An Electron Histochemical and Ultrastructural Evaluation of Human Nasal Septal Cartilage Grafts Stored in Merthiolate® and Alcide®

1988 ◽  
Vol 46 ◽  
pp. 244-245
Author(s):  
Arthur J. Wasserman ◽  
David L. Christiansen ◽  
Y. Pedro Kato ◽  
Alvin J. Glasgold ◽  
Frederick H. Silver
Keyword(s):  
Electrolyte Solution ◽  
Reconstructive Surgery ◽  
Nasal Septum ◽  
Structural Changes ◽  
Septal Cartilage ◽  
Compositional Changes ◽  
Chemical Preservatives ◽  
Human Nasal Septum ◽  
Nasal Septal Cartilage ◽  
Extended Survival

Cartilage is used widely in reconstructive surgery. The inaccessiblity of live cartilage homografts and the limited extended survival record of preserved cartilage used as grafts (1) during surgery creates a need to understand the factors related to post-operative graft resorbtion. Structural changes in chemically preserved human nasal septum (HNS) and a decrease in the lower stiffness (2) of these tissues suggest compositional changes. The purpose of this study was to assess the chronic affects of the chemical preservatives MerthiolateR and AlcideR on HNS using S.E.M., T.E.M. and the proteoglycan specific electron dense stain quinolinic blue (QB) (3).HNS removed during rhinoplasty or after storage at 4 ° C for 6 months in Mertiolate R or AlcideR were diced into 1 mm cubes while immersed in fixative. Either 1.5% glutaraldehyde-4% paraformaldehyde in 0.1 M cacodylate with 4 mM CaCl2, or 2.5% glutaraldehyde in a critical electrolyte solution of 25mM Na acetate-0.3 M MgCl2 with 0.05% QB were used.

Effect of Gamma-Irradiation on Prenatal Development of the Nasal Septal Cartilage in CD-1 Mice

1994 ◽  
Vol 31 (3) ◽  
pp. 167-172 ◽  
Author(s):  
A. Yousef M. Saad
Keyword(s):  
Gamma Irradiation ◽  
Gamma Rays ◽  
Nasal Septum ◽  
Prenatal Development ◽  
Septal Cartilage ◽  
Whole Body ◽  
Maturation Process ◽  
Head Height ◽  
Mouse Fetuses ◽  
Nasal Septal Cartilage

The effects of gamma-rays on nasal septum development in CD-1 mouse fetuses subsequent to irradiation of their mothers were studied. Pregnant CD-1 mice exposed to 400 rads of whole body gamma-irradiation 12 days after gestation were sacrificed on day 18, post coltum. The fetuses were removed via laparotomy and analyzed. Data on head dimensions, including head height, width, and circumference were recorded. Fetal heads were then routinely prepared for histologic examination of the developing nasal septal cartilage. Analysis of data revealed significant reduction in litter size (p < .0025) and head measurements (p < .0005) of irradiated animals. Histologically, the nasal septa of Irradiated fetuses had retarded growth, were less differentiated, and smaller than those of control mice. Results suggest that gamma-irradiation has detrimental effects on litter and head sizes and may interfere with the cellular maturation process of nasal septal cartilage as well as other structures.

scholarly journals A RARE CASE OF CHONDROMA OF CARTILAGINOUS NASAL SEPTUM

2014 ◽  
Vol 04 (01) ◽  
pp. 120-122
Author(s):  
Shrinath D. Kamath P. ◽  
Kishore Shetty ◽  
Anusha Shetty ◽  
Michelle Mathias ◽  
Natashya Rent
Keyword(s):  
Nasal Septum ◽  
Rare Case ◽  
Benign Tumor ◽  
Histopathological Examination ◽  
Anterior Part ◽  
Septal Cartilage ◽  
Rare Occurrence ◽  
Left Nasal Cavity ◽  
Nasal Septal Cartilage ◽  
Anterior Septum

Abstract:Chondroma is a benign tumor of cartilaginous origin. Nasal septal chondromas are rare and almost always arise from the bony septum. Considering the very rare occurrence of chondroma from anterior part of the septum, we report a case of Chondroma of the nasal septal cartilage in an adult female, who presented with progressive unilateral nasal obstruction. CT scan showed the minimally enhancing lesion from the anterior septum confined to the left nasal cavity. Excision of the mass was done endoscopically. Histopathological examination of the specimen was suggestive of chondroma.

