A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes

Methods ◽  
2012 ◽  
Vol 57 (1) ◽  
pp. 129-137 ◽  
Author(s):  
Eric Largy ◽  
Florian Hamon ◽  
Marie-Paule Teulade-Fichou
Keyword(s):  
Nucleic Acid ◽  
Fluorescent Probes ◽  
Paramagnetic Particle ◽  
Competition Assay

scholarly journals Schnellnachweis von SARS-CoV-2 mit recombinase polymerase amplification

BIOspektrum ◽  
2020 ◽  
Vol 26 (6) ◽  
pp. 624-627
Author(s):  
Ole Behrmann ◽  
Iris Bachmann ◽  
Frank Hufert ◽  
Gregory Dame
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Dna Polymerase ◽  
Fluorescent Probes ◽  
Reverse Transcription ◽  
Nucleic Acid Detection ◽  
Detection Scheme ◽  
Recombination Proteins ◽  
Target Dna ◽  
Rapid Amplification

Abstract The COVID-19 pandemic highlights the need for fast and simple assays for nucleic acid detection. As an isothermal alternative to RT-qPCR, we outline the development of a detection scheme for SARS-CoV-2 RNA based on reverse transcription recombinase polymerase amplification (RT-RPA) technology. RPA uses recombination proteins in combination with a DNA polymerase for rapid amplification of target DNA at a constant temperature (39–42 °C) within 10 to 20 minutes and can be monitored in real-time with fluorescent probes.

Ribonuclease H-cleavable and recombinase-quenching fluorescent probes for the real-time detection of strand invasion based amplification

2019 ◽  
Vol 11 (43) ◽  
pp. 5568-5576
Author(s):  
Sonja Elf ◽  
Kevin E. Eboigbodin
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Real Time ◽  
Fluorescent Probes ◽  
Nucleic Acid Amplification ◽  
Ribonuclease H ◽  
Chain Reaction ◽  
Amplification Method ◽  
Polymerase Chain ◽  
Strand Invasion

SIBA is an established nucleic acid amplification method that is used as an alternative to polymerase chain reaction (PCR).

scholarly journals Chloro-Substituted Naphthyridine Derivative and Its Conjugate with Thiazole Orange for Highly Selective Fluorescence Sensing of an Orphan Cytosine in the AP Site-Containing Duplexes

2020 ◽  
Vol 10 (12) ◽  
pp. 4133
Author(s):  
Chun-xia Wang ◽  
Yusuke Sato ◽  
Takashi Sugimoto ◽  
Norio Teramae ◽  
Seiichi Nishizawa
Keyword(s):  
Nucleic Acid ◽  
Sequence Analysis ◽  
Fluorescent Probes ◽  
Dna Duplexes ◽  
Thiazole Orange ◽  
Fluorescence Sensing ◽  
Binding Selectivity ◽  
Target Dna ◽  
Fluorescence Signaling ◽  
Ap Site

Fluorescent probes with the binding selectivity to specific structures in DNAs or RNAs have gained much attention as useful tools for the study of nucleic acid functions. Here, chloro-substituted 2-amino-5,7-dimethyl-1,8-naphthyridine (ClNaph) was developed as a strong and highly selective binder for target orphan cytosine opposite an abasic (AP) site in the DNA duplexes. ClNaph was then conjugated with thiazole orange (TO) via an alkyl spacer (ClNaph–TO) to design a light-up probe for the detection of cytosine-related mutations in target DNA. In addition, we found the useful binding and fluorescence signaling of the ClNaph–TO conjugate to target C in AP site-containing DNA/RNA hybrid duplexes with a view toward sequence analysis of microRNAs.

Nucleic Acid Fluorescent Probes for Biological Sensing

2012 ◽  
Vol 66 (11) ◽  
pp. 1249-1261 ◽  
Author(s):  
Xin Su ◽  
Xianjin Xiao ◽  
Chen Zhang ◽  
Meiping Zhao
Keyword(s):  
Nucleic Acid ◽  
Fluorescent Probes ◽  
Biological Sensing

8-Azapurines as isosteric purine fluorescent probes for nucleic acid and enzymatic research

2014 ◽  
Vol 10 (11) ◽  
pp. 2756-2774 ◽  
Author(s):  
Jacek Wierzchowski ◽  
Jan M. Antosiewicz ◽  
David Shugar
Keyword(s):  
Nucleic Acid ◽  
Fluorescent Probes ◽  
Fluorescence Emission ◽  
Fluorescence Probes ◽  
Emission Properties ◽  
Biochemical Systems ◽  
Related Compounds

We review fluorescence emission properties of 8-azapurines and related compounds, and their potential as fluorescence probes in various biochemical systems.

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