High-throughput protein expression screening and purification in Escherichia coli

Methods ◽  
2011 ◽  
Vol 55 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Renaud Vincentelli ◽  
Agnès Cimino ◽  
Arie Geerlof ◽  
Atsushi Kubo ◽  
Yutaka Satou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein Expression ◽  
High Throughput ◽  
Expression Screening

scholarly journals High-throughput recombinant protein expression in Escherichia coli : current status and future perspectives

Open Biology ◽  
2016 ◽  
Vol 6 (8) ◽  
pp. 160196 ◽  
Author(s):  
Baolei Jia ◽  
Che Ok Jeon
Keyword(s):  
Escherichia Coli ◽  
Protein Expression ◽  
High Throughput ◽  
Rational Design ◽  
Genetic Manipulation ◽  
Recombinant Protein Expression ◽  
Current System ◽  
Low Cost ◽  
Current Status ◽  
Post Translational Modification

The ease of genetic manipulation, low cost, rapid growth and number of previous studies have made Escherichia coli one of the most widely used microorganism species for producing recombinant proteins. In this post-genomic era, challenges remain to rapidly express and purify large numbers of proteins for academic and commercial purposes in a high-throughput manner. In this review, we describe several state-of-the-art approaches that are suitable for the cloning, expression and purification, conducted in parallel, of numerous molecules, and we discuss recent progress related to soluble protein expression, mRNA folding, fusion tags, post-translational modification and production of membrane proteins. Moreover, we address the ongoing efforts to overcome various challenges faced in protein expression in E. coli , which could lead to an improvement of the current system from trial and error to a predictable and rational design.

A Human cDNA Library for High-Throughput Protein Expression Screening

Genomics ◽  
2000 ◽  
Vol 65 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Konrad Büssow ◽  
Eckhard Nordhoff ◽  
Christine Lübbert ◽  
Hans Lehrach ◽  
Gerald Walter
Keyword(s):  
Protein Expression ◽  
Cdna Library ◽  
High Throughput ◽  
Expression Screening ◽  
Human Cdna
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