scholarly journals Hospital microbiology laboratory practices for Enterobacteriaceae: Centers for Disease Control and Prevention National Healthcare Safety Network (NHSN) annual survey, 2015 and 2016

2018 ◽  
Vol 39 (9) ◽  
pp. 1115-1117 ◽  
Author(s):  
Alicia Shugart ◽  
Maroya Spalding Walters ◽  
Lindsey M. Weiner ◽  
David Lonsway ◽  
Alexander J. Kallen
Keyword(s):  
Susceptibility Testing ◽  
Multidrug Resistant ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
National Healthcare ◽  
Annual Survey ◽  
National Healthcare Safety Network ◽  
Control And Prevention ◽  
Using Data ◽  
Facility Survey

AbstractWe analyzed clinical microbiology laboratory practices for detection of multidrug-resistant Enterobacteriaceae in US short-stay acute-care hospitals using data from the National Healthcare Safety Network (NHSN) Annual Facility Survey. Half of hospitals reported testing for carbapenemases, and 1% performed routine polymyxin susceptibility testing using reference broth microdilution.

scholarly journals Policies for Controlling Multidrug-Resistant Organisms in US Healthcare Facilities Reporting to the National Healthcare Safety Network, 2014

2016 ◽  
Vol 37 (9) ◽  
pp. 1105-1108 ◽  
Author(s):  
Lindsey M. Weiner ◽  
Amy K. Webb ◽  
Maroya S. Walters ◽  
Margaret A. Dudeck ◽  
Alexander J. Kallen
Keyword(s):  
Acute Care ◽  
Multidrug Resistant ◽  
Healthcare Facilities ◽  
Contact Precautions ◽  
National Healthcare ◽  
National Healthcare Safety Network ◽  
Chlorhexidine Bathing ◽  
Multidrug Resistant Organisms ◽  
Using Data ◽  
Facility Survey

We examined reported policies for the control of common multidrug-resistant organisms (MDROs) in US healthcare facilities using data from the National Healthcare Safety Network Annual Facility Survey. Policies for the use of Contact Precautions were commonly reported. Chlorhexidine bathing for preventing MDRO transmission was also common among acute care hospitals.Infect Control Hosp Epidemiol 2016:1–4

scholarly journals Changes in the Number of Intensive Care Unit Beds in U.S. Hospitals During the Early Months of the COVID-19 Pandemic, as reported to the National Healthcare Safety Network’s COVID-19 Module

2021 ◽  
pp. 1-12
Author(s):  
Lindsey M. Weiner-Lastinger ◽  
Margaret A. Dudeck ◽  
Katherine Allen-Bridson ◽  
Raymund Dantes ◽  
Cindy Gross ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Hospital Type ◽  
National Healthcare ◽  
National Healthcare Safety Network ◽  
Bed Capacity ◽  
Using Data

Abstract Using data from the National Healthcare Safety Network (NHSN), we assessed changes to intensive care unit (ICU) bed capacity during the early months of the COVID-19 pandemic. Changes in capacity varied by hospital type and size. ICU beds increased by 36%, highlighting the pressure placed on hospitals during the pandemic.

scholarly journals Validation of Sensititre Dry-Form Broth Microdilution Panels for Susceptibility Testing of Ceftazidime-Avibactam, a Broad-Spectrum-β-Lactamase Inhibitor Combination

2015 ◽  
Vol 59 (8) ◽  
pp. 5036-5039 ◽  
Author(s):  
Ronald N. Jones ◽  
Nicole M. Holliday ◽  
Kevin M. Krause
Keyword(s):  
Broad Spectrum ◽  
Susceptibility Testing ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Broth Microdilution ◽  
Content Type ◽  
Serious Infections ◽  
Validation Experiment ◽  
Inhibitor Combination ◽  
Lactamase Inhibitor

