scholarly journals Analysis of polysomal mRNA populations of mouse oocytes and zygotes: Dynamic changes in maternal mRNA utilization and function

2006 ◽  
Vol 298 (1) ◽  
pp. 155-166 ◽  
Author(s):  
Santhi Potireddy ◽  
Rita Vassena ◽  
Bela G. Patel ◽  
Keith E. Latham
Keyword(s):  
Dynamic Changes ◽  
Mouse Oocytes ◽  
Maternal Mrna ◽  
Polysomal Mrna ◽  
And Function

Synthesis and phosphorylation of uvomorulin during mouse early development

Development ◽  
1992 ◽  
Vol 115 (1) ◽  
pp. 313-318 ◽  
Author(s):  
M. Sefton ◽  
M.H. Johnson ◽  
L. Clayton
Keyword(s):  
Cell Adhesion ◽  
Early Development ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Blastocyst Stage ◽  
Embryonic Stage ◽  
Cell Stage ◽  
Mouse Oocytes ◽  
Maternal Mrna ◽  
Embryonic Genome

The cell adhesion molecule, uvomorulin, is synthesised in both the 135 × 10(3) M(r) precursor and 120 × 10(3) M(r) mature forms on maternal mRNA templates in unfertilized and newly fertilized mouse oocytes. Synthesis on maternal message ceases during the 2-cell stage to resume later on mRNA encoded presumptively by the embryonic genome. Uvomorulin is detectable by immunoblotting at all stages upto the blastocyst stage, but shows variations in its total amount and processing with embryonic stage. Whilst only trace levels of phosphorylated uvomorulin are detectable in early and late 4-cell embryos, uvomorulin in 8-cell embryos is phosphorylated.

scholarly journals Characterization of the dynamic changes in left ventricular morphology and function induced by exercise training and detraining

2019 ◽  
Vol 277 ◽  
pp. 178-185 ◽  
Author(s):  
Attila Oláh ◽  
Attila Kovács ◽  
Árpád Lux ◽  
Márton Tokodi ◽  
Szilveszter Braun ◽  
...  
Keyword(s):  
Exercise Training ◽  
Left Ventricular ◽  
Dynamic Changes ◽  
And Function ◽  
Left Ventricular Morphology ◽  
Training And Detraining

In vitro fertilisation of mouse oocytes reconstructed by transfer of metaphase II chromosomes results in live births

Zygote ◽  
2001 ◽  
Vol 9 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Min-Kang Wang ◽  
Da-Yuan Chen ◽  
Ji-Long Lui ◽  
Guang-Peng Li ◽  
Qing-Yuan Sun
Keyword(s):  
Nuclear Transfer ◽  
Normal Structure ◽  
Human Reproduction ◽  
In Vitro Fertilisation ◽  
Kunming Mouse ◽  
Mouse Oocytes ◽  
Assisted Human Reproduction ◽  
And Function ◽  
Apparatus Transfer

The interaction between nucleus and cytoplasm can be explored through nuclear transfer. We describe here another tool to investigate this interaction: MII meiotic apparatus transfer (MAT) between mouse oocytes. In this study, the MII oocyte meiotic apparatus or spindle from C57BL/6 mice, a black strain, was transferred into an enucleated metaphase oocyte from Kunming mouse, a white strain. The results showed that the enucleation rate by treating oocytes with 3% sucrose was 100%, but the electrofusion efficiency was very low, with only 17.6% of reconstructed karyoplast-recipient cytoplasm pairs fused. When the fused oocytes were exposed to spermatozoa from C57BL/6 mice, 9 of 11 (82%) were fertilised. Eight reconstructed embryos at 1- to 4-cell stages were transferred into the oviducts of two synchronously pregnant Kunming strain fosters and one delivered two normal C57BL/6 offspring. This study indicates that MII meiotic apparatus or spindle sustains normal structure and function after micromanipulation and electrofusion. MAT provides a model for further research on the application of this technique to assisted human reproduction.

scholarly journals Ran GTP and importin β regulate meiosis I spindle assembly and function in mouse oocytes

2019 ◽  
Vol 39 (1) ◽  
Author(s):  
David Drutovic ◽  
Xing Duan ◽  
Rong Li ◽  
Petr Kalab ◽  
Petr Solc
Keyword(s):  
Spindle Assembly ◽  
Mouse Oocytes ◽  
Ran Gtp ◽  
And Function ◽  
Meiosis I

scholarly journals Dynamic changes in the association between maternal mRNAs and endoplasmic reticulum during ascidian early embryogenesis

2021 ◽  
Author(s):  
Toshiyuki Goto ◽  
Shuhei Torii ◽  
Aoi Kondo ◽  
Junji Kawakami ◽  
Haruka Yagi ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Translational Regulation ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Early Embryogenesis ◽  
Axis Formation ◽  
Second Phase ◽  
Dynamic Changes ◽  
Maternal Mrna ◽  
Maternal Mrnas

AbstractAxis formation is one of the most important events occurring at the beginning of animal development. In the ascidian egg, the antero-posterior axis is established at this time owing to a dynamic cytoplasmic movement called cytