scholarly journals 638. The Optimal Placement and Orientation of the Bipartite Two-Step Transcription Amplification (TSTA) Expression System in Adenoviral Vectors for Prostate Cancer Gene Therapy

2006 ◽  
Vol 13 ◽  
pp. S245-S246
Author(s):  
Marxa L. Figueiredo ◽  
Makoto Sato ◽  
May N. Chen ◽  
Russell Powell ◽  
Michael Carey ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Gene Therapy ◽  
Expression System ◽  
Cancer Gene Therapy ◽  
Adenoviral Vectors ◽  
Optimal Placement ◽  
Cancer Gene

scholarly journals Improving adenoviral vectors and strategies for prostate cancer gene therapy

Clinics ◽  
2018 ◽  
Vol 73 (Suppl 1) ◽  
Author(s):  
RE Tamura ◽  
IV Luna ◽  
MG Lana ◽  
BE Strauss
Keyword(s):  
Prostate Cancer ◽  
Gene Therapy ◽  
Cancer Gene Therapy ◽  
Adenoviral Vectors ◽  
Cancer Gene

scholarly journals Efficient and Selective Gene Transfer into Primary Human Brain Tumors by Using Single-Chain Antibody-Targeted Adenoviral Vectors with Native Tropism Abolished

2002 ◽  
Vol 76 (6) ◽  
pp. 2753-2762 ◽  
Author(s):  
Victor W. van Beusechem ◽  
Jacques Grill ◽  
D. C. Jeroen Mastenbroek ◽  
Thomas J. Wickham ◽  
Peter W. Roelvink ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Gene Transfer ◽  
Brain Tumors ◽  
Human Brain ◽  
Cancer Gene Therapy ◽  
Adenoviral Vectors ◽  
Receptor Expression ◽  
Normal Brain ◽  
Cancer Gene ◽  
Single Chain

ABSTRACT The application of adenoviral vectors in cancer gene therapy is hampered by low receptor expression on tumor cells and high receptor expression on normal epithelial cells. Targeting adenoviral vectors toward tumor cells may improve cancer gene therapy procedures by providing augmented tumor transduction and decreased toxicity to normal tissues. Targeting requires both the complete abolition of native tropism and the addition of a new specific binding ligand onto the viral capsid. Here we accomplished this by using doubly ablated adenoviral vectors, lacking coxsackievirus-adenovirus receptor and αv integrin binding capacities, together with bispecific single-chain antibodies targeted toward human epidermal growth factor receptor (EGFR) or the epithelial cell adhesion molecule. These vectors efficiently and selectively targeted both alternative receptors on the surface of human cancer cells. Targeted doubly ablated adenoviral vectors were also very efficient and specific with primary human tumor specimens. With primary glioma cell cultures, EGFR targeting augmented the median gene transfer efficiency of doubly ablated adenoviral vectors 123-fold. Moreover, EGFR-targeted doubly ablated vectors were selective for human brain tumors versus the surrounding normal brain tissue. They transduced organotypic glioma and meningioma spheroids with efficiencies similar to those of native adenoviral vectors, while exhibiting greater-than-10-fold-reduced background levels on normal brain explants from the same patients. As a result, EGFR-targeted doubly ablated adenoviral vectors had a 5- to 38-fold-improved tumor-to-normal brain targeting index compared to native vectors. Hence, single-chain targeted doubly ablated adenoviral vectors are promising tools for cancer gene therapy. They should provide an improved therapeutic index with efficient tumor transduction and effective protection of normal tissue.

Prostate cancer gene therapy and the role of radiation

2002 ◽  
Vol 28 (1) ◽  
pp. 49-64 ◽  
Author(s):  
J.M. Kaminski ◽  
K. Nguyen ◽  
M. Buyyounouski ◽  
A. Pollack
Keyword(s):  
Prostate Cancer ◽  
Gene Therapy ◽  
Cancer Gene Therapy ◽  
Cancer Gene

Prostate Cancer Gene Therapy

2003 ◽  
pp. 957-1009
Author(s):  
Naren R. Ramakrishna ◽  
Shidong Li ◽  
Nick Detorie ◽  
Jonathan W. Simons ◽  
Theodore L. DeWeese
Keyword(s):  
Prostate Cancer ◽  
Gene Therapy ◽  
Cancer Gene Therapy ◽  
Cancer Gene
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