Ultrastructural Evaluation of Ischemic Skeletal Muscle Reperfused with Oxygen Free Radical Scavengers

1988 ◽  
Vol 46 ◽  
pp. 262-263
Author(s):  
A. Mathur ◽  
R.C. Russell ◽  
A.C. Roth ◽  
D. Wagahoff
Keyword(s):  
Skeletal Muscle ◽  
Free Radical ◽  
White Male ◽  
Oxygen Free Radical ◽  
Free Radical Scavenger ◽  
Free Radical Scavengers ◽  
Radical Scavenger ◽  
Radical Scavengers ◽  
Tibialis Muscle ◽  
The Right

Skeletal muscle is very sensitive to ischemia. A prolonged preoperative ischemic interval decreases the chances for successful replantation of an amputated limb. Recent experimental work has implicated toxic oxygen free radicals produced during reperfusion of ischemic tissue to be responsible for some of the observed deleterious cellular changes. Selected oxygen free radical scavengers have been used to increase skeletal muscle survival following total ischemia. This study examined the effect of systemic administration of a superoxide free radical scavenger, superoxide dismutase (SOD), and a hydroxyl radical scavenger, dimethylsulfoxide (DMSO) just prior to reperfusion, on the ultrastructure and function of ischemic rabbit anterior tibialis muscle.White male New Zealand rabbits were used for this study. The right hind limb of each rabbit was made ischemic by dissecting and clamping the femoral artery and vein proximal to the bifurcation of the profundus artery and vein. A penrose tourniquet was also placed around the knee. The animals were divided into groups subjected to either 5 or 8 hours of ischemia.

Experimental Evaluation of Oxygen Free Radical Scavengers in the Prevention of Reperfusion Injury to Skeletal Muscle

1989 ◽  
Vol 22 (4) ◽  
pp. 321-331 ◽  
Author(s):  
Axel Mario Feller ◽  
Allan C. Roth ◽  
Robert C. Russell ◽  
Bernard Eagleton ◽  
Hans Suchy ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Free Radical ◽  
Reperfusion Injury ◽  
Experimental Evaluation ◽  
Oxygen Free Radical ◽  
Free Radical Scavengers ◽  
Radical Scavengers

Experimental Evaluation of Oxygen-free Radical Scavengers in the Prevention of Reperfusion Injury to Skeletal Muscle

1988 ◽  
Vol 21 (5) ◽  
pp. 500
Author(s):  
Axel Mario Feller ◽  
Allan C. Roth ◽  
Robert C. Russell ◽  
Bernard Eagleton ◽  
Hans Suchy ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Free Radical ◽  
Reperfusion Injury ◽  
Experimental Evaluation ◽  
Oxygen Free Radical ◽  
Free Radical Scavengers ◽  
Radical Scavengers

Free Radical Scavengers, Antioxidants and Aldose Reductase Inhibitors from Camptosorus sibiricus Rupr.

2008 ◽  
Vol 63 (1-2) ◽  
pp. 66-68 ◽  
Author(s):  
Ning Li ◽  
Xian Li ◽  
Yi Zhang ◽  
Tao Wang ◽  
Wan Xiao
Keyword(s):  
Free Radical ◽  
Aldose Reductase ◽  
Free Radical Scavenger ◽  
Free Radical Scavengers ◽  
Radical Scavenger ◽  
Scavenging Activity ◽  
Radical Scavengers ◽  
Reductase Inhibitors ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Flavonoids and organic acids were recommended in the literature as the main active constituents of Camptosorus sibiricus Rupr. Assay-guided fractionation led to the isolation of 9 flavonoids and 8 phenolic acids. All compounds were tested for DPPH scavenging activity, SOD-like and aldose reductase inhibition. Among them, compounds 1, 2, 3, 5, 6, 7, 8, 9, 11, 15 showed activities. The most active free radical scavenger and antioxidant was compound 8, while compound 1 exhibited strong inhibiting activity of aldose reductase. The structureactivity relation was dicussed briefly.

scholarly journals Bioimmunological responses to Schistosoma mansoni and Fasciola gigantica worm homogenates either with or without saponin

2010 ◽  
Vol 4 (05) ◽  
pp. 334-344
Author(s):  
Amany Sayed Maghraby ◽  
Manal Abdel-Aziz Hamed ◽  
Sanaa Ahmed Ali
Keyword(s):  
Free Radical ◽  
Liver Function ◽  
Schistosoma Mansoni ◽  
Subcutaneous Administration ◽  
Free Radical Scavenger ◽  
Fasciola Gigantica ◽  
Free Radical Scavengers ◽  
Radical Scavenger ◽  
Immune Mediated ◽  
Mice Liver

Background: In this study, we evaluated the biochemical, immunological, histopathological and antischistosomal activities of Schistosoma mansoni or Fasciola gigantica worm homogenates mixed either with or without saponin that was extracted from Atriplex nummularia. Methodology: The immunization schedule was based on subcutaneous administration of two doses (50 μg /100 μl PBS) of each homogenate with time intervals of 15 days. After 15 days of the last homogenate inoculation, all mice were challenged with 100 Schistosoma mansoni cercariae and sacrificed after two months. Free radical scavengers and liver function enzymes were determined in mice liver. Worm counting and the histopathological picture of the liver were also done. Results: Immunization with Schistosoma or Fasciola worm homogenates, mixed either with or without saponin, recorded an amelioration of the free radical scavenger levels, liver function enzymes and reduction in worm burden, as well as improvement of the histological feature of the liver, the number and size of granuloma, evidence of increased immune reaction manifested by a lymphocytic cuff surrounding the granuloma, diminution of its fibrotic and collagen content, and destruction of Schistosoma ova. Conclusion: Fasciola or Schistosoma worm antigens mixed with or without saponin succeeded to eliminate the product of oxidative stress and assistance in immune-mediated destruction of eggs that ameliorate the histopathological picture of the liver cells and preserve its function.

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