scholarly journals A New Sample Preparation System for Micro-14C Dating of Glacier Ice with a First Application to a High Alpine Ice Core from Colle Gnifetti (Switzerland)

Radiocarbon ◽  
2017 ◽  
Vol 60 (2) ◽  
pp. 517-533 ◽  
Author(s):  
Helene Hoffmann ◽  
Susanne Preunkert ◽  
Michel Legrand ◽  
David Leinfelder ◽  
Pascal Bohleber ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Low Cost ◽  
Ice Core ◽  
Mass Range ◽  
Radiometric Dating ◽  
14C Dating ◽  
Alpine Glacier ◽  
Glacier Ice ◽  
Depth Relationship ◽  
Sample Age

AbstractRadiometric dating of glacier ice is an essential tool where stratigraphic dating methods cannot be applied. This study focuses on Alpine glacier ice and presents a new sample preparation system for dating of glacier ice samples via radiocarbon (14C) dating of the microscopic particulate organic carbon (POC) fraction incorporated in the ice matrix. An adaptable, low-cost inline filtration-oxidation-unit (REFILOX) has been developed, which for the first time unifies all sample preparation steps from ice filtration to CO2quantification in one closed setup. A systematic14C investigation of modern European aerosol samples revealed that a POC combustion temperature of 340°C provides the best representation of the real sample age. A very low process blank of maximally 0.3±0.1 µgC now enables14C dating of high Alpine ice samples, where POC concentrations are generally low (typically 10–50 µgC/kg), in an ice sample mass range of 300–500 g. In a first successful application, the method was used to obtain age constraints for an ice core from the cold, high Alpine firn saddle Colle Gnifetti (Switzerland). Analysis of the bottom ice core sections revealed a basal age of 4171–3923 cal yr BP but also a so far enigmatic discontinuity in the age-depth relationship.

scholarly journals Radiocarbon dating of glacier ice

2016 ◽  
Author(s):  
Chiara Uglietti ◽  
Alexander Zapf ◽  
Theo M. Jenk ◽  
Sönke Szidat ◽  
Gary Salazar ◽  
...  
Keyword(s):  
Information Needs ◽  
Ice Core ◽  
Ice Cores ◽  
European Alps ◽  
Carbonaceous Aerosols ◽  
14C Dating ◽  
Tropical Regions ◽  
Annual Layer ◽  
Glacier Ice ◽  
Depth Relationship

Abstract. High altitude glaciers and ice caps from mid-latitudes and tropical regions contain valuable signals of past climatic and environmental conditions as well as human activities, but for a meaningful interpretation this information needs to be placed in a precise chronological context. For dating the upper part of ice cores from such sites several relatively precise methods exist, but they fail in the older and deeper part, where plastic deformation of the ice results in strong annual layer thinning and a non-linear age-depth relationship. If sufficient organic matter such as plant, wood or insect fragments were found, radiocarbon (14C) analysis had thus been the only option for a direct and absolute dating of deeper ice core sections. However such fragments are rarely found and even then very likely not at the depths and in the resolution desired. About 10 years ago, a new, complementary dating tool was therefore introduced by our group. It is based on extracting the μg-amounts of the water-insoluble organic carbon (WIOC) fraction of carbonaceous aerosols embedded in the ice matrix for subsequent 14C dating. Meanwhile this new approach was improved considerably, thereby reducing the measurement time and improving the overall precision. Samples with ~ 10 μg WIOC mass can now be dated with reasonable uncertainty of around 10–20 % (variable depending on sample age). This requires about 100 to 500 g of ice considering the WIOC concentrations typically found in mid- and low-latitude glacier ice. Dating polar ice with satisfactory age precision is still not possible since WIOC concentrations are around one order of magnitude lower. The accuracy of the 14C WIOC method was validated by applying it to independently dated ice. With this method the deepest parts of the ice cores from Colle Gnifetti and Mt. Ortles glacier in the European Alps, Illimani glacier in the Bolivian Andes, Tsambagarav ice cap in the Mongolian Altai, and Belukha glacier in the Siberian Altai have been dated. In all cases a strong annual layer thinning towards bedrock was observed and the oldest ages obtained were in the range of 10 000 yrs. 14C WIOC-dating was not only crucial for interpretation of the embedded environmental and climatic histories, but additionally gave a better insight into glacier flow dynamics close to bedrock and past glacier coverage. For this the availability of multiple dating points in the deepest parts was essential, which is the strength of the presented WIOC 14C-dating method, allowing determination of absolute ages from principally every piece of ice.

