Influence of soybean bioactive peptides on growth performance, nutrient utilisation, digestive tract development and intestinal histology in broilers

Author(s):  
M. R. Abdollahi ◽  
F. Zaefarian ◽  
Y. Gu ◽  
W. Xiao ◽  
J. Jia ◽  
...  

SummaryA biologically active peptide derived from soybeans by enzymatic hydrolysis was evaluated for its potential benefits on chicken growth performance, apparent ileal nutrient digestibility and intestinal histology in young broilers. Seven broiler starter diets, based on maize and soybean meal, were formulated to contain 0.0, 1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 g/kg of a commercial soybean bioactive peptide (SBP) product (Fortide, Chengdu Mytech Biotech Co. Ltd., Chengdu, Sichuan, China). All diets were equivalent in respect of energy density, and digestible protein, amino acids, and other nutrients. A total of 336, one-day-old male broilers (Ross 308) were allocated to 42 cages (eight birds/cage), which were randomly assigned to the six dietary treatments. There was no significant effect of SBP on weight gain and feed intake of the birds. A significant (P < 0.01) effect of SBP was observed for FCR. Inclusion of 1.0, 2.0, 3.0 and 4.0 g SBP/kg of feed resulted in similar FCR values to the diet with no SBP, addition of SBP to the diets at 5.0 and 6.0 g/kg of feed resulted in lower (P < 0.05) FCR compared to the diet with no SBP. Inclusion of SBP had no effect (P > 0.05) on apparent ileal digestibility of nutrients and energy utilisation. Though not statistically significant, SBP inclusion, regardless of level, resulted in 5.7% and 6.3% increases in digestibility of dry matter and nitrogen, respectively. Birds receiving no SBP had the shortest villi and those fed SBP at 3.0 and 6.0 g/kg of feed tended (P = 0.075) to have the greatest villus height. The current findings suggested that including SBP in broiler diets may benefit production through improving feed efficiency, and, to some extent, nutrient digestion and intestinal histology parameters.

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 101-102
Author(s):  
Jaron R Lewton ◽  
Adrienne Woodward ◽  
Ronny Moser ◽  
Kyan M Thelen ◽  
Adam J Moeser ◽  
...  

Abstract A study was conducted to evaluate the effects of a multi-strain Bacillus subtilis-based direct-fed microbial (DFM) on apparent digestibility and colonic pH of nursery pigs. Eighty pigs, of equal number of barrows and gilts (initial BW: 6.99 ± 1.67 kg), were weaned at 21 ± 1 d and randomly allotted to sixteen pens, with five pigs per pen. Two dietary treatments were implemented, a basal control (CON) and a control plus DFM (DFM). Both diets were corn, soybean meal, and distillers dried grains based, formulated to meet all or exceed all nutritional requirements, and manufactured on site. Diets were fed for 42 days. Performance measures were recorded weekly. On d 21 and 42 of the experiment, one pig per pen was randomly selected and euthanized, with equal number of males and females represented. Digestibility of specific nutrients was evaluated within the duodenum, jejunum, ileum, ascending and distal colon. There were no overall differences in growth performance. Overall means ± SD were 0.51 ± 0.05 kg/d, 0.79 ± 0.05 kg/d and 0.66 ± 0.05 for ADG, ADFI, and G:F, respectively. Digestibility of tryptophan within the jejunum tended (P = 0.06) to increase with addition of DFM, as did cysteine (P = 0.12) and methionine (P = 0.10). The analysis also suggested that the impact of the DFM on the digestibility of amino acids may be early in the nursery phase. The pH of contents in ascending colon, a possible indicator of varied fiber digestion, did not differ. Likewise, no differences were observed between treatment in apparent total tract nitrogen and energy digestibility (analysis of distal colon contents). The addition of a multi-strain Bacillus subtilis-based DFM appears to impact digestibility of select amino acids depending upon location in the gastrointestinal tract.


Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 375
Author(s):  
Sheena Kim ◽  
Jin Ho Cho ◽  
Younghoon Kim ◽  
Hyeun Bum Kim ◽  
Minho Song

The present study was conducted to evaluate the effects of replacing corn with brown rice on growth performance, nutrient digestibility, carcass characteristics, and gut microbiota of growing and finishing pigs. A total of 100 growing pigs (23.80 ± 2.96 kg BW; 10 weeks of age) were randomly allotted to 4 dietary treatments (5 pigs/pen; 5 replicates/treatment) in a randomized complete block design (block = BW) as follows: corn-soybean meal basal diet (CON) and replacing corn with 50% (GBR50), 75% (GBR75), and 100% (GBR100) of ground brown rice. Each trial phase was for 6 weeks. During the growing period, there were no differences on growth performance and nutrient digestibility among dietary treatments. Similarly, no differences were found on growth performance, nutrient digestibility, and carcass characteristics of pigs during the finishing period among dietary treatments. As a result of the beta diversity analysis, microbial populations were not clustered between CON and GBR100 during the growing phase, but clustered into two distinct groups of CON and GBR100 during the finishing phase. In conclusion, brown rice can be added to the diets of growing-finishing pigs by replacing corn up to 100% without negatively affecting growth performance of the pigs; additionally, this may have an effect on changes in pig intestinal microbiota if continued for a long time.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 77-78
Author(s):  
Yi-Chi Cheng ◽  
Marcos E Duarte ◽  
Sung Woo Kim

Abstract The objective was to determine the functional and nutritional values of Corynebacterium glutamicum Cell Mass (CGCM) on growth performance and gut health of newly-weaned pigs. Forty newly-weaned pigs (21 d of age; initial BW 7.1 ± 0.4 kg) were allotted to 5 dietary treatments based on randomized complete block design with sex and BW as blocks. The lysine broth of CGCM (CJ Bio, Fort Dodge, IA) was homogenized by using French press and dried to obtain lysed CGCM. Dietary treatments were: basal diet with lysed CGCM at 0, 0.7, 1.4, 2.1%, and with 1.4% intact CGCM. Experimental diets were formulated based on nutrient requirements (NRC, 2012) and pigs were fed based on 2 phases (10 and 11 d for each phase). Titanium dioxide (0.4%) was added to phase 2 diets as an indigestible external marker to calculate nutrient digestibility. Feed intake and BW were measured at d 0, 10, and 21. Pigs were euthanized on d 21 to collect proximal and distal jejunal mucosa to measure TNF-α, IL-8, MDA, IgA, and IgG concentrations. Diets and ileal digesta were collected to measure AID. Data were analyzed by SAS using MIXED, REG, and GLM procedures. Overall, increasing daily lysed CGCM intake increased (P &lt; 0.05) ADG (211 to 296 g) and ADFI (432 to 501 g). Increasing levels of lysed CGCM decreased (P &lt; 0.05) MDA and changed (quadratic, P &lt; 0.05) IgA (max: 4.90 ng/mg at 1.13%) and IgG (max: 3.37 ng/mg at 1.04%) in the proximal jejunal mucosa. Increasing daily lysed CGCM intake had quadratic effect (P&lt; 0.05) of protein carbonyl (max: 6.3 μmol/mg at 4.9 g/d). Lysed CGCM potentially benefits growth performance and gut health of newly-weaned pigs by reducing oxidative stress and increasing immune response.


2021 ◽  
Vol 41 (1) ◽  
pp. 91-99
Author(s):  
A. A. Malik ◽  
M. J. Ibrahim ◽  
Y. S. Kudu ◽  
O. P. Jibogun ◽  
Y. J. Adeniyi ◽  
...  

A nine (9) weeks feeding trial was conducted on seventy two (72) day-old Indigenous turkey poults raised on deep litter to determine their growth performance, carcass and economic characteristics when fed three dietary treatments (T1, T2, and T3, containing 0%, 25% and 50% replacement of maize with millet respectively. The birds were divided into three replicates of 8 birds per replicate; making a total of 24 birds per treatment. At the end of the & week, 4 birds per replicate were transferred to specially-designed metabolism cages for a digestibility study involving the collection of faecal droppings; while at the end of the week of the experiments, two birds per replicate were randomly selected and slaughtered to determine their carcass characteristics. Turkey poults fed millet at 25 %replacement showed significantly (p<0.05) higher body weight gain, lower cost of feed per kg live weight gain and higher revenue generated per bird; but there were no significant (p>0.05) differences in the performance of birds on 0% and 50 % replacements for those parameters. Also, there were no significant (p>0.05) differences in feed intake and mortality across the treatments. Similarly, there were no significant (p>0.05) differences in nutrient digestibilities and carcass characteristics of the birds among the various dietary treatments. Therefore, it can be concluded hat millet cam be used to replace maize up to S0 % in the diets of turkey poults at the starter phase with no detrimental effects on their performance


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 195-196
Author(s):  
Vetriselvi Sampath ◽  
Hyun Ju Park ◽  
Inho Kim ◽  
Huan Wang ◽  
Raihanul Hoque

Abstract The study was conducted to assess the effect of black pepper (BP) supplementation on the growth performance, nutrient digestibility, fecal microbial, fecal gas emission, and meat quality of finishing pigs. A total of 180 crossbred [(Landrace × Yorkshire) × Duroc] finishing pigs with average initial body weight (BW) of 53.72 ± 1.42 kg were used in 10-week trial and allotted to 6 dietary treatments (6 replications pens/treatment with 5 pigs per pen). The dietary treatments were: CON (basal diet), TRT1- CON + 0.025% BP, TRT2- CON + 0.05% BP, TRT3- CON + 0.1% BP, TRT4- CON + 0.2% BP, TRT5- CON + 0.4% BP. A linear increase (P = 0.0380, 0.0061) in body weight gain (BWG) and average daily gain (ADG) were observed respectively, during the overall trial in pigs fed BP supplemented diet compared to control. The dietary supplementation of BP showed a linear increase (P=0.0065) in gain and feed ratio (G: F) at week 10. However, there were no significant results observed on average daily feed intake (ADFI) during the overall experiment. The total tract digestibility of dry matter (DM) was linearly improved (P=0.0531) in BP treatment groups compared to control. In addition, BP diet supplementation had linearly increased fecal Lactobacillus counts (P=0.0482) and decreased E. coli counts (P=0.0306) in pigs at week 10. Furthermore, NH3, methyl mercaptans, and acetic acid was linearly decreased (P=0.0227, 0.0555,0.0541) in pigs fed BP supplementation compare to control. The inclusion of BP supplementation in pigs diet had linearly increased (P=0.0146) the backfat thickness at week 10. Thus, we concluded that BP supplementation had positively enhanced the growth performance, nutrient digestibility, fecal microbial, fecal gas emission, and meat quality of finishing pigs.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 118-118
Author(s):  
Yi-Chi Cheng ◽  
Marcos E Duarte ◽  
Sung Woo Kim

Abstract The objective was to evaluate Yarrowia lipolytica (YL) as a lipid supplement fed to nursery pigs for the growth performance and apparent ileal digestibility (AID). Twenty-four pigs weaned at 21 d of age (12 barrows and 12 gilts with initial BW at 7.2 ± 0.6 kg) were allotted to 3 dietary treatments (n = 8) based on the randomized complete block design with sex and BW as blocks. The main effect was the dietary supplementation of YL (0, 1.5, and 3.0%, replacing poultry fat) with nutrients meeting or exceeding the requirements by NRC (2012) and fed to pigs for 21 d based on 2 phases. Feed intake and BW were recorded at d 0, 10, and 21 to calculate ADG, ADFI, and G:F. Fecal scores were recorded at every odd day from d 3 to 19. Pigs were euthanized on d 21 to collect ileal digesta for AID of nutrients in diets. Data were analyzed using Proc Mixed of SAS. There were no differences in growth performance among treatments. Whereas, pigs fed 0 and 1.5% YL had higher (P &lt; 0.05) AID of DM (55.0 and 52.2% to 40.2%, respectively) and GE (62.1 and 60.6% to 49.8%, respectively) than pigs fed 3% YL. There were no differences in AID of DM and GE between the pigs fed 0% and 1.5% YL. In conclusion, 1.5% Yarrowia lipolytica could be supplemented in nursery pig diets reducing 0.8% of poultry fat without affecting growth performance and AID of DM and GE.


