Live-cell imaging of budding yeast telomerase RNA and TERRA

Methods ◽  
2017 ◽  
Vol 114 ◽  
pp. 46-53 ◽  
Author(s):  
Hadrien Laprade ◽  
Maxime Lalonde ◽  
David Guérit ◽  
Pascal Chartrand
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Budding Yeast ◽  
Live Cell ◽  
Telomerase Rna ◽  
Yeast Telomerase

scholarly journals Live Cell Imaging of Telomerase RNA Dynamics Reveals Cell Cycle-Dependent Clustering of Telomerase at Elongating Telomeres

2011 ◽  
Vol 44 (5) ◽  
pp. 819-827 ◽  
Author(s):  
Franck Gallardo ◽  
Nancy Laterreur ◽  
Emilio Cusanelli ◽  
Faissal Ouenzar ◽  
Emmanuelle Querido ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Telomerase Rna ◽  
Rna Dynamics ◽  
Cycle Dependent

scholarly journals Live Cell Imaging of Mitochondrial Movement along Actin Cables in Budding Yeast

2004 ◽  
Vol 14 (22) ◽  
pp. 1996-2004 ◽  
Author(s):  
Kammy L. Fehrenbacher ◽  
Hyeong-Cheol Yang ◽  
Anna Card Gay ◽  
Thomas M. Huckaba ◽  
Liza A. Pon
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Budding Yeast ◽  
Live Cell ◽  
Mitochondrial Movement ◽  
Actin Cables

scholarly journals Live-Cell Imaging of mRNP–NPC Interactions in Budding Yeast

2019 ◽  
pp. 131-150 ◽  
Author(s):  
Azra Lari ◽  
Farzin Farzam ◽  
Pierre Bensidoun ◽  
Marlene Oeffinger ◽  
Daniel Zenklusen ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Budding Yeast ◽  
Live Cell

scholarly journals High Temporal Resolution 3D Live-Cell Imaging of Budding Yeast Meiosis Defines Discontinuous Actin/Telomere-Mediated Chromosome Motion, Correlated Nuclear Envelope Deformation and Actin Filament Dynamics

2021 ◽  
Vol 9 ◽  
Author(s):  
Tadasu Nozaki ◽  
Frederick Chang ◽  
Beth Weiner ◽  
Nancy Kleckner
Keyword(s):  
Nuclear Envelope ◽  
Temporal Resolution ◽  
Meiotic Prophase ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Budding Yeast ◽  
Three Dimensional ◽  
Live Cell ◽  
High Temporal Resolution ◽  
Homolog Pairing

Chromosome movement is prominent at mid-meiotic prophase and is proposed to enhance the efficiency and/or stringency of homolog pairing and/or to help prevent or resolve topological entanglements. Here, we combine fluorescent repressor operator system (FROS) labeling with three-dimensional (3D) live-cell imaging at high spatio-temporal resolution to define the detailed kinetics of mid-meiotic prophase motion for a single telomere-proximal locus in budding yeast. Telomere motions can be grouped into three general categories: (i) pauses, in which the telomere “jiggles in place”; (ii) rapid, straight/curvilinear motion which reflects Myo2/actin-mediated transport of the monitored telomere; and (iii) slower directional motions, most of which likely reflect indirectly promoted motion of the monitored telomere in coordination with actin-mediated motion of an unmarked telomere. These and other findings highlight the importance of dynamic assembly/disassembly of telomere/LINC/actin ensembles and also suggest important roles for nuclear envelope deformations promoted by actin-mediated telomere/LINC movement. The presented low-SNR (signal-to-noise ratio) imaging methodology provides opportunities for future exploration of homolog pairing and related phenomena.

Sign in / Sign up

Export Citation Format

Share Document