Hypothesis testing via integrated computer modeling and digital fluorescence microscopy

Methods ◽  
2007 ◽  
Vol 41 (2) ◽  
pp. 232-237 ◽  
Author(s):  
Melissa K. Gardner ◽  
David J. Odde ◽  
Kerry Bloom
Keyword(s):  
Fluorescence Microscopy ◽  
Hypothesis Testing ◽  
Computer Modeling ◽  
Digital Fluorescence Microscopy

Measuring diffusion coefficients of labelled particles on cell surfaces by digital fluorescence microscopy

1990 ◽  
Vol 18 (5) ◽  
pp. 938-938 ◽  
Author(s):  
IAN E. G. MORRISON ◽  
CATHERINE M. ANDERSON ◽  
GEORGE N. GEORGIOU ◽  
RICHARD J. CHERRY
Keyword(s):  
Fluorescence Microscopy ◽  
Diffusion Coefficients ◽  
Cell Surfaces ◽  
Digital Fluorescence Microscopy

scholarly journals Automated high-throughput digital fluorescence microscopy for TB diagnosis

2020 ◽  
Vol 24 (10) ◽  
pp. 1103-1105
Author(s):  
D. Chesov ◽  
V. Lesanu ◽  
N. Ciobanu ◽  
A. Codreanu ◽  
V. Crudu ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
High Throughput ◽  
Digital Fluorescence Microscopy

Visualization of replication sites in unfixed human cells

1993 ◽  
Vol 105 (2) ◽  
pp. 541-550 ◽  
Author(s):  
A.B. Hassan ◽  
P.R. Cook
Keyword(s):  
Dna Replication ◽  
Fluorescence Microscopy ◽  
Hela Cells ◽  
Human Cells ◽  
Underlying Structure ◽  
Fixation Methods ◽  
Replication Sites ◽  
Digital Fluorescence Microscopy ◽  
Replication Activity

Sites of DNA replication in nuclei are focally concentrated, suggesting that an underlying structure organizes the activity of many polymerases. As fixation could induce aggregation into foci, we examined the distribution of replication sites in unfixed nuclei. HeLa cells were encapsulated in agarose microbeads, permeabilized in a ‘physiological’ buffer, their DNA polymerizing activity characterized, and replication sites directly labelled by incubation with fluorochrome-dUTP conjugates. Using conventional and digital fluorescence microscopy, 80–250 foci were seen in these unfixed cells. These foci are unlikely to be formed by the aggregation of separate polymerases as most replication activity found in vivo is retained throughout these procedures. Although commonly used fixation methods collapsed or dispersed their periphery, the central core was very stable. Foci remained when approximately 90% chromatin was removed, suggesting they were attached to an underlying structure.

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