scholarly journals Cobalt–deficiency–induced hyperhomocysteinaemia and oxidative status of cattle

2000 ◽  
Vol 83 (1) ◽  
pp. 3-6 ◽  
Author(s):  
G. I. Stangl ◽  
F. J. Schwarz ◽  
B. Jahn ◽  
M. Kirchgessner
Keyword(s):  
Plasma Proteins ◽  
Quantitative Difference ◽  
Thiobarbituric Acid ◽  
Oxidative Status ◽  
Cobalamin Deficiency ◽  
Vitamin B ◽  
Carbonyl Groups ◽  
Thiobarbituric Acid Reactive Substances ◽  
Cobalt Deficiency

In ruminants, Co is required for the synthesis of vitamin B12, which in turn is needed for the resynthesis of methionine by methylation of homocysteine and thus, cobalamin deficiency may induce hyperhomocysteinaemia which is brought into context with perturbations of the antioxidative–prooxidative balance. The present study was conducted to explore whether Co deficiency in cattle is also associated with homocysteine-induced disturbances of oxidative status. Co deficiency was induced in cattle by feeding two groups of animals on either a basal maize-silage-based diet that was moderately low in Co (83 μg Co/kg DM), or the same diet supplemented with Co to a total of 200 μg Co/kg DM, for 43 weeks. Co deficiency was apparent from a reduced vitamin B12 status in serum and liver and an accumulation of homocysteine in plasma which was in excess of 4·8 times higher in Co-deprived cattle than in controls. The much increased level of circulating homocysteine did not indicate severe disturbances in antioxidant–prooxidant balance as measured by individual markers of lipid peroxidation, protein oxidation, and the antioxidative defence system. There were no quantitative difference in plasma thiol groups, nor were there significant changes in concentrations of α-tocopherol, microsomal thiobarbituric acid-reactive substances and carbonyl groups in liver. However, there was a trend toward increased plasma carbonyl levels indicating a slight degradation of plasma proteins in the hyperhomocysteinaemic cattle. Analysis of the hepatic catalase (EC 1.11.1.6) activity revealed an 11 % reduction in Co-deficient cattle relative to the controls. These results indicate that long-term moderate Co deficiency may induce a severe accumulation of plasma homocysteine in cattle, but considerable abnormalities in oxidative status failed to appear.

scholarly journals Increased oxidative stress as a mechanism for decreased BDNF levels in acute manic episodes

2008 ◽  
Vol 30 (3) ◽  
pp. 243-245 ◽  
Author(s):  
Flávio Kapczinski ◽  
Benício N Frey ◽  
Ana C Andreazza ◽  
Márcia Kauer-Sant'Anna ◽  
Ângelo B M Cunha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bipolar Disorder ◽  
Neurotrophic Factor ◽  
Thiobarbituric Acid ◽  
Brain Derived Neurotrophic Factor ◽  
Oxidative Status ◽  
Control Group ◽  
Healthy Controls ◽  
Thiobarbituric Acid Reactive Substances ◽  
Low Levels

OBJECTIVE AND METHOD: There is a growing amount of data indicating that alterations in brain-derived neurotrophic factor and increased oxidative stress may play a role in the pathophysiology of bipolar disorder. In light of recent evidence demonstrating that brain-derived neurotrophic factor levels are decreased in situations of increased oxidative stress, we have examined the correlation between serum thiobarbituric acid reactive substances, a measure of lipid peroxidation, and serum brain-derived neurotrophic factor levels in bipolar disorder patients during acute mania and in healthy controls. RESULTS: Serum thiobarbituric acid reactive substances and brain-derived neurotrophic factor levels were negatively correlated in bipolar disorder patients (r = -0.56; p = 0.001), whereas no significant correlation was observed in the control group.. CONCLUSION: These results suggest that alterations in oxidative status may be mechanistically associated with abnormal low levels of brain-derived neurotrophic factor observed in individuals with bipolar disorder.

scholarly journals Effect of Storage Time on the Oxidative Status of Infant Formula