scholarly journals Radiocarbon dating of glacier ice: overview, optimisation, validation and potential

2016 ◽  
Vol 10 (6) ◽  
pp. 3091-3105 ◽  
Author(s):  
Chiara Uglietti ◽  
Alexander Zapf ◽  
Theo Manuel Jenk ◽  
Michael Sigl ◽  
Sönke Szidat ◽  
...  
Keyword(s):  
Information Needs ◽  
Ice Core ◽  
Ice Cores ◽  
European Alps ◽  
Carbonaceous Aerosols ◽  
14C Dating ◽  
Tropical Regions ◽  
Annual Layer ◽  
Glacier Ice ◽  
Depth Relationship

Abstract. High-altitude glaciers and ice caps from midlatitudes and tropical regions contain valuable signals of past climatic and environmental conditions as well as human activities, but for a meaningful interpretation this information needs to be placed in a precise chronological context. For dating the upper part of ice cores from such sites, several relatively precise methods exist, but they fail in the older and deeper parts, where plastic deformation of the ice results in strong annual layer thinning and a non-linear age–depth relationship. If sufficient organic matter such as plant, wood or insect fragments were found, radiocarbon (14C) analysis would have thus been the only option for a direct and absolute dating of deeper ice core sections. However such fragments are rarely found and, even then, they would not be very likely to occur at the desired depth and resolution. About 10 years ago, a new, complementary dating tool was therefore introduced by our group. It is based on extracting the µg-amounts of the water-insoluble organic carbon (WIOC) fraction of carbonaceous aerosols embedded in the ice matrix for subsequent 14C dating. Since then this new approach has been improved considerably by reducing the measurement time and improving the overall precision. Samples with ∼ 10 µg WIOC mass can now be dated with reasonable uncertainty of around 10–20 % (variable depending on sample age). This requires about 300 to 800 g of ice for WIOC concentrations typically found in midlatitude and low-latitude glacier ice. Dating polar ice with satisfactory age precision is still not possible since WIOC concentrations are around 1 order of magnitude lower. The accuracy of the WIOC 14C method was validated by applying it to independently dated ice. With this method, the deepest parts of the ice cores from Colle Gnifetti and the Mt Ortles glacier in the European Alps, Illimani glacier in the Bolivian Andes, Tsambagarav ice cap in the Mongolian Altai, and Belukha glacier in the Siberian Altai have been dated. In all cases a strong annual layer thinning towards the bedrock was observed and the oldest ages obtained were in the range of 10 000 years. WIOC 14C dating was not only crucial for interpretation of the embedded environmental and climatic histories, but additionally gave a better insight into glacier flow dynamics close to the bedrock and past glacier coverage. For this the availability of multiple dating points in the deepest parts was essential, which is the strength of the presented WIOC 14C dating method, allowing determination of absolute ages from principally every piece of ice.

New δ18Oatm, δ18Oice and δDice profiles from deep ice of the TALDICE core

2020 ◽  
Author(s):  
Ilaria Crotti ◽  
Carlo Barbante ◽  
Massimo Frezzotti ◽  
Wei Jiang ◽  
Amaelle Landais ◽  
...  
Keyword(s):  
High Resolution ◽  
Ice Core ◽  
Ice Sheet ◽  
Ice Cores ◽  
Radiometric Dating ◽  
The Core ◽  
Climatic Signal ◽  
High Frequency Variability ◽  
The One ◽  
Depth Relationship