2021 ◽  
Vol 44 (3) ◽  
pp. 354-365
Author(s):  
B. O. Alabi ◽  
T. O. Ososanya

Crude protein is essentially needed for ruminants but expensive to supply. Mucuna puriens is a high yielding domesticated legume. When the legume is strategically combined with a fibre source, it will make a good diet for ruminants. Thus, a study was carried out to assess the effect of ensiling mucuna with maize forage on performance of rams. In the study, rams were fed silage combinations of Maize Forage (MF) and Mucuna pruriens foliage (MPF). Downy mildew and streak resistant (DMR-SR) yellow variety maize was planted and harvested as MF at six weeks of growth and ensiled with MPF at four levels : 1:0, 3:1, 1:1 and 1:3 for 21 days. All the four silages were fed to 20 WAD rams (12.00±0.25 Kg) in a completely randomised design for 105 days. Proximate composition of the silages, apparent nutrient digestibility of silages in WAD rams, feed intake and weight changes were determined using standard procedures, while feed conversion ratio was computed using standard procedures. Data obtained were analysed using descriptive statistics and ANOVA at α0.05...The dry matter (DM) was significantly higher in T1 (25.65±0.62) and T2 (23.48±0.47) than T3 (21.80±0.41) and T4 (21.57±0.40). The crude protein (CP) was highest in T4 (13.0±0.6) and least in T1 (8.0±0.2). The crude fibre was significantly higher in T1 (26.4±2.6) and T2 (26.2±2.0) than T3 (24.0±1.8) and T4 (21.6±0.9). Apparent digestibility of neutral detergent fibre (NDF) was highest in T2 (59.2±1.7) and least in T4 (37.7±1.9), while percentage nitrogen retention was significantly higher in T2 (43.2±3.8) and lower in T1 (20.0±4.3). The FCR for rams fed T2 was significantly lower (8.84±1.2) than T1 (9.76±0.80), T3 (9.60 ±0.60) and T4 (11.50±0.90) Ensiling maize forage with Mucuna pruriens foliage at 3:1 enhanced nutrient digestibility and growth performance in West African dwarf rams without any deleterious effect.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 395-396
Author(s):  
Hansol Kim ◽  
Beob Gyun G Kim

Abstract The objectives were to determine the digestible energy, standardized ileal digestibility of AA, and growth performance of dietary spray-dried plasma protein (SDPP) in nursery pigs. In Exp. 1, twelve nursery barrows (9.8 ± 0.9 kg) were assigned to a quadruplicated 3 × 2 Latin square design with 3 diets and 2 periods. Each period consisted of 5 days of adaptation, 2 days of fecal sampling, and 2 days of ileal collection. A basal diet was composed of corn, soybean meal, dried whey, and sucrose as the sole energy and AA sources. Experimental diets were prepared by replacing 15% of the energy and AA sources in the basal diet with SDPP 1 (manufactured in the US; 78.2% CP and 4,862 kcal GE/kg as-is) or SDPP 2 (manufactured in Korea; 74.3% CP and 4,636 kcal GE/kg as-is). Spray-dried plasma protein 1 had greater digestible energy (4,799 vs. 4,469 kcal/kg as-is; P &lt; 0.05), but less (P &lt; 0.05) standardized ileal digestibility of lysine, methionine, tryptophan, and threonine compared with SDPP 2. In Exp. 2, eighty-four nursery pigs (7.9 ± 0.7 kg) were allotted to 3 dietary treatments in a randomized complete block design with 7 replicate pens and 4 pigs per pen. Three corn-soybean meal-whey-based diets contained fish meal (6% and 3.5% for d 0 to 14 and d 14 to 28, respectively), SDPP 1 (4.5% and 2.7%), or SDPP 2 (5% and 3%) to maintain same energy and nutrient concentrations. During d 0 to 14 and overall period, pigs fed the diets containing SDPP gained more weight (P &lt; 0.05) than those fed the fish meal diet with no difference between 2 sources of SDPP. Overall, 2 sources of SDPP have different energy concentrations and AA digestibility, but similar growth promoting effects in nursery pigs.


