Solid-supported lipid bilayers – A versatile tool for the structural and functional characterization of membrane proteins

Methods ◽  
2020 ◽  
Vol 180 ◽  
pp. 56-68 ◽  
Author(s):  
Jakob Andersson ◽  
Pierluigi Bilotto ◽  
Laura L.E. Mears ◽  
Stefan Fossati ◽  
Ulrich Ramach ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Lipid Bilayers ◽  
Functional Characterization ◽  
Supported Lipid Bilayers ◽  
Versatile Tool

scholarly journals Preparation and Characterization of Solid-Supported Lipid Bilayers Formed by Langmuir–Blodgett Deposition: A Tutorial

Langmuir ◽  
2018 ◽  
Vol 34 (51) ◽  
pp. 15622-15639 ◽  
Author(s):  
James Kurniawan ◽  
João Francisco Ventrici de Souza ◽  
Amanda T. Dang ◽  
Gang-yu Liu ◽  
Tonya L. Kuhl
Keyword(s):  
Lipid Bilayers ◽  
Supported Lipid Bilayers ◽  
Langmuir Blodgett

Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains

2021 ◽  
pp. 243-256
Author(s):  
Romina F. Vázquez ◽  
Erasmo Ovalle-García ◽  
Armando Antillón ◽  
Iván Ortega-Blake ◽  
Carlos Muñoz-Garay ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Supported Lipid Bilayers ◽  
Nanoscale Characterization

scholarly journals A cell-free method for expressing and reconstituting membrane proteins enables functional characterization of the plant receptor-like protein kinase FERONIA

2017 ◽  
Vol 292 (14) ◽  
pp. 5932-5942 ◽  
Author(s):  
Benjamin B. Minkoff ◽  
Shin-ichi Makino ◽  
Miyoshi Haruta ◽  
Emily T. Beebe ◽  
Russell L. Wrobel ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Membrane Proteins ◽  
Functional Characterization ◽  
A Cell

The Use of Xenopus laevis Oocytes for the Functional Characterization of Heterologously Expressed Membrane Proteins

2000 ◽  
Vol 10 (1-2) ◽  
pp. 1-12 ◽  
Author(s):  
Carsten A. Wagner ◽  
Björn Friedrich ◽  
Iwan Setiawan ◽  
Florian Lang ◽  
Stefan Bröer
Keyword(s):  
Membrane Proteins ◽  
Xenopus Laevis ◽  
Functional Characterization ◽  
Xenopus Laevis Oocytes

scholarly journals Towards a native environment: structure and function of membrane proteins in lipid bilayers by NMR

2021 ◽  
Author(s):  
Kai Xue ◽  
Kumar Tekwani Movellan ◽  
Xizhou Cecily Zhang ◽  
Eszter E. Najbauer ◽  
Marcel C. Forster ◽  
...  
Keyword(s):  
Solid State ◽  
Membrane Proteins ◽  
Small Molecules ◽  
Lipid Bilayers ◽  
Biological Samples ◽  
Solid State Nmr ◽  
Structure And Function ◽  
Versatile Technique ◽  
And Function

Solid-state NMR (ssNMR) is a versatile technique that can be used for the characterization of various materials, ranging from small molecules to biological samples, including membrane proteins, as reviewed here.

scholarly journals Functional Characterization of Pneumocystis carinii brl1 by Transspecies Complementation Analysis

2007 ◽  
Vol 6 (12) ◽  
pp. 2448-2452 ◽  
Author(s):  
Libera Lo Presti ◽  
Moira Cockell ◽  
Lorenzo Cerutti ◽  
Viesturs Simanis ◽  
Philippe M. Hauser
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Membrane Proteins ◽  
Nuclear Membrane ◽  
Opportunistic Infections ◽  
Functional Characterization ◽  
Pneumocystis Carinii ◽  
Ectopic Expression ◽  
Functional Complementation ◽  
Null Alleles

ABSTRACT Pneumocystis jirovecii is a fungus which causes severe opportunistic infections in immunocompromised humans. The brl1 gene of P. carinii infecting rats was identified and characterized by using bioinformatics in conjunction with functional complementation in Saccharomyces cerevisiae and Schizosaccharomyces pombe. The ectopic expression of this gene rescues null alleles of essential nuclear membrane proteins of the Brr6/Brl1 family in both yeasts.

scholarly journals Characterization of supported lipid bilayers incorporating and phosphoinositol-3,4,5-triphosphate by complementary techniques

2010 ◽  
Vol 5 (4) ◽  
pp. 114-119 ◽  
Author(s):  
Martina K. Baumann ◽  
Esther Amstad ◽  
Alireza Mashaghi ◽  
Marcus Textor ◽  
Erik Reimhult
Keyword(s):  
Lipid Bilayers ◽  
Supported Lipid Bilayers ◽  
Complementary Techniques
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