Clostridium difficile Exposures, Colonization, and the Microbiome: Implications for Prevention

2018 ◽  
Vol 39 (5) ◽  
pp. 596-602 ◽  
Author(s):  
Sara L. Revolinski ◽  
L. Silvia Munoz-Price
Keyword(s):  
Clostridium Difficile ◽  
High Throughput ◽  
Environmental Contamination ◽  
Gut Microbiome ◽  
High Throughput Sequencing ◽  
Infect Control Hosp ◽  
Healthcare Settings ◽  
Potential Impact

New studies have been published regarding the epidemiology of Clostridium difficile in topics such as asymptomatic C. difficile colonization, community-associated C. difficile infection, environmental contamination outside healthcare settings, animal colonization, and the interactions between C. difficile and the gut microbiome. In addition to summarizing these findings, this review offers a perspective on the potential impact of high-throughput sequencing and other potential techniques on the prevention of C. difficile.Infect Control Hosp Epidemiol 2018;39:596–602

scholarly journals Relationship between hyperlipidemia and the gut microbiome of rats, characterized using high-throughput sequencing

2020 ◽  
Vol 7 (2) ◽  
pp. 154-161 ◽  
Author(s):  
Yaqi Li ◽  
Quantao Ma ◽  
Jingkang Wang ◽  
Pengfei Li ◽  
Long Cheng ◽  
...  
Keyword(s):  
High Throughput ◽  
Gut Microbiome ◽  
High Throughput Sequencing

scholarly journals High‐throughput sequencing reveals the core gut microbiome of Bar‐headed goose ( Anser indicus ) in different wintering areas in Tibet

2016 ◽  
Vol 5 (2) ◽  
pp. 287-295 ◽  
Author(s):  
Wen Wang ◽  
Jian Cao ◽  
Fang Yang ◽  
Xuelian Wang ◽  
Sisi Zheng ◽  
...  
Keyword(s):  
High Throughput ◽  
Gut Microbiome ◽  
High Throughput Sequencing ◽  
The Core ◽  
Wintering Areas ◽  
Anser Indicus

Skin and Environmental Contamination in Patients Diagnosed With Clostridium difficile Infection but Not Meeting Clinical Criteria for Testing

2015 ◽  
Vol 36 (11) ◽  
pp. 1348-1350 ◽  
Author(s):  
Sirisha Kundrapu ◽  
Venkata Sunkesula ◽  
Myreen Tomas ◽  
Curtis J. Donskey
Keyword(s):  
Clostridium Difficile ◽  
Clostridium Difficile Infection ◽  
Environmental Contamination ◽  
Infect Control Hosp ◽  
Antibiotic Exposure ◽  
Clinical Criteria

Of 134 patients diagnosed with Clostridium difficile infection, 30 (22%) did not meet clinical criteria for testing because they lacked significant diarrhea or had alternative explanations for diarrhea and no recent antibiotic exposure. For these patients, skin and/or environmental contamination was common only in those with prior antibiotic exposure.Infect. Control Hosp. Epidemiol. 2015;36(11):1348–1350

scholarly journals Evaluation on the Characteristics of Gut Microbiome in Polycystic Ovary Syndrome Rats Induced by Dihydrotestosterone or Letrozole

2020 ◽  
Author(s):  
Yanhua Zheng ◽  
Hongxia Ma ◽  
Ying Xu ◽  
Chengjie Liang ◽  
Tong Yang
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Gut Microbiota ◽  
Aromatase Inhibitor ◽  
16S Rdna ◽  
High Throughput ◽  
Gut Microbiome ◽  
High Throughput Sequencing ◽  
Polycystic Ovary ◽  
Molecular Ecology ◽  
Ovary Syndrome

Abstract The etiology of polycystic ovary syndrome (PCOS) is unclear. Recent reports indicated that the gut microbiome of PCOS patients and rodents has changed. In this study, we induced the nonaromatizable androgen dihydrotestosterone (DHT) or the aromatase inhibitor letrozole (LET) to induce PCOS model rat to compare the bacterial diversity distribution within and between the two groups. The molecular ecology of the fecal gut microbiota was analyzed by 16S rDNA high-throughput sequencing. Our study found that DHT can reduce the microbial richness in rats. PCoA plots confirmed that DHT group was statistically significantly separated from C group and LET group. At phylum level, DHT led to a decrease in Bacteroidetes as well as an increase in Cyanobacteria, Tenericutes, Actinobacteria, Spirochaetae and Saccharibacteria. At genus level, LEfSe analysis showed that genus of Bifidobacteriales, Vibro, Peptococcus and Turicibacter played roles in the letrozole induced PCOS rats. And Lachnospiraceae_NK4A136_group, Ruminococcus_1, Ruminiclostridium, Anaerotruncus and Anaeroplasma played vital roles in the intestine of DHT induced PCOS rats.

