Just the Facts: Diagnosis and management of rhabdomyolysis

CJEM ◽  
2020 ◽  
Vol 22 (6) ◽  
pp. 745-748
Author(s):  
Brit Long ◽  
Elisha Targonsky ◽  
Alex Koyfman
Keyword(s):  
Creatine Kinase ◽  
Physical Examination ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Exercise Program ◽  
Diagnosis And Management ◽  
Dark Urine ◽  
Muscle Tenderness

A 32-year-old male presents with diffuse myalgias, weakness, and dark urine for 1 day. The patient states he recently started a new exercise program. He is hemodynamically stable, and his physical examination reveals diffuse muscle tenderness. His creatine kinase (CK) returns at 8,000 international units per liter (IU/L), and his urinalysis reveals blood but only three red blood cells (RBCs) on microscopy.

scholarly journals STUDI KASUS : PROFIL SEL DARAH MERAH ANJING YANG TERINFEKSI BABESIA SP.

2018 ◽  
Vol 6 (1) ◽  
pp. 12-16
Author(s):  
Yanse Yane Rumlaklak ◽  
Jois Moriani Jacob ◽  
Aven B Oematan
Keyword(s):  
Physical Examination ◽  
Red Blood Cells ◽  
Clinical Examination ◽  
Blood Cells ◽  
Clinical Symptoms ◽  
Hemoglobin Concentration ◽  
Blood Parasites ◽  
Blood Collection ◽  
Laboratory Examination ◽  
Blood Tests

Babesia sp. is a protozoa that infects dogs through tick bite and can cause babesiosis. The purpose of blood tests in case dogs is to find out the profile of the dog's red blood cells with babesiosis. On clinical examination found many ticks on the dog's body. Dogs show clinical symptoms of dehydration and paleness in the mucosa. Blood collection is done through the anterior antibrachii cephalica vein. Blood is taken and collected in EDTA tubes. Then the examination is done by examining blood parasites, examining erythrocyte values, examining hemoglobin concentration and hematocrit values. Examination of blood parasites was done by making reticulocyte preparations, while examining erythrocyte values, hemoglobin concentration and hematocrit values were performed using a Vet Scan® HM 5TM machine. The results of examination of reticulocyte preparations showed the existence of Babesia sp infestations with a presentation of 0.68%. Erythrocyte values, hemoglobin concentration and hematocrit values were 3.52x106 // µL, 6.90 g / dL and 23.81%. Based on history, physical examination, clinical and laboratory examination, it can be concluded that the Beagle dog named Loh has microcytic hypochromatic anemia.

scholarly journals Effect of Self-Controlled Exercise on Antioxidant Activity of Red Blood Cells and Functional Recovery of Limbs in Patients with Breast Cancer after Rehabilitation

2021 ◽  
Author(s):  
Feifei LI ◽  
Wei LIU ◽  
Fei HUO ◽  
Weifang HE ◽  
Fan YANG ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Antioxidant Activity ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Intervention Group ◽  
Exercise Program ◽  
Clinical Work ◽  
Control Group ◽  
Breast Cancer Patients ◽  
Postoperative Rehabilitation

Background: We aimed to investigate the effect of self-controlled exercise on the antioxidant activity of red blood cells and the recovery of limb function in patients with breast cancer after rehabilitation. Methods: Overall 130 breast cancer patients admitted to the First Affiliated Hospital of Zhengzhou University, China from Feb 2018 to Jan 2019 were divided into intervention group and control group. The control group received perioperative care and chemotherapy, the intervention group implemented a self-controlled exercise program. Indexes were compared between the two groups before intervention, 3 months and 6 months after intervention. Results: The activity of erythrocyte superoxide dismutase (SOD) in the intervention group was significantly increased in the first 3 months (P=0.030), and decreased from 3rd to 6th month (P=0.033). The glutathione peroxidase (GSH-Px) activity in the intervention group increased through the whole intervention period. The plasma malondialdehyde (MDA) in the intervention group was significantly decreased (P=0.029, 0.012). After intervention for 3 months and 6 months, the 6MND distances in the intervention group were significantly longer (P=0.001, 0.045). The average exercise time in the intervention group were significantly increased (P=0.004, 0.000). Conclusion: Self-controlled exercise can effectively improve the antioxidant ability of red blood cells in patients with breast cancer, improve the mobility of shoulder joints of the affected side and increase their exercise capacity, with good sustainability. It has positive effect on postoperative rehabilitation, could be used in long-term regular clinical work.

