Cave Canalem: How endogenous ion channels may interfere with heterologous expression in Xenopus oocytes

Methods ◽  
2010 ◽  
Vol 51 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Jan Terhag ◽  
Nora A. Cavara ◽  
Michael Hollmann
Keyword(s):  
Ion Channels ◽  
Heterologous Expression ◽  
Xenopus Oocytes

scholarly journals Analysis of signal transduction pathways caused by GABAB receptor subtypes - heterologous expression with Xenopus oocytes

2000 ◽  
Vol 82 ◽  
pp. 136
Author(s):  
Yasuhito Uezono ◽  
Muneshige Kaibara ◽  
Hiroki Yokoo ◽  
Hideyuki Kobayashi ◽  
Toshihiko Yanagita ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Heterologous Expression ◽  
Xenopus Oocytes ◽  
Gabab Receptor ◽  
Signal Transduction Pathways ◽  
Receptor Subtypes

scholarly journals TACAN is an essential component of the mechanosensitive ion channel responsible for pain sensing

2018 ◽  
Author(s):  
L. Beaulieu-Laroche ◽  
M. Christin ◽  
AM Donoghue ◽  
F. Agosti ◽  
N. Yousefpour ◽  
...  
Keyword(s):  
Ion Channels ◽  
Heterologous Expression ◽  
Ion Channel ◽  
Behavioral Responses ◽  
Mechanical Stimuli ◽  
Thermal Pain ◽  
Fundamental Process ◽  
Mechanosensitive Ion Channel ◽  
Pain Stimuli ◽  
Essential Subunit

SummaryMechanotransduction, the conversion of mechanical stimuli into electrical signals, is a fundamental process underlying several physiological functions such as touch and pain sensing, hearing and proprioception. This process is carried out by specialized mechanosensitive ion channels whose identities have been discovered for most functions except pain sensing. Here we report the identification of TACAN (Tmem120A), an essential subunit of the mechanosensitive ion channel responsible for sensing mechanical pain. TACAN is expressed in a subset of nociceptors, and its heterologous expression increases mechanically-evoked currents in cell lines. Purification and reconstitution of TACAN in synthetic lipids generates a functional ion channel. Finally, knocking down TACAN decreases the mechanosensitivity of nociceptors and reduces behavioral responses to mechanical but not to thermal pain stimuli, without affecting the sensitivity to touch stimuli. We propose that TACAN is a pore-forming subunit of the mechanosensitive ion channel responsible for sensing mechanical pain.

Heterologous Expression of Ion Channels

Neurogenetics ◽  
2003 ◽  
pp. 285-294
Author(s):  
Andrew R. Tapper ◽  
Alfred L. George
Keyword(s):  
Ion Channels ◽  
Heterologous Expression
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