1970 ◽  
Vol 66 (2) ◽  
Author(s):  
Cristina Anamaria SEMENIUC ◽  
Camelia GUŞ ◽  
Ancuţa Mihaela ROTAR ◽  
Sonia Ancuţa SOCACI ◽  
Ramona SUHAROSCHI ◽  
...  
Keyword(s):  
Shelf Life ◽  
Infant Formula ◽  
Peroxide Value ◽  
Storage Time ◽  
Thiobarbituric Acid ◽  
Oxidative Status ◽  
Thiobarbituric Acid Reactive Substances

The aim of this study is to assess the oxidative status evolution of an infant formula product stored at 15°C during and after the shelf life. The initially phase of oxidation was monitories by the peroxide value (PV) and finally stage of oxidation by the thiobarbituric reactive substances (TBARS) test. The storage time has no significant effect on peroxide value (PV) and thiobarbituric acid reactive substances (TBARS).

scholarly journals PSXI-14 Supplementation with a blend containing Baccharis dracunculifolia and Tamarindus indica improve the oxidative state of steers in finishing phase

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 400-401
Author(s):  
Vicente A Diaz Avila ◽  
Edineia Bonin ◽  
Venício M Carvalho ◽  
Lucas Stafuza Moreira ◽  
Jurandir Fernando Comar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
Oxidative Status ◽  
Carbonyl Groups ◽  
Tamarindus Indica ◽  
Plasma Parameters ◽  
Protein Carbonyl Groups ◽  
Reducing Ability ◽  
Oxidative State

Abstract Tissue oxidative stress has been associated with low productivity of beef cattle. Supplementation of animals with natural antioxidants added to the diet may be an alternative to improve the tissue oxidative status and the productivity of steers at finishing phase. The present study evaluated whether the addition to the diet of a blend (Mix) containing Baccharis dracunculifolia (40%), Tamarindus indica seed (40%), cashew nutshell oil (10%) and clove oil (10%) modifies the oxidative state in the plasma and liver of steers (Angus x Zebu) in finishing phase. Forty animals were randomly distributed into four groups (ten animal per group), which received Mix respectively at the daily dose of 0.0, 2.0, 4.0 and 6.0 g per animal during 84 days. Blood and liver were taken at slaughter of the animals. Blood samples were also taken before starting the supplementation and served as control. The ferric reducing ability of plasma (FRAP), thiol groups and protein carbonyl groups were measured as plasma parameters of oxidative stress. The levels of thiobarbituric acid reactive substances (TBARS), protein carbonyl groups, reactive oxygen species (ROS), glutathione and the activity of catalase and superoxide dismutase (SOD) were measured as hepatic parameter of oxidative stress. Plasma levels of protein carbonyl groups were 30% lower in the animals supplemented with 4.0 and 6.0 g Mix and FRAP was 20% higher in the animals supplemented with 6.0 g Mix (P < 0.05; compared to the 0.0 g). Hepatic levels of TBARS were 38% lower in animals supplemented with 6.0 g Mix (P < 0.05). The other parameters were not modified. In conclusion, the supplementation of steers with Mix improved the systemic oxidative status and may be a complementary alternative to the diet of these animals in the termination phase.

scholarly journals Study of the Correlations among Some Parameters of the Oxidative Status, Gelatinases, and Their Inhibitors in a Group of Subjects with Metabolic Syndrome

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
E. Hopps ◽  
R. Lo Presti ◽  
M. Montana ◽  
B. Canino ◽  
M. R. Averna ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Oxidative Status ◽  
Entire Group ◽  
Carbonyl Groups ◽  
Total Antioxidant ◽  
Nitric Oxide Metabolites

Our aim was to examine some parameters of oxidative status, gelatinases, and their inhibitors and to evaluate their interrelationships in subjects with metabolic syndrome (MS). We enrolled 65 MS subjects, subdivided according to the presence or not of diabetes mellitus. We examined lipid peroxidation (expressed as thiobarbituric acid reacting substances, TBARS), protein oxidation (expressed as carbonyl groups), nitric oxide metabolites (NOx), total antioxidant status (TAS), MMP-2, MMP-9, TIMP-1, and TIMP-2. We found that MS subjects, diabetics and nondiabetics, showed an increase in TBARS, PC, and NOx. A significant decrease in TAS was observed only in nondiabetic MS subjects in comparison with diabetic MS subjects. We observed increased concentrations of MMP-2, MMP-9, TIMP-1, and TIMP-2, higher in diabetic subjects. Our data showed a positive correlation between TAS and MMP-2, TAS and MMP-9, and TAS and MMP-9/TIMP-1 and a negative correlation between TBARS and MMP-2 in diabetic MS subjects in the entire group. In MS subjects a prooxidant status and increased levels of gelatinases and their inhibitors are evident although the correlations between oxidative stress and MMPs or TIMPs are controversial and need further investigation.