<p>The study of the deep portions of ice cores still represents a poorly explored field due to the presence of processes acting in the lowermost layers and possibly affecting the preservation of the original climatic signal. For the 1620 m TALDICE ice core, drilled at Talos Dome (East Antarctica), the high-resolution climate reconstruction and chronology definition are available only until the depth of ~1450 m (150 kyr BP) (Stenni et al., 2011, Bazin et al., 2013). Our aim is to investigate the portion below 1460 m depth to the bottom of the core, where radargrams show the presence of an unconformity in the ice sheet, to define a preliminary chronology and identify a discernible climatic signal.</p><p>Here we present the new TALDICE δ<sup>18</sup>O<sub>atm</sub> record in the air bubbles, in association with the new high-resolution δ<sup>18</sup>O<sub>ice</sub> and δD<sub>ice</sub> profiles and an <sup>81</sup>Kr radiometric date. New 46 measurements of δ<sup>18</sup>O<sub>atm  </sub>allowed to increase the resolution of the available profile from 1357 to 1553.95 m depth and to extend the record till the bottom of the core at 1617 m depth. The comparison between the δ<sup>18</sup>O<sub>atm</sub> profile of TALDICE and the one of EPICA Dome C (EDC) ice core (Extier et al., 2018) allows to solidly define a preliminary age-depth relationship for the TALDICE core until 1500 m depth, where the gas age is estimated to be ~200 kyr BP. Below 1500 m, supplementary δ<sup>18</sup>O<sub>atm </sub>measurements will be needed to identify older precession cycles and to extend the age-depth relationship further back in time. On the other hand, the high-resolution isotopic profiles in the ice (<sup>18</sup>O/<sup>16</sup>O and D/H ratios) obtained below the depth of 1528 m and compared with the EDC ones suggest that the climatic signal in the ice is preserved until to the lower level of 1547.8 m, which is dated back to 343 kyr BP. However, the lack of similarities with the EDC water isotopes record below this depth, in spite of the <sup>81 </sup>Kr radiometric age 459 ± 50 kyr BP at the depth of 1574-1578 m, indicates the missing of the MIS 11 in the isotopic profiles. Moreover, the increase of high-frequency variability in the δ<sup>18</sup>O<sub>ice</sub> and δD<sub>ice</sub> below 1547.8 m depth implies that this part of the core lays in an area of the ice sheet characterized by different properties in comparison to the ice above.</p><p>Additional δ<sup>18</sup>O<sub>atm</sub>, <sup>40</sup>Ar, δ<sup>18</sup>O<sub>ice,</sub> and δD<sub>ice</sub> measurements will be performed in the lowermost portion of the core and the results will be compared with the new <sup>81</sup>Kr radiometric dating at the depth of 1560-1564 m and 1614-1619 m to better constrain the chronology and to investigate the ice properties in the deeper portion of the core.</p>

To Eliminate Curtain Effect of FIB TEM Samples by a Combination of Sample Dicing and Backside Milling

2014 ◽  
Author(s):  
Jian-Shing Luo ◽  
Hsiu Ting Lee
Keyword(s):  
Sample Preparation ◽  
Focused Ion Beam ◽  
Preparation Method ◽  
Low Cost ◽  
Ion Beam ◽  
Sample Preparation Method ◽  
Site Specific ◽  
Dual Beam ◽  
Transmission Electron

Abstract Several methods are used to invert samples 180 deg in a dual beam focused ion beam (FIB) system for backside milling by a specific in-situ lift out system or stages. However, most of those methods occupied too much time on FIB systems or requires a specific in-situ lift out system. This paper provides a novel transmission electron microscopy (TEM) sample preparation method to eliminate the curtain effect completely by a combination of backside milling and sample dicing with low cost and less FIB time. The procedures of the TEM pre-thinned sample preparation method using a combination of sample dicing and backside milling are described step by step. From the analysis results, the method has applied successfully to eliminate the curtain effect of dual beam FIB TEM samples for both random and site specific addresses.

scholarly journals Investigation of a cold-based ice apron on a high-mountain permafrost rock wall using ice texture analysis and micro-14C dating: a case study of the Triangle du Tacul ice apron (Mont Blanc massif, France)

2021 ◽  
pp. 1-8
Author(s):  
Grégoire Guillet ◽  
Susanne Preunkert ◽  
Ludovic Ravanel ◽  
Maurine Montagnat ◽  
Ronny Friedrich
Keyword(s):  
Radiocarbon Dating ◽  
Boundary Migration ◽  
Ice Core ◽  
Ice Cores ◽  
Shear Plane ◽  
High Mountain ◽  
14C Dating ◽  
Rock Wall ◽  
Major Mechanism ◽  
Mont Blanc Massif

