Popliteal lymph node (PLN) assay to study adjuvant effects on respiratory allergy

Methods ◽  
2007 ◽  
Vol 41 (1) ◽  
pp. 72-79 ◽  
Author(s):  
Martinus Løvik ◽  
Torunn Alberg ◽  
Unni Cecilie Nygaard ◽  
Mari Samuelsen ◽  
Else-Carin Groeng ◽  
...  
Keyword(s):  
Lymph Node ◽  
Respiratory Allergy ◽  
Popliteal Lymph Node ◽  
Adjuvant Effects ◽  
Pln Assay

Comparison of Popliteal Lymph Node Responses in Various Strains of Rats

1994 ◽  
Vol 13 (7) ◽  
pp. 455-460 ◽  
Author(s):  
C. Patriarca ◽  
F. Verdier ◽  
J.P. Brouland ◽  
T. Vial ◽  
J. Descotes
Keyword(s):  
Lymph Node ◽  
Side Effects ◽  
Popliteal Lymph Node ◽  
Brown Norway ◽  
Sprague Dawley ◽  
Drug Induced ◽  
Immune Reactions ◽  
Interstrain Differences ◽  
Pln Assay

Outbred (namely Wistar and Sprague-Dawley) and inbred (Wistar-Furth, Lewis, Fisher 344 and Brown-Norway) strains of rats were screened for their responses to reference compounds in the popliteal lymph node (PLN) assay. Streptozotocin and diphenylhydantoin gave positive responses as evidenced by increased weight and cellularity indices in all strains used whereas procainamide, isoniazid and barbital consistently gave negative responses. Although these findings overall are in agreement with previous investigations involving these compounds, the lack of marked interstrain differences in PLN responses argues against a strong immunogenetically controlled mechanism as could be assumed in presumably auto-immune reactions. The question is raised whether drug-induced side-effects predicted by the PLN assay are basically non-autoimmune as suggested by clinical and immunological findings in man.

In vivo lymphoproliferation in the popliteal lymph node (PLN) assay can be inhibited by leflunomide's active metabolite A77 1726

1999 ◽  
Vol 48 (0) ◽  
pp. 111-112
Author(s):  
H. U. Schorlemmer ◽  
R. Kurrle ◽  
R. Schleyerbach ◽  
R. R. Bartlett
Keyword(s):  
Lymph Node ◽  
Active Metabolite ◽  
Popliteal Lymph Node ◽  
Pln Assay

Positive responses to imipramine in the popliteal lymph node assay are due to primary irritation

2001 ◽  
Vol 20 (11) ◽  
pp. 591-595 ◽  
Author(s):  
G Choquet-Kastylevsky ◽  
R Tedone ◽  
J Descotes
Keyword(s):  
Lymph Node ◽  
False Positive ◽  
Positive Response ◽  
Popliteal Lymph Node ◽  
Systemic Autoimmunity ◽  
Popliteal Lymph Node Assay ◽  
Primary Irritation ◽  
Pln Assay ◽  
Rule Out ◽  
Footpad Injection

The popliteal lymph node (PLN) assay has long been proposed as a tool to detect immunotoxicants with the potential to induce systemic autoimmunity. A major problem hampering the further validation of this assay is the need to rule out irritants that cause false-positive PLN responses. The anti-depressant, imipramine, has not been reported to induce systemic autoimmune reactions in treated patients, but has been repeatedly found positive in the PLN assay, suggesting that this is a false-positive response. To test this hypothesis, the effects of imipramine were compared to those of 50% ethanol in C57Bl/6 mice. Footpad edema was evidenced in the few days after injection of both ethanol and imipramine. T-cell depletion using monoclonal antibodies against either CD4+ or CD8+T-lymphocytes prior to the PLN assay did not influence the responses to either ethanol or imipramine. Cytokine (TNF, IL-1, IL-1, IL-2R, IL-6, IL-12 and IFN-) fingerprinting of the PLNs after injection of ethanol and imipramine evidenced the same pattern of responses. These results indicate a closely similar pattern of responses following the footpad injection of either imipramine or ethanol. The conclusion can be drawn that imipramine induces positive responses in the PLN assay via primary (nonspecific) irritation.

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