Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level

Methods ◽  
2013 ◽  
Vol 59 (1) ◽  
pp. 59-70 ◽  
Author(s):  
Karin Norrman ◽  
Anna Strömbeck ◽  
Henrik Semb ◽  
Anders Ståhlberg
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Definitive Endoderm ◽  
Single Cell Level ◽  
Cell Level ◽  
Distinct Gene

scholarly journals Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells

2016 ◽  
Author(s):  
Stefan Semrau ◽  
Johanna Goldmann ◽  
Magali Soumillon ◽  
Tarjei S. Mikkelsen ◽  
Rudolf Jaenisch ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Embryonic Stem ◽  
Lineage Commitment ◽  
Single Cell Level ◽  
Cell Level ◽  
Expression Variability ◽  
Gene Expression Heterogeneity

ABSTRACTGene expression heterogeneity in the pluripotent state of mouse embryonic stem cells (mESCs) has been increasingly well-characterized. In contrast, exit from pluripotency and lineage commitment have not been studied systematically at the single-cell level. Here we measured the gene expression dynamics of retinoic acid driven mESC differentiation using an unbiased single-cell transcriptomics approach. We found that the exit from pluripotency marks the start of a lineage bifurcation as well as a transient phase of susceptibility to lineage specifying signals. Our study revealed several transcriptional signatures of this phase, including a sharp increase of gene expression variability. Importantly, we observed a handover between two classes of transcription factors. The early-expressed class has potential roles in lineage biasing, the late-expressed class in lineage commitment. In summary, we provide a comprehensive analysis of lineage commitment at the single cell level, a potential stepping stone to improved lineage control through timing of differentiation cues.

Single-cell c-myc gene expression in human embryonic stem cells and human teratocarcinoma NTERA cells

2008 ◽  
Vol 2008 (Spring) ◽  
Author(s):  
Gabriela Galiová ◽  
Eva Bártová ◽  
Andrea Harničarová ◽  
Jana Krejčí ◽  
Stanislav Kozubek
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Myc Gene

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression

scholarly journals Single-Cell Transcriptome Analysis as a Promising Tool to Study Pluripotent Stem Cell Reprogramming

2021 ◽  
Vol 22 (11) ◽  
pp. 5988
Author(s):  
Hyun Kyu Kim ◽  
Tae Won Ha ◽  
Man Ryul Lee
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
Cell Fate ◽  
Human Cell ◽  
Transcriptome Analysis ◽  
Single Cell Analysis ◽  
Embryonic Stem ◽  
Single Cell Level ◽  
Cell Analysis ◽  
Cell Level

Cells are the basic units of all organisms and are involved in all vital activities, such as proliferation, differentiation, senescence, and apoptosis. A human body consists of more than 30 trillion cells generated through repeated division and differentiation from a single-cell fertilized egg in a highly organized programmatic fashion. Since the recent formation of the Human Cell Atlas consortium, establishing the Human Cell Atlas at the single-cell level has been an ongoing activity with the goal of understanding the mechanisms underlying diseases and vital cellular activities at the level of the single cell. In particular, transcriptome analysis of embryonic stem cells at the single-cell level is of great importance, as these cells are responsible for determining cell fate. Here, we review single-cell analysis techniques that have been actively used in recent years, introduce the single-cell analysis studies currently in progress in pluripotent stem cells and reprogramming, and forecast future studies.

scholarly journals Conditional Gene Expression in Human Embryonic Stem Cells

Stem Cells ◽  
2007 ◽  
Vol 25 (6) ◽  
pp. 1490-1497 ◽  
Author(s):  
Ludovic Vallier ◽  
Morgan Alexander ◽  
Roger Pedersen
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Conditional Gene Expression
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