Abstract The current paper studies the dynamics and age of the Triangle du Tacul (TDT) ice apron, a massive ice volume lying on a steep high-mountain rock wall in the French side of the Mont-Blanc massif at an altitude close to 3640 m a.s.l. Three 60 cm long ice cores were drilled to bedrock (i.e. the rock wall) in 2018 and 2019 at the TDT ice apron. Texture (microstructure and lattice-preferred orientation, LPO) analyses were performed on one core. The two remaining cores were used for radiocarbon dating of the particulate organic carbon fraction (three samples in total). Microstructure and LPO do not substantially vary with along the axis of the ice core. Throughout the core, irregularly shaped grains, associated with strain-induced grain boundary migration and strong single maximum LPO, were observed. Measurements indicate that at the TDT ice deforms under a low strain-rate simple shear regime, with a shear plane parallel to the surface slope of the ice apron. Dynamic recrystallization stands out as the major mechanism for grain growth. Micro-radiocarbon dating indicates that the TDT ice becomes older with depth perpendicular to the ice surface. We observed ice ages older than 600 year BP and at the base of the lowest 30 cm older than 3000 years.

scholarly journals Glacier ice archives nearly 15,000-year-old microbes and phages

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Zhi-Ping Zhong ◽  
Funing Tian ◽  
Simon Roux ◽  
M. Consuelo Gazitúa ◽  
Natalie E. Solonenko ◽  
...  
Keyword(s):  
Climate Change ◽  
Ice Core ◽  
Ice Cores ◽  
Single Species ◽  
Amplicon Sequencing ◽  
Future Climate Change ◽  
Climate Conditions ◽  
Glacier Ice ◽  
Sampling Procedures ◽  
Functional Genome

Abstract Background Glacier ice archives information, including microbiology, that helps reveal paleoclimate histories and predict future climate change. Though glacier-ice microbes are studied using culture or amplicon approaches, more challenging metagenomic approaches, which provide access to functional, genome-resolved information and viruses, are under-utilized, partly due to low biomass and potential contamination. Results We expand existing clean sampling procedures using controlled artificial ice-core experiments and adapted previously established low-biomass metagenomic approaches to study glacier-ice viruses. Controlled sampling experiments drastically reduced mock contaminants including bacteria, viruses, and free DNA to background levels. Amplicon sequencing from eight depths of two Tibetan Plateau ice cores revealed common glacier-ice lineages including Janthinobacterium, Polaromonas, Herminiimonas, Flavobacterium, Sphingomonas, and Methylobacterium as the dominant genera, while microbial communities were significantly different between two ice cores, associating with different climate conditions during deposition. Separately, ~355- and ~14,400-year-old ice were subject to viral enrichment and low-input quantitative sequencing, yielding genomic sequences for 33 vOTUs. These were virtually all unique to this study, representing 28 novel genera and not a single species shared with 225 environmentally diverse viromes. Further, 42.4% of the vOTUs were identifiable temperate, which is significantly higher than that in gut, soil, and marine viromes, and indicates that temperate phages are possibly favored in glacier-ice environments before being frozen. In silico host predictions linked 18 vOTUs to co-occurring abundant bacteria (Methylobacterium, Sphingomonas, and Janthinobacterium), indicating that these phages infected ice-abundant bacterial groups before being archived. Functional genome annotation revealed four virus-encoded auxiliary metabolic genes, particularly two motility genes suggest viruses potentially facilitate nutrient acquisition for their hosts. Finally, given their possible importance to methane cycling in ice, we focused on Methylobacterium viruses by contextualizing our ice-observed viruses against 123 viromes and prophages extracted from 131 Methylobacterium genomes, revealing that the archived viruses might originate from soil or plants. Conclusions Together, these efforts further microbial and viral sampling procedures for glacier ice and provide a first window into viral communities and functions in ancient glacier environments. Such methods and datasets can potentially enable researchers to contextualize new discoveries and begin to incorporate glacier-ice microbes and their viruses relative to past and present climate change in geographically diverse regions globally.