Tracheal stenosis: Repair with nasal septum: A case report

1982 ◽  
Vol 96 (4) ◽  
pp. 365-372 ◽  
Author(s):  
H. E. Porte
Keyword(s):  
Case Report ◽  
Tracheal Stenosis ◽  
Nasal Septum ◽  
Septal Cartilage ◽  
Post Traumatic ◽  
Nasal Septal Cartilage

SummaryPost-Traumatic upper tracheal stenosis in an 18-year old male treated by in-lay graft of nasal septal cartilage with attached mucosa.

scholarly journals Septal chondrocyte hypertrophy contributes to midface deformity in a mouse model of Apert syndrome

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bong-Soo Kim ◽  
Hye-Rim Shin ◽  
Hyun-Jung Kim ◽  
Heein Yoon ◽  
Young-Dan Cho ◽  
...  
Keyword(s):  
Nasal Septum ◽  
Septal Cartilage ◽  
Apert Syndrome ◽  
Careful Examination ◽  
Ethmoid Bone ◽  
Chondrocyte Hypertrophy ◽  
Respiratory Problems ◽  
Dimensional Changes ◽  
Midface Hypoplasia ◽  
Nasal Septal Cartilage

AbstractMidface hypoplasia is a major manifestation of Apert syndrome. However, the tissue component responsible for midface hypoplasia has not been elucidated. We studied mice with a chondrocyte-specific Fgfr2S252W mutation (Col2a1-cre; Fgfr2S252W/+) to investigate the effect of cartilaginous components in midface hypoplasia of Apert syndrome. In Col2a1-cre; Fgfr2S252W/+ mice, skull shape was normal at birth, but hypoplastic phenotypes became evident with age. General dimensional changes of mutant mice were comparable with those of mice with mutations in EIIa-cre; Fgfr2S252W/+, a classic model of Apert syndrome in mice. Col2a1-cre; Fgfr2S252W/+ mice showed some unique facial phenotypes, such as elevated nasion, abnormal fusion of the suture between the premaxilla and the vomer, and decreased perpendicular plate of the ethmoid bone volume, which are related to the development of the nasal septal cartilage. Morphological and histological examination revealed that the presence of increased septal chondrocyte hypertrophy and abnormal thickening of nasal septum is causally related to midface deformities in nasal septum-associated structures. Our results suggest that careful examination and surgical correction of the nasal septal cartilage may improve the prognosis in the surgical treatment of midface hypoplasia and respiratory problems in patients with Apert syndrome.

scholarly journals Temperature effect in physicochemical and bioactive behavior of biogenic hydroxyapatite obtained from porcine bones

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
P. A. Forero-Sossa ◽  
J. D. Salazar-Martínez ◽  
A. L. Giraldo-Betancur ◽  
B. Segura-Giraldo ◽  
E. Restrepo-Parra
Keyword(s):  
Structural Changes ◽  
Biological Fluid ◽  
Treatment Temperature ◽  
Mineral Phase ◽  
Calcination Temperatures ◽  
Bioactive Properties ◽  
Temperature Ranges ◽  
Compositional Changes ◽  
Type Substitution ◽  
Biogenic Hydroxyapatite

AbstractBiogenic hydroxyapatite (BHAp) is a widely used material in the biomedical area due to its similarities with the bone tissue mineral phase. Several works have been spotlighted on the thermal behavior of bone. However, little research has focused on determining the influence of calcination temperature in the physicochemical and bioactive properties of BHAp. In this work, a study of the physicochemical properties’ changes and bioactive response of BHAp produced from porcine femur bones using calcination temperatures between 900 to 1200 °C was conducted. The samples’ structural, morphological, and compositional changes were determined using XRD, SEM, and FTIR techniques. XRD results identified three temperature ranges, in which there are structural changes in BHAp samples and the presence of additional phases. Moreover, FTIR results corroborated that B-type substitution is promoted by increasing the heat treatment temperature. Likewise, samples were immersed in a simulated biological fluid (SBF), following the methodology described by Kokubo and using ISO 23317:2014 standard, for 3 and 7 days. FTIR and SEM results determined that the highest reaction velocity was reached for samples above 1000 °C, due to intensity increasing of phosphate and carbonate bands and bone-like apatite morphologies, compared to other temperatures evaluated.