Author(s):  
Nan Zhang ◽  
Xiaoming Song ◽  
Wenxuan Dong ◽  
Ling Liu ◽  
Zhiying Cui ◽  
...  

Abstract This study was conducted to determine the digestible energy (DE), metabolizable energy (ME), and ileal digestibility of amino acids (AA) of fish protein hydrolysate (FPH), and to evaluate the effects of FPH on the performance of piglets. In Exp. 1, the available energy content of FPH was determined by difference methods: 12 barrows with an initial body weight (BW) of 40.0±2.1kg were randomly assigned into two dietary treatments with six replicates per treatment, the treatments contained a corn basal diet and an experimental diet in which 20% corn was replaced by FPH. The experiment was lasted for 12 days, with 7 days adaptation period followed by 5 days total collection of feces and urine. In Exp. 2, the standardized ileal digestibility (SID) of AA in FPH by the N-free method was determined, 12 barrows (initial BW of 35.3±1.8 kg) with ileal T-cannulas were randomly allotted into two treatments with six replicates per treatment, the dietary treatments consisted of an experimental diet and a N-free diet. The experiment was lasted for 7 days, with 5 days adaptation period followed by 2 days collection of ileal digesta. In Exp. 3, the effect of FPH on the performance of piglets was conducted. 192 piglets (initial BW of 8.10±1.8kg) were randomly allotted into four treatments with six replicates per treatment. The treatments were a control diet with fish meal (FM) and part or all FM was replaced by FPH, got diets containing 2%, 3% or 5% FPH. The experiment lasted for 28 days. Results showed that: In Exp. 1, the DE and ME of FPH were 21.12MJ/kg and 20.28MJ/kg. In Exp. 2, the SID of Lys, Met, Thr and Trp were 79.99%, 87.17%, 68.29%, and 71.53% respectively. In Exp. 3, addition of 3% FPH increased nutrient digestibility and volatile fatty acid content in feces. Addition of 5% FPH increased the average daily feed intake (ADFI), significantly increased (P&lt;0.05) the activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and immunoglobulin A (IgA), while significantly decreased (P&lt;0.05) the average daily gain (ADG). In conclusion, FPH had a high value of digestible energy and ileal digestible essential amino acids, could improve nutrient digestibility, immunity, antioxidant capacity, and intestinal health of piglets, while adverse to nitrogen deposition in piglets.


2020 ◽  
Vol 100 (1) ◽  
pp. 133-139
Author(s):  
Xiang Ao ◽  
Yan Lei ◽  
In Ho Kim

This study was conducted to evaluate the effect of supplementation of different flavors (apple and anise) on growth performance, nutrient digestibility, blood profiles, and carcass quality in growing–finishing pigs. A total of 96 growing pigs [(Yorkshire × Landrace) × Duroc] with an average body weight (BW) of 28.2 ± 0.7 kg were randomly assigned to one of the following three treatments: (1) CON, basal diet; (2) APF, basal diet + 0.05% apple flavor; (3) ANF, basal diet + 0.05% anise flavor, according to their BW and sex in this 15 wk experiment. There were eight replications (pens) per treatment and four pigs per pen (two barrows and two gilts). During week 0–5, pigs fed ANF diets had greater (p < 0.05) average daily gain (ADG) and average daily feed intake than those fed CON and APF diets. Dietary ANF treatment increased (p < 0.05) ADG during 0–15 wk compared with CON treatment. At the end of 5 wk, the apparent total tract digestibility of nitrogen in ANF treatment was improved (p < 0.05) compared with that in CON treatment. Dietary treatments did not affect the studied traits of carcass and meat quality. The inclusion of anise flavor increased ADG, but apple flavor had no effect on growth performance in growing–finishing pigs.


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