scholarly journals South Pole glacial climate reconstruction from multi-borehole laser particulate stratigraphy

2013 ◽  
Vol 59 (218) ◽  
pp. 1117-1128 ◽  
Author(s):  
Keyword(s):  
Particle Physics ◽  
Volcanic Ash ◽  
Ice Core ◽  
Hot Water ◽  
Radiometric Dating ◽  
South Pole ◽  
Last Glacial Period ◽  
Annual Layer ◽  
Paleoclimate Records ◽  
The Last Glacial

AbstractThe IceCube Neutrino Observatory and its prototype, AMANDA, were built in South Pole ice, using powerful hot-water drills to cleanly bore >100 holes to depths up to 2500 m. The construction of these particle physics detectors provided a unique opportunity to examine the deep ice sheet using a variety of novel techniques. We made high-resolution particulate profiles with a laser dust logger in eight of the boreholes during detector commissioning between 2004 and 2010. The South Pole laser logs are among the most clearly resolved measurements of Antarctic dust strata during the last glacial period and can be used to reconstruct paleoclimate records in exceptional detail. Here we use manual and algorithmic matching to synthesize our South Pole measurements with ice-core and logging data from Dome C, East Antarctica. We derive impurity concentration, precision chronology, annual-layer thickness, local spatial variability, and identify several widespread volcanic ash depositions useful for dating. We also examine the interval around ∼74 ka recently isolated with radiometric dating to bracket the Toba (Sumatra) supereruption.

Capturing Glacier-Wide Cryoseismicity With Distributed Acoustic Sensing

2021 ◽  
Author(s):  
Fabian Walter ◽  
Patrick Paitz ◽  
Andreas Fichtner ◽  
Pascal Edme ◽  
Wojciech Gajek ◽  
...  
Keyword(s):  
Fiber Optic ◽  
Flow Line ◽  
Ground Deformation ◽  
Sensor Coverage ◽  
Mountain Glacier ◽  
Acoustic Sensing ◽  
Alpine Glacier ◽  
Glacier Ice ◽  
Distributed Acoustic Sensing ◽  
Ice Sheet Dynamics

<p>Over the past 1-2 decades, seismological measurements have provided new and unique insights into glacier and ice sheet dynamics. At the same time, sensor coverage is typically limited in harsh glacial environments with littile or no access. Turning kilometer-long fiber optic cables placed on the Earth’s surface into thousands of seismic sensors, Distributed Acoustic Sensing (DAS) may overcome the limitation of sensor coverage in the cryosphere.</p><p>First DAS applications on the Greenland and Antarctic ice sheets and on Alpine glacier ice have highlighted the technique’s superiority. Signals of natural and man-made seismic sources can be resolved with an unrivaled level of detail. This offers glaciologists new perspectives to interpret their seismograms in terms of ice structure, basal boundary conditions and source locations. However, previous studies employed only relatively small network scales with a point-like borehole deployment or < 1 km cable aperture at the ice surface.</p><p>Here we present a DAS installation, which aims to cover the majority of an Alpine glacier catchment: For one month in summer 2020 we deployed a 9 km long fiber optic cable on Rhonegletscher, Switzerland, and gathered continuous DAS data. The cable followed the glacier’s central flow line starting in the lowest kilometer of the ablation zone and extending well into the accumulation area. Even for a relatively small mountain glacier such as Rhonegletscher, cable deployment was a considerable logistical challenge. However, initial data analysis illustrates the benefit compared to conventional cryoseismological instrumentation: DAS measurements capture ground deformation over many octaves, including typical high-frequency englacial sources (10s to 100s of Hz) related to crevasse formation and basal sliding as well as long period signals (10s to 100s of seconds) of ice deformation. Depending on the presence of a snow cover, DAS records contain strong environmental noise (wind, meltwater flow, precipitation) and thus exhibit lower signal-to-noise ratios compared to conventional on-ice seismic installations. This is nevertheless outweighed by the advantage of monitoring ground unrest and ice deformation of nearly an entire glacier. We present a first compilation of signal and noise records and discuss future directions to leverage DAS data sets in glaciological research.</p><p> </p><p> </p><p> </p>

