraction of fiber Cl− that is not free in the myoplasm, Cl− washout studies were done in constant [K]o[Cl]o product Ringer solutions in which [Cl]o was reduced to 50% and 25% of the normal concentration. Fibers which were soaked in these solutions for 30 min showed no change in (aCl)i but a large drop in [Cl]i. From the extent of this drop, it was calculated that these muscle fibers have an extracellular space of about 5% of fiber volume. Electron microscopic studies indicate that this space is comprised of large clefts and smaller tubules which penetrate deeply into the fiber.

scholarly journals Scanning Electron Microscopic Studies on Uterus of Jaffarabadi Buffalo during Follicular and Luteal Phases of Estrous Cycle

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
Vishnudeo Kumar ◽  
K. N. Vyas ◽  
P. H. Tank ◽  
Anil Sharma ◽  
S. H. Talekar
Keyword(s):  
Luteal Phase ◽  
Electron Microscopic Observation ◽  
Hexagonal Structure ◽  
Follicular Phase ◽  
Secretory Cells ◽  
Ciliated Cells ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic ◽  
Microscopic Studies ◽  
Scanning Electron

The study was conducted on uterus of 20 adult Jaffrabadi buffaloes. Scanning electron microscopic observation showed that the surface of endometrium was folded. Lining epithelium of horn and body consist of ciliated and nonciliated cells. During follicular phase cells were flat, formed hexagonal structure with numerous microvilli, whereas, cells were narrow and polygonal in shape during luteal phase. More number of well-developed ciliated cells were found in follicular phase as compared to luteal phase. Numerous secretory blabs were observed on surface of secretory cells during luteal phase. The folds in the cervix were narrow and deep. The lining epithelium of cervix had ciliated and nonciliated cells. The cilia were more in cranial part than the caudal part of the cervix and present in the form of bunch, which were overlapping the secretory cells. Ciliated cells were more in number during follicular phase. Cervical glands were distributed on the cervical mucosa and ciliated cells were present around the openings of these glands.

scholarly journals Assembly of different isoforms of actin and tropomyosin into the skeletal tropomyosin-enriched microfilaments during differentiation of muscle cells in vitro.

1986 ◽  
Vol 103 (6) ◽  
pp. 2173-2183 ◽  
Author(s):  
J J Lin ◽  
J L Lin
Keyword(s):  
Monoclonal Antibody ◽  
Striated Muscle ◽  
Chicken Embryo Fibroblast ◽  
Muscle Cells ◽  
Electron Microscopic ◽  
Thin Filaments ◽  
Microscopic Studies ◽  
The One ◽  
Muscle Isoforms

We have used a monoclonal antibody (CL2) directed against striated muscle isoforms of tropomyosin to selectively isolate a class of microfilaments (skeletal tropomyosin-enriched microfilaments) from differentiating muscle cells. This class of microfilaments differed from the one (tropomyosin-enriched microfilaments) isolated from the same cells by a monoclonal antibody (LCK16) recognizing all isoforms of muscle and nonmuscle tropomyosin. In myoblasts, the skeletal tropomyosin-enriched microfilaments had a higher content of alpha-actin and phosphorylated isoforms of tropomyosin as compared with the tropomyosin-enriched microfilaments. Moreover, besides muscle isoforms of actin and tropomyosin, significant amounts of nonmuscle isoforms of actin and tropomyosin were found in the skeletal tropomyosin-enriched microfilaments of myoblasts and myotubes. These results suggest that different isoforms of actin and tropomyosin can assemble into the same set of microfilaments, presumably pre-existing microfilaments, to form the skeletal tropomyosin-enriched microfilaments, which will eventually become the thin filaments of myofibrils. Therefore, the skeletal tropomyosin-enriched microfilaments detected here may represent an intermediate class of microfilaments formed during thin filament maturation. Electron microscopic studies of the isolated microfilaments from myoblasts and myotubes showed periodic localization of tropomyosin molecules along the microfilaments. The tropomyosin periodicity in the microfilaments of myoblasts and myotubes was 35 and 37 nm, respectively, whereas the nonmuscle tropomyosin along chicken embryo fibroblast microfilaments had a 34-nm repeat.

Electron microscopic studies on ultrathin frozen sections of lactating mouse mammary gland

1978 ◽  
Vol 36 (2) ◽  
pp. 46-47
Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder
Keyword(s):  
Mammary Gland ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Freeze Substitution ◽  
Electron Microscopic Examination ◽  
Mouse Mammary Gland ◽  
Electron Microscopic ◽  
Preparation Methods ◽  
Microscopic Studies ◽  
Ultrathin Frozen Sections

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.

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