ABSTRACTCeftazidime-avibactam is a broad-spectrum-β-lactamase inhibitor combination in late-stage clinical development for the treatment of serious infections. In preparation for clinical microbiology laboratory use, a validation experiment was initiated to evaluate a commercial broth microdilution product (Sensititre dried MIC susceptibility system) compared to reference panels using 525 recent clinical isolates. Among 11 pathogen groups, all had Sensititre MIC/reference MIC ratios predominantly at 1 (47.5% to 97.5%), and automated and manual endpoint results did not differ.EnterobacteriaceaeMIC comparisons showed a modest skewing of Sensititre MIC results toward an elevated MIC (33.9%), but the essential agreement was 98.9% with 100.0% reproducibility. In conclusion, Sensititre panels produced accurate ceftazidime-avibactam MIC results, allowing quality MIC guidance for therapy following regulatory approvals.

scholarly journals Surveying the Surveillance: Surgical Site Infections Excluded by the January 2013 Updated Surveillance Definitions

2014 ◽  
Vol 35 (5) ◽  
pp. 570-573 ◽  
Author(s):  
Kristen V. Dicks ◽  
Sarah S. Lewis ◽  
Michael J. Durkin ◽  
Arthur W. Baker ◽  
Rebekah W. Moehring ◽  
...  
Keyword(s):  
Disease Control ◽  
Surgical Site Infections ◽  
National Healthcare ◽  
National Healthcare Safety Network ◽  
Control And Prevention ◽  
Centers For Disease Control

The updated 2013 Centers for Disease Control and Prevention/National Healthcare Safety Network definitions for surgical site infections (SSIs) reduced the duration of prolonged surveillance from 1 year to 90 days and defined which procedure types require prolonged surveillance. Applying the updated 2013 SSI definitions to cases analyzed using the pre-2013 surveillance definitions excluded 10% of previously identified SSIs.

scholarly journals Antimicrobial Stewardship Practices Reported by California Hospitals Following New Legislative Requirements: Analysis of National Healthcare Safety Network Annual Survey Data, 2014-2015

2017 ◽  
Vol 38 (12) ◽  
pp. 1503-1505 ◽  
Author(s):  
Kyle Rizzo ◽  
Melissa Kealey ◽  
Erin Epson
Keyword(s):  
Survey Data ◽  
Antimicrobial Stewardship ◽  
Requirements Analysis ◽  
Antimicrobial Stewardship Program ◽  
National Healthcare ◽  
Annual Survey ◽  
National Healthcare Safety Network ◽  
Stewardship Program ◽  
Core Elements ◽  
Implementation Progress

We analyzed California hospitals’ National Healthcare Safety Network Annual Surveys to describe antimicrobial stewardship program (ASP) implementation progress following new state ASP legislation. The proportion of hospitals with all 7 Core Elements of Hospital ASP rose from 59.3% in 2014 to 69.2% in 2015 (P<.001).Infect Control Hosp Epidemiol 2017;38:1503–1505

scholarly journals Real life turnaround time of blood cultures in the clinical microbiology laboratory: results of the first Italian survey, May 2015

2016 ◽  
Vol 31 (3) ◽  
Author(s):  
Fabio Arena ◽  
Marta Argentieri ◽  
Paola Bernaschi ◽  
Giacomo Fortina ◽  
Vesselina Kroumova ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Susceptibility Testing ◽  
Real Life ◽  
Turnaround Time ◽  
High Rate ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Italian Survey ◽  
Multiple Outcome ◽  
Reported Data

<em>Background and aims</em>: Blood culture (BC) results are essential to guide antimicrobial chemotherapy for patients with sepsis. However, BC is a time-consuming exam, which can take several days. Reducing BCs turn around time (TAT) could impact on multiple outcome parameters and TAT monitoring is an important tool for measurement of microbiology laboratory performance. The aim of this study was to provide an overview of BC TATs among Italian microbiology laboratories. <br /><em>Materials and methods</em>: Five laboratories collected and recorded, for a month period, date and time of the BC processing events. Cumulative TATs were analysed using the GraphPad software. <br /><em>Results</em>: Participating laboratories reported data from 302 sepsis episodes. The median time from when the BC system produced a positive signal until Gram-stain results were reported was 7.6 hours. A rapid molecular identification and antimicrobial susceptibility testing (AST) was performed in 26.5% of BCs. Mean TAT for identification report was significantly lower when a molecular approach was adopted (12 vs. 28.7 hours, P&lt;0.001). Similarly, results of the molecular AST were obtained more than 24 hours in advance compared with phenotypic AST (mean 13.2 vs. 47.6, P&lt;0.001). TATs from BC positivity of laboratories opened 7 days/week were not significantly lower than those of laboratories opened 6 days/week. <br /><em>Conclusions</em>: BC is a time-consuming exam, however, molecular identification and AST methods can drastically reduce time to results. The lack of difference between TATs observed for laboratories working 7 days/week and 6 days/week, coupled with a high rate of BCs turning positive during the night enable to conclude that the most urgent measure to reduce TATs is the expansion of laboratory regular duty hours.

