Calorimetric quantification of linked equilibria in cyclodextrin/lipid/detergent mixtures for membrane-protein reconstitution

Methods ◽  
2015 ◽  
Vol 76 ◽  
pp. 183-193 ◽  
Author(s):  
Martin Textor ◽  
Carolyn Vargas ◽  
Sandro Keller
Keyword(s):  
Membrane Protein ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution

scholarly journals Oriented Membrane Protein Reconstitution into Tethered Lipid Membranes for AFM Force Spectroscopy

2016 ◽  
Vol 111 (9) ◽  
pp. 1925-1934 ◽  
Author(s):  
Anna M. Bronder ◽  
Adeline Bieker ◽  
Shantha Elter ◽  
Manuel Etzkorn ◽  
Dieter Häussinger ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Lipid Membranes ◽  
Force Spectroscopy ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution

scholarly journals Self-Assembly of Polymer-Encased Lipid Nanodiscs and Membrane Protein Reconstitution

2019 ◽  
Vol 123 (21) ◽  
pp. 4562-4570 ◽  
Author(s):  
Bikash R. Sahoo ◽  
Takuya Genjo ◽  
Kanhu C. Moharana ◽  
Ayyalusamy Ramamoorthy
Keyword(s):  
Membrane Protein ◽  
Self Assembly ◽  
Membrane Protein Reconstitution ◽  
Lipid Nanodiscs ◽  
Protein Reconstitution

scholarly journals A procedure for membrane-protein reconstitution and the functional reconstitution of the anion transport system of the human-erythrocyte membrane

1980 ◽  
Vol 189 (1) ◽  
pp. 35-44 ◽  
Author(s):  
J M Wolosin
Keyword(s):  
Membrane Protein ◽  
Anion Exchange ◽  
Exchange System ◽  
Protein Preparation ◽  
Protein Properties ◽  
Anion Transport System ◽  
Functional Features ◽  
Basic Features ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution

A short procedure for the isolation of band-3 protein, the protein responsible for anion exchange in erythrocytes, in a reasonable degree of purity was developed. Using this protein preparation and a novel procedure for membrane-protein reconstitution, vesicles displaying the basic features of the anion-exchange system of the erythrocyte were obtained. The reconstitution procedure is based on slow direct removal of Triton X-100 from aqueous lipid/detergent solutions. According to the composition of the reconstitution medium, either small single-walled or large multi-walled vesicles are obtained. The procedure conserves protein properties well, as is revealed by the similarity of the rates of SO4(2-) exchange in erythrocytes and reconstituted vesicles when corrected for the relevant volumes. A number of functional features of the exchange system were studied and compared with those of the native membrane.

scholarly journals Membrane protein reconstitution and crystallization by controlled dilution

FEBS Letters ◽  
2003 ◽  
Vol 555 (1) ◽  
pp. 160-169 ◽  
Author(s):  
H.-W Rémigy ◽  
D Caujolle-Bert ◽  
K Suda ◽  
A Schenk ◽  
M Chami ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution

scholarly journals Synthesis and characterization of tethered lipid assemblies for membrane protein reconstitution (Review)

2017 ◽  
Vol 12 (4) ◽  
pp. 04E301 ◽  
Author(s):  
Rémi Veneziano ◽  
Claire Rossi ◽  
Alexandre Chenal ◽  
Catherine Brenner ◽  
Daniel Ladant ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Synthesis And Characterization ◽  
Lipid Assemblies ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution

Frontal affinity chromatographic analysis of membrane protein reconstitution

1997 ◽  
Vol 4 (4) ◽  
pp. 221-226 ◽  
Author(s):  
Andreas Lundqvist ◽  
Eggert Brekkan ◽  
Christine Lagerquist ◽  
Lars Haneskog ◽  
Per Lundahi
Keyword(s):  
Membrane Protein ◽  
Chromatographic Analysis ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution

Liposome Solubilization and Membrane Protein Reconstitution Using Chaps and Chapso

1997 ◽  
Vol 243 (3) ◽  
pp. 798-804 ◽  
Author(s):  
Josep Cladera ◽  
Jean-Louis Ricaud ◽  
Joaquim Villa Verde ◽  
Mireia DuNach
Keyword(s):  
Membrane Protein ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution ◽  
Liposome Solubilization
Sign in / Sign up

Export Citation Format

Share Document