Creatine kinase is required for swelling-activated K-Cl cotransport in dog red blood cells

1995 ◽  
Vol 268 (3) ◽  
pp. C660-C668 ◽  
Author(s):  
G. C. Colclasure ◽  
J. C. Parker ◽  
P. B. Dunham
Keyword(s):  
Creatine Kinase ◽  
Red Blood Cells ◽  
Dose Response ◽  
Blood Cells ◽  
Creatine Phosphate ◽  
Red Cell ◽  
Essential Function ◽  
Resealed Ghosts ◽  
A Site

K-Cl cotransport in resealed dog red cell ghosts requires the incorporation of creatine phosphate before resealing; incorporation of ATP has no effect [Colclasure and Parker. Am. J. Physiol. 265 (Cell Physiol. 34): C1648-C1652, 1993]. A role for creatine kinase (CK) in swelling-activated K-Cl cotransport was investigated. 2,4-Dinitrofluorobenzene (DNFB), an inhibitor of CK, inhibited K-Cl cotransport in intact red blood cells and resealed ghosts from DNFB-treated cells. Incorporation of exogenous CK into ghosts of DNFB-treated cells restored K-Cl cotransport. Therefore DNFB inhibits CK and not the cotransporter. Inhibition of native CK in ghosts by DNFB and the incorporation of CK into the ghosts were demonstrated in electrophoretic gels. In a dose-response experiment, approximately 770 molecules CK/ghost restored 50% of control cotransport. Since creatine phosphate is a substrate only for CK, CK provides ATP to a site inaccessible to cytoplasmic ATP. The nature of this site and its role in K-Cl cotransport are uncertain, but an essential function for CK is established.

Reduced level of creatine kinase in the plasma of Holstein cows supplemented with d-α-tocopherol acetate

1998 ◽  
Vol 78 (1) ◽  
pp. 147-149
Author(s):  
M. V. Simpson ◽  
M. Hidiroglou ◽  
T. R. Batra ◽  
X. Zhao
Keyword(s):  
Creatine Kinase ◽  
Vitamin E ◽  
Red Blood Cells ◽  
Key Words ◽  
Blood Cells ◽  
Post Partum ◽  
Holstein Cows ◽  
Tocopherol Acetate ◽  
Plasma Creatine Kinase ◽  
Key Words Vitamin E

The effects of daily supplementation of 1000 IU of either d-α-tocopherol acetate or dl-α-tocopherol acetate to Holstein cows from drying off to 8 wk post-partum on plasma creatine kinase and selenium levels in plasma and red blood cells were studied. Supplementation with either d-α-tocopherol acetate or dl-α-tocopherol acetate showed no effect on selenium levels in both plasma and red blood cells. However, supplementation with d-α-tocopherol acetate, but not dl-α-tocopherol acetate decreased plasma creatine kinase significantly (P < 0.05) prior to parturition. Key words: Vitamin E, creatine kinase, selenium

Anemia in the Newborn Infant

1984 ◽  
Vol 6 (5) ◽  
pp. 131-138
Author(s):  
Joseph D. Dickerman
Keyword(s):  
Physical Examination ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Hemoglobin Concentration ◽  
Term Infant ◽  
Laboratory Studies ◽  
Bone Marrow Aplasia ◽  
Anemia Of Prematurity ◽  
Disease Entities ◽  
The Many

The proper management of anemia in the newborn period, as at any age, requires an accurate diagnosis. It is helpful to divide the many possible etiologies into three major categories: blood loss, increased destruction of red blood cells (hemolysis), and decreased production of red blood cells (bone marrow aplasia or hypoplasia). DEFINITION The first step in the diagnosis of neonatal anemia is to define it. Normal hematologic values during the first 2 weeks of life in the term infant are presented in Table 1. Similar values for infants of low birth weight have been published by Stockman and Oski. The more common anemias will be discussed in terms of findings on physical examination, history, ry, laboratory studies, and management with reference to groups of conditions (Tables 2 and 3) rather than to specific disease entities. The less common disorders will be either omitted or described only briefly. The anemia of prematurity will not be covered. A discussion of this topic can be found in the article by Stockman. Approach to Diagnosis The usual approach to diagnosis, taking a history and then performing a physical examination, has been reversed in this paper. In most cases, a physician is called to see a neonate suspected of being anemic because of signs and/or symptoms of shock, pallor, or a low hemoglobin concentration, and the physician examines the infant prior to obtaining a history.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

1992 ◽  
Vol 50 (1) ◽  
pp. 880-881
Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Entamoeba Histolytica ◽  
Causative Agent ◽  
Blood Cells ◽  
Protozoan Parasite ◽  
Complex Phenomenon ◽  
Great Capacity ◽  
Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 914-915
Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Amelanotic Melanoma ◽  
Cytoplasmic Process ◽  
Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

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