scholarly journals THE COBALT ENGIMA - SOME OBSERVATIONS AND STRATEGIES FOR OTAGO AND SOUTHLAND

1989 ◽  
pp. 101-108 ◽  
Author(s):  
A.K. Metherell
Keyword(s):  
Vitamin B ◽  
Preventive Strategy ◽  
Research Station ◽  
Cobalt Deficiency ◽  
Vitamin B 12 ◽  
Lamb Growth ◽  
Term Maintenance ◽  
Pasture Plants

The role of cobalt (Co) and vitamin B 12 in animal nutrition is well understood, but the practical questions of predicting when and where a defnency is likely, and the most effective preventive strategy, remain unanswered in Southland and Otago. The incidence of Co deficiency in the region has recently increased, particularly where the problem had not previously been recognised In a long term maintenance Co topdressing experiment at Woodlands Research Station, pasture Co concentrations have been monitored since 1982 and lamb growth measured in two seasons. Cobalt deficiency has been observed only in the two driest seasons. It is postulated that soil manganese may influence the seasonal availability of Co to pasture plants. The effect of Co topdressing on pasture Co levels in the season of application was very short lived. Rate of cobalt sulphate application affected peak pasture concentration but had little effect on the duration of the pasture response. Lambs grazing treated plots did not gain a substantial reserve of vitamin 8, 2 Timing of Co application in relation to the likely period of deficiency is very Important. Keywords: pasture mineral content, lamb growth rate, vitamin B, 2, cobalt

scholarly journals Oxidation-antioxidant balance in the colon mucosa of rats at different times points after ceftriaxone administration

2017 ◽  
Vol 22 (1) ◽  
pp. 11-15
Author(s):  
Y. Holota ◽  
A. Bazan ◽  
G. Tolstanova
Keyword(s):  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Colon Mucosa ◽  
Thiol Groups ◽  
Thiobarbituric Acid Reactive Substances ◽  
Protein Thiol ◽  
Sod Activity ◽  
Control Value ◽  
Active Substances

Сeftriaxone administration for 14 days (300 mg/kg, i.m.) increased level of thiobarbituric acid reactive substances and decreased the activity of superoxide dismutase and catalase antioxidant enzymes in the colon mucosa of rats immediately after antibiotics injection. On the 29th day of the experiment (in 14 days after ceftriaxone withdrawal) the level of TBA-active substances still 2.5-fold (P <0.05) exceeded the control value and SOD activity remained below control values to the 72nd day of the experiment. These were accompanied by decreased level of protein thiol groups in 15 and 29 days 1.9-fold (P <0.05) and 1.4-fold (P = 0.08), respectively. Thus antibiotics can lead to long-term oxidative disturbance in the colon mucosa of rats.

Effects of hypoxia and dietary vitamin E on growth performance and oxidative status of Cirrhinus mrigala (Ham., 1822)

2017 ◽  
Vol 67 (2) ◽  
pp. 133-148 ◽  
Author(s):  
Tincy Varghese ◽  
Asim Kumar Pal ◽  
Narottam Prasad Sahu ◽  
Puthiyottil Mishal ◽  
Subrata Dasgupta
Keyword(s):  
Fatty Acids ◽  
Superoxide Dismutase ◽  
Fatty Acid ◽  
Vitamin E ◽  
Polyunsaturated Fatty Acids ◽  
Peroxide Value ◽  
Thiobarbituric Acid ◽  
Oxidative Status ◽  
Dietary Vitamin ◽  
Thiobarbituric Acid Reactive Substances