scholarly journals Evaluation of the Oxyrase OxyPlate Anaerobe Incubation System

2000 ◽  
Vol 38 (2) ◽  
pp. 499-507 ◽  
Author(s):  
Lois S. Wiggs ◽  
Joseph J. Cavallaro ◽  
J. Michael Miller
Keyword(s):  
Clinical Microbiology ◽  
Anaerobic Bacteria ◽  
Clinical Microbiology Laboratory ◽  
Reference Laboratory ◽  
Coding System ◽  
Microbiology Laboratory ◽  
Bacterial Strains ◽  
Incubation System ◽  
Control And Prevention ◽  
Clinically Significant

The Oxyrase OxyPlate anaerobe incubation system was evaluated for its ability to support the growth of clinically significant anaerobic bacteria previously identified by the Anaerobe Reference Laboratory at the Centers for Disease Control and Prevention. The results were compared with those obtained with conventional anaerobe blood agar plates incubated in an anaerobe chamber. We tested 251 anaerobic bacterial strains. Plates were read at 24, 48, and 72 h; growth was scored by a numerical coding system that combines the degree of growth and the colony size. Organisms (number of strains tested) used in this study were Actinomyces (32),Anaerobiospirillum (8), Bacteroides(39), Campylobacter (8), Clostridium (96),Fusobacterium (12), Leptotrichia (8),Mobiluncus (8), Peptostreptococcus(16), and Propionibacterium (24). At 24 h, 101 (40.2%) of the 251 strains tested showed better growth with the anaerobe chamber than with the OxyPlate system, 10 (4.1%) showed better growth with the OxyPlate system, and the remaining 140 (55.8%) showed equal growth with both systems. At 48 h, 173 (68.9%) showed equal growth with both systems, while 78 (31.1%) showed better growth with the anaerobe chamber. At 72 h, 176 (70.1%) showed equal growth with both systems, while 75 (29.9%) showed better growth with the anaerobe chamber. The OxyPlate system performed well for the most commonly isolated anaerobes but was inadequate for some strains. These results indicate that the Oxyrase OxyPlate system was effective in creating an anaerobic atmosphere and supporting the growth of anaerobic bacteria within 72 h. OxyPlates would be a useful addition to the clinical microbiology laboratory lacking resources for traditional anaerobic culturing techniques.

scholarly journals Phenotypic classification of multidrug-resistant Klebsiella through MALDI-TOF mass spectrometry

2015 ◽  
Vol 30 (1) ◽  
Author(s):  
Anna Di Taranto ◽  
Rosella De Nittis ◽  
Marco Fagioni ◽  
Tiziana Rollo ◽  
Paola Cella ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Multidrug Resistant ◽  
Clinical Microbiology Laboratory ◽  
Laser Desorption Ionization ◽  
Microbiology Laboratory ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Microbial Isolates

<em>Background</em>. Resistance to carbapenems in <em>Klebsiella</em> <em>pneumoniae</em> infections could compromise therapy efficiency in hospitals. In Italy, the percentage of <em>K</em>. <em>pmeumoniae</em> resistant to carbapenems blood-isolated is 35%. <br /><em>Materials</em> <em>and</em> <em>Methods</em>. Seventy-three multidrug-resistant <em>K</em>. <em>pneumoniae</em> clinical isolates coming from Foggia (Italy) have been phenotypically classified through matrix assisted laser desorption/ionization-time of flight mass spectrometry between February and May 2014 in order to locate an eventual infectious outbreak. <br /><em>Conclusions</em>. Classification of microbial isolates is essential to this purpose and starts from phenotypic methodologies to the most complex genotypic methods. Among these, mass spectrometry utilization appears to be a simple, useful and quick instrument in a clinical microbiology laboratory.

Sign in / Sign up

Export Citation Format

Share Document