Skull growth in achondroplasic (cn) mice; a craniometric study

Development ◽  
1975 ◽  
Vol 33 (4) ◽  
pp. 1013-1022
Author(s):  
Rosemary J. Jolly ◽  
W. J. Moore
Keyword(s):  
Cranial Base ◽  
Septal Cartilage ◽  
Skull Morphology ◽  
Condylar Cartilage ◽  
Central Section ◽  
Condylar Process ◽  
Skull Growth ◽  
Anterior Extension ◽  
Posterior Extension ◽  
Nasal Septal Cartilage

Skull morphology in achondroplasic (cn/cn) mice was compared with that of normal siblings in order to determine the effects of this chondrodystrophy on skull growth, particular attention being given to dimensions reflecting growth at the synchondroses of the cranial base, the nasal septal cartilage and the condylar cartilage of the mandible. The central section of the cranial base (basicranial axis) was reduced by 25 %, the length of the viscerocranium by 18 % and the length of the condylar process by 11 %. The evidence indicates that these reductions are due to diminished growth at respectively the spheno-occipital and midsphenoidal synchondroses, the nasal septal cartilage and the condylar cartilage. The relative sizes of the reductions in cranial base, viscerocranium and condylar process suggest that the growth of synchondrotic and septal cartilages is diminished to a greater extent than that of condylar cartilage. This finding is in agreement with the observations that condylar cartilage, unlike synchondrotic and septal cartilage, grows by surface apposition and that the principal defect in cn/cn mice is a disturbance of interstitial cartilaginous growth. The posterior extension of the basicranial axis of the cn/cn mice was reduced by 14 % and the anterior extension by 2 %. The width of the cranial base was decreased by 9 % and the angle between the basicranial axis and its anterior extension was decreased by 3 %. The length of theneurocranium was reduced by 19 % in the cn/cn animals while the volume of the endocranial cavity was diminished by only 18 %. The latter reduction is less than would be expected from the cube relationship between volume and linear dimensions but is readily accounted for by the lack of reduction in the height or width of the neurocranium, the slight flattening of the cranial base and the doming of the neurocranial vault.

The nasal cavity and paranasal sinuses

2013 ◽  
Author(s):  
Martin E. Atkinson
Keyword(s):  
Nasal Cavity ◽  
Nasal Septum ◽  
Goblet Cells ◽  
Septal Cartilage ◽  
Olfactory Mucosa ◽  
Nasal Cartilages ◽  
Orbital Roof ◽  
External Nose ◽  
The Right ◽  
Lateral Walls

The nasal cavity is the entrance to the respiratory tract. Its functions are to clean, warm, and humidify air as it is inhaled. Respiratory mucosa covered by pseudostratified ciliated epithelium and goblet cells, as described in Chapter 5 and illustrated in Figure 5.2B, lines the majority of the nasal cavity. The cilia and mucus trap particles, thus cleaning the air; the mucus also humidifies the air and warming is achieved through heat exchange from blood in the very vascular mucosa. The efficiency of all these processes is increased by expanding the surface of the nasal cavity by folds of bone. The nasal cavity also houses the olfactory mucosa for the special sense of olfaction although the olfactory mucosa occupies a very small proportion of the surface of the nasal cavity. The nasal cavity extends from the nostrils on the lower aspect of the external nose to the two posterior nasal apertures between the medial pterygoid plates where it is in continuation with the nasopharynx. Bear in mind that in dried or model skulls, the nasal cavity is smaller from front to back and the anterior nasal apertures seem extremely large because the cartilaginous skeleton of the external nose is lost during preparation of dried skulls. As you can see in Figure 27.1 , the nasal cavity extends vertically from the cribriform plate of the ethmoid at about the level of the orbital roof above to the palate, separating it from the oral cavity below. Figure 27.1 also shows that the nasal cavity is relatively narrow from side to side, especially in its upper part between the two orbits and widens where it sits between the right and left sides of the upper jaw below the orbits. The nasal cavity is completely divided into right and left compartments by the nasal septum . From the anterior view seen in Figure 27.1 , you can see that the surface area of lateral walls of the nasal cavity are extended by the three folds of bone, the nasal conchae. The skeleton of the external nose shown in Figure 27.2 comprises the nasal bones, the upper and lower nasal cartilages, the septal cartilage, and the cartilaginous part of the nasal septum.

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