Vitamin E is of importance for several physiological processes, some of which also apply to fish. Here, we conducted an experiment to assess the effect of environmental hypoxia and dietary vitamin E on oxidative status and tissue injury in a bottom dwelling carp,Cirrhinus mrigala(Ham., 1822). The four treatments combined oxygen availability (Normoxia/Hypoxua) and Vitamin E presence/absence. Lipid peroxidation parameters such as thiobarbituric acid reactive substances (TBAR), peroxide value (PV), polyunsaturated fatty acids/saturated fatty acid (PUFA/SFA) ratio, catalase (CAT), superoxide dismutase (SOD) and erythrocyte fragility were assessed. The results indicate that exposure to hypoxia elevates these parameters. However, the supplementation of vitamin E via the diet effectively reduced erythrocyte membrane damage (EF) and myeloperoxidase activity (MPO), which were enhanced by the exposure to hypoxia. Dietary vitamin E also improved antioxidant enzyme status in the hypoxia exposed fish, as indicated by the decreased catalase and superoxide dismutase activities. Vitamin E supplementation also compensated for increased levels of peroxide value, thiobarbituric acid reactive substances, and an increased polyunsaturated fatty acids/saturated fatty acid ratio resulting from the exposure to hypoxia. Overall, it can be concluded that fortification of vitamin E in the diet of this carp species, and possibly other fish that live under hypoxic conditions, can restore the antioxidant status and well-being to some extent.

scholarly journals Influence of Sulfur Induced Stress on Oxidative Status and Antioxidative Machinery in Leaves of Allium cepa L.

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Neelam Chandra ◽  
Nalini Pandey
Keyword(s):  
Thiobarbituric Acid ◽  
Oxidative Status ◽  
Thiobarbituric Acid Reactive Substances ◽  
Culture Experiment ◽  
Induced Stress ◽  
Allium Cepa L ◽  
Catalase Peroxidase ◽  
Sulfur Supply ◽  
Metabolic Activities ◽  
Different Levels

A pot culture experiment was carried out to assess the effect of sulfur stress on growth, oxidative status, and antioxidative metabolism. Onion plants were treated with three different levels of sulfur, namely, 1.0, 4.0, and 8.0 mM S L−1. Plants raised with 4.0 mM S L−1 represent sufficient growth for the best vegetative yield. Plants supplied with 1.0 and 8.0 mM S L−1 showed retarded growth, chlorosis, and reduction in biomass and photoassimilatory pigments. Tissue sulfur concentration and cysteine were increased with increasing sulfur supply. Carbohydrates (sugars and starch) were accumulated in sulfur stressed plants. Hydrogen peroxide levels were increased in sulfur stressed plants. Thiobarbituric acid reactive substances levels were also increased which was an indicator of lipid peroxidation. Enzymatic (superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, and glutathione reductase) and nonenzymatic (asorbate) antioxidative components were enhanced in sulfur stressed plants. Glutathione was increased with increasing sulfur supply. The present study showed that the adverse effects of inadequate sulfur supply result in irregular metabolic activities and antioxidant machinery.

scholarly journals Some biochemical parameters of the synovial liquid for estimation of effectiveness of the treatment of the knee joint osteoarthrosis

2012 ◽  
Vol 58 (2) ◽  
pp. 230-236 ◽  
Author(s):  
M.G. Meshcheryakova ◽  
Ya.G. Trilis ◽  
N.V. Kirillova ◽  
A.T. Alpatova ◽  
I.A. Muhin ◽  
...  
Keyword(s):  
Knee Joint ◽  
Biochemical Parameters ◽  
Specific Treatment ◽  
Thiobarbituric Acid ◽  
Oxidative Modification ◽  
Total Protein Content ◽  
Laboratory Diagnostics ◽  
Carbonyl Groups ◽  
Thiobarbituric Acid Reactive Substances ◽  
Oxidative Modification Of Proteins

The knee joint osteoarthrosis is accompanied by activation of the oxidative stress in the synovial liquid. Specific treatment decreased or even normalized such biochemical parameters of the synovial liquid as the carbonyl groups, thiobarbituric acid reactive substances (TBARS) and total protein content. The most demonstrative changes were found for early and late markers of the oxidative modification of proteins. These parameters may be used in laboratory diagnostics of the depth of the degenerative-dystrophic process in the knee joint and for the estimation of the effectiveness of the treatment.

Sign in / Sign up

Export Citation Format

Share Document