Construction and expression of intracellular anti-ATF-1 single chain Fv fragment: a modality to inhibit melanoma tumor growth and metastasis

Methods ◽  
2004 ◽  
Vol 34 (2) ◽  
pp. 233-239 ◽  
Author(s):  
C TELLEZ
Keyword(s):  
Tumor Growth ◽  
Single Chain ◽  
Melanoma Tumor ◽  
Single Chain Fv ◽  
Tumor Growth And Metastasis ◽  
Single Chain Fv Fragment

scholarly journals Inhibition of tumor growth and metastasis of human melanoma by intracellular anti-ATF-1 single chain Fv fragment

Oncogene ◽  
2000 ◽  
Vol 19 (22) ◽  
pp. 2721-2730 ◽  
Author(s):  
Didier Jean ◽  
Carmen Tellez ◽  
Suyun Huang ◽  
Darren W Davis ◽  
Christiane J Bruns ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Human Melanoma ◽  
Single Chain ◽  
Single Chain Fv ◽  
Inhibition Of Tumor Growth ◽  
Tumor Growth And Metastasis ◽  
Single Chain Fv Fragment

Anti-Human Epidermal Growth Factor Receptor 2 Single-Chain Fv Fragment-Decorated DM1 Nanoparticles for Specific Targeting of Human Epidermal Growth Factor Receptor 2-Positive Breast Tumor Cells

2021 ◽  
Vol 17 (3) ◽  
pp. 447-455
Author(s):  
Ling Ni ◽  
You-Xin Li
Keyword(s):  
Breast Cancer ◽  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Factor Receptor ◽  
Single Chain ◽  
Human Epidermal Growth Factor ◽  
Single Chain Fv ◽  
Epidermal Growth ◽  
Single Chain Fv Fragment

Purpose: Although monoclonal antibodies are used to decorate nanoparticles to target specific cells, penetration of tumor tissues by monoclonal antibodies is limited by their large size. Therefore, we prepared DM1 nanoparticles decorated with the small anti-HER2 single-chain Fv fragment (scFvHER2) of trastuzumab (TMAB) for targeting to human epidermal growth factor receptor 2 (HER2) overexpressing in breast cancer effectively. Methods: ScFvHER2 fragment was coupled with DM1 nanoparticles (NPs) via covalent thiol-maleimide linkages. Their physicochemical properties, uptake by cells, and toxicity to tumor cells were investigated. Their vivo biodistribution was assessed employing liquid chromatographytandem mass spectrometry, while their antitumor activity was investigated in nude mice burdened with BT-474 tumor. Results: Viability of BT-474 cells incubated with scFvHER2-DM1-Nanoparticles (scFv-DM1-NPs) was significantly lower than that of BT-474 cell treated with TMAB-DM1-Nanoparticles (TMAB-DM1-NPs) (P < 0 05). Uptake by cells of scFvDM1-NPs was significantly higher than TMAB-DM1-NPs (P < 0 01). Accumulation of scFv-DM1-NPs in tumor tissue was notably higher than TMAB-DM1-NPs (P < 0 05). scFv-DM1-NPs exhibited improved antitumor effects compared to TMABDM1-NPs (P < 0 05), showing a tumor inhibition rate of more than 70%. Conclusions: ScFvHER2 fragment could serve as a more effective targeting ligand than TMAB, and scFv-DM1-NPs could be developed as a possible drug delivery system to target HER2-positive breast cancer.

scholarly journals Systemic Platelet-activating Factor Receptor Activation Augments Experimental Lung Tumor Growth and Metastasis

2014 ◽  
Vol 7 ◽  
pp. CGM.S14501 ◽  
Author(s):  
Patrick C. Hackler ◽  
Sarah Reuss ◽  
Raymond L. Konger ◽  
Jeffrey B. Travers ◽  
Ravi P. Sahu
Keyword(s):  
Lung Cancer ◽  
Tumor Growth ◽  
Cancer Progression ◽  
Lung Tumor ◽  
Platelet Activating Factor ◽  
Receptor Activation ◽  
Dependent Manner ◽  
Melanoma Tumor ◽  
Tumor Growth And Metastasis ◽  
The Impact

Pro-oxidative stressors including cigarette smoke (CS) generate novel lipids with platelet-activated factor-receptor (PAF-R) agonistic activity mediate systemic immunosuppression, one of the most recognized events in promoting carcinogenesis. Our previous studies have established that these oxidized-PAF-R-agonists augment murine B16F10 melanoma tumor growth in a PAF-R-dependent manner because of its effects on host immunity. As CS generates PAF-R agonists, the current studies sought to determine the impact of PAF-R agonists on lung cancer growth and metastasis. Using the murine Lewis Lung Carcinoma (LLC1) model, we demonstrate that treatment of C57BL/6 mice with a PAF-R agonist augments tumor growth and lung metastasis in a PAF-R-dependent manner as these findings were not seen in PAF-R-deficient mice. Importantly, this effect was because of host rather than tumor cells PAF-R dependent as LLC1 cells do not express functional PAF-R. These findings indicate that experimental lung cancer progression can be modulated by the PAF system.

scholarly journals Genetic Ablation of Cav1 Differentially Affects Melanoma Tumor Growth and Metastasis in Mice: Role of Cav1 in Shh Heterotypic Signaling and Transendothelial Migration

2012 ◽  
Vol 72 (9) ◽  
pp. 2262-2274 ◽  
Author(s):  
Franco Capozza ◽  
Casey Trimmer ◽  
Remedios Castello-Cros ◽  
Sanjay Katiyar ◽  
Diana Whitaker-Menezes ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Transendothelial Migration ◽  
Melanoma Tumor ◽  
Genetic Ablation ◽  
Tumor Growth And Metastasis

A bivalent single-chain Fv fragment against CD47 induces apoptosis for leukemic cells

2004 ◽  
Vol 315 (4) ◽  
pp. 912-918 ◽  
Author(s):  
Yasufumi Kikuchi ◽  
Shinsuke Uno ◽  
Yasushi Yoshimura ◽  
Koji Otabe ◽  
Shin-ichiro Iida ◽  
...  
Keyword(s):  
Leukemic Cells ◽  
Single Chain ◽  
Single Chain Fv ◽  
Single Chain Fv Fragment
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