scholarly journals Gut Microbiome Composition in Obese and Non-Obese Persons: A Systematic Review and Meta-Analysis

Nutrients ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 12
Author(s):  
Mariona Pinart ◽  
Andreas Dötsch ◽  
Kristina Schlicht ◽  
Matthias Laudes ◽  
Jildau Bouwman ◽  
...  
Keyword(s):  
Systematic Review ◽  
High Throughput ◽  
Gut Microbiome ◽  
High Throughput Sequencing ◽  
Meta Analysis ◽  
Shannon Index ◽  
Obese Adults ◽  
Cross Sectional ◽  
Microbiome Composition ◽  
Genus Level

Whether the gut microbiome in obesity is characterized by lower diversity and altered composition at the phylum or genus level may be more accurately investigated using high-throughput sequencing technologies. We conducted a systematic review in PubMed and Embase including 32 cross-sectional studies assessing the gut microbiome composition by high-throughput sequencing in obese and non-obese adults. A significantly lower alpha diversity (Shannon index) in obese versus non-obese adults was observed in nine out of 22 studies, and meta-analysis of seven studies revealed a non-significant mean difference (−0.06, 95% CI −0.24, 0.12, I2 = 81%). At the phylum level, significantly more Firmicutes and fewer Bacteroidetes in obese versus non-obese adults were observed in six out of seventeen, and in four out of eighteen studies, respectively. Meta-analyses of six studies revealed significantly higher Firmicutes (5.50, 95% 0.27, 10.73, I2 = 81%) and non-significantly lower Bacteroidetes (−4.79, 95% CI −10.77, 1.20, I2 = 86%). At the genus level, lower relative proportions of Bifidobacterium and Eggerthella and higher Acidaminococcus, Anaerococcus, Catenibacterium, Dialister, Dorea, Escherichia-Shigella, Eubacterium, Fusobacterium, Megasphera, Prevotella, Roseburia, Streptococcus, and Sutterella were found in obese versus non-obese adults. Although a proportion of studies found lower diversity and differences in gut microbiome composition in obese versus non-obese adults, the observed heterogeneity across studies precludes clear answers.

scholarly journals Investigation of gut microbiome changes in type 1 diabetic mellitus rats based on high-throughput sequencing

2020 ◽  
Vol 124 ◽  
pp. 109873 ◽  
Author(s):  
Quantao Ma ◽  
Yaqi Li ◽  
Jingkang Wang ◽  
Pengfei Li ◽  
Yuhui Duan ◽  
...  
Keyword(s):  
High Throughput ◽  
Gut Microbiome ◽  
High Throughput Sequencing ◽  
Diabetic Mellitus

scholarly journals High-Throughput Sequencing Reveals the Gut Microbiome of the Bactrian Camel in Different Ages

2019 ◽  
Vol 76 (7) ◽  
pp. 810-817 ◽  
Author(s):  
Jing He ◽  
Le Hai ◽  
Khongorzul Orgoldol ◽  
Li Yi ◽  
Liang Ming ◽  
...  
Keyword(s):  
High Throughput ◽  
Gut Microbiome ◽  
High Throughput Sequencing ◽  
Bactrian Camel ◽  
Different Ages

scholarly journals Comparison of four high throughput sequencing platforms in a medical laboratory for gut microbiome research

2020 ◽  
Vol 59 (2-Spl) ◽  
pp. 89-99
Author(s):  
P. DAS ◽  
R. DADWAL ◽  
V. RADHAKRISHNAN ◽  
M. PARIHAR ◽  
S. BHATTACHARYA ◽  
...  
Keyword(s):  
High Throughput ◽  
Gut Microbiome ◽  
High Throughput Sequencing ◽  
Medical Laboratory ◽  
Microbiome Research ◽  
Sequencing Platforms
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