A comparative study of qualitative immunochemical screening assays for the combined measurement of T-2/HT-2 in cereals and cereal-based products

2011 ◽  
Vol 4 (4) ◽  
pp. 385-394 ◽  
Author(s):  
J. Meneely ◽  
F. Ricci ◽  
S. Vesco ◽  
M. Abouzied ◽  
M. Sulyok ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Low Cost ◽  
Ease Of Use ◽  
The Other ◽  
Accuracy And Precision ◽  
Cost Implications ◽  
Electrochemical Array ◽  
The Cost ◽  
Preparation Techniques ◽  
Speed Of Analysis

Many different immunochemical platforms exist for the screening of naturally occurring contaminants in food from the low cost enzyme linked immunosorbent assays (ELISA) to the expensive instruments such as optical biosensors based on the phenomenon of surface plasmon resonance (SPR). The primary aim of this study was to evaluate and compare a number of these platforms to assess their accuracy and precision when applied to naturally contaminated samples containing HT-2/T-2 mycotoxins. Other important factors considered were the speed of analysis, ease of use (sample preparation techniques and use of the equipment) and ultimately the cost implications. The three screening procedures compared included an SPR biosensor assay, a commercially available ELISA and an enzymelinked immunomagnetic electrochemical array (ELIME array). The qualitative data for all methods demonstrated very good overall agreements with each other, however on comparison with mass spectrometry confirmatory results, the ELISA and SPR assay performed slightly better than the ELIME array, exhibiting an overall agreement of 95.8% compared to 91.7%. Currently, SPR is more costly than the other two platforms and can only be used in the laboratory whereas in theory both the ELISA and ELIME array are portable and can be used in the field, but ultimately this is dependent on the sample preparation techniques employed. Sample preparative techniques varied for all methods evaluated, the ELISA was the most simple to perform followed by that of the SPR method. The ELIME array involved an additional clean-up step thereby increasing both the time and cost of analysis. Therefore in the current format, field use would not be an option for the ELIME array. In relation to speed of analysis, the ELISA outperformed the other methods.

scholarly journals Melt regimes, stratigraphy, flow dynamics and glaciochemistry of three glaciers in the Alaska Range

2012 ◽  
Vol 58 (207) ◽  
pp. 99-109 ◽  
Author(s):  
Seth Campbell ◽  
Karl Kreutz ◽  
Erich Osterberg ◽  
Steven Arcone ◽  
Cameron Wake ◽  
...  
Keyword(s):  
Ground Penetrating Radar ◽  
Ice Core ◽  
Chemical Diffusion ◽  
Flow Dynamics ◽  
Alaska Range ◽  
Ice Volume ◽  
Glacier Ice ◽  
Age Model ◽  
Ground Penetrating ◽  
Climate Studies

AbstractWe used ground-penetrating radar (GPR), GPS and glaciochemistry to evaluate melt regimes and ice depths, important variables for mass-balance and ice-volume studies, of Upper Yentna Glacier, Upper Kahiltna Glacier and the Mount Hunter ice divide, Alaska. We show the wet, percolation and dry snow zones located below ~2700ma.s.l., at ~2700 to 3900ma.s.l. and above 3900ma.s.l., respectively. We successfully imaged glacier ice depths upwards of 480 m using 40-100 MHz GPR frequencies. This depth is nearly double previous depth measurements reached using mid-frequency GPR systems on temperate glaciers. Few Holocene-length climate records are available in Alaska, hence we also assess stratigraphy and flow dynamics at each study site as a potential ice-core location. Ice layers in shallow firn cores and attenuated glaciochemical signals or lacking strata in GPR profiles collected on Upper Yentna Glacier suggest that regions below 2800ma.s.l. are inappropriate for paleoclimate studies because of chemical diffusion, through melt. Flow complexities on Kahiltna Glacier preclude ice-core climate studies. Minimal signs of melt or deformation, and depth-age model estimates suggesting ~4815 years of ice on the Mount Hunter ice divide (3912ma.s.l.) make it a suitable Holocene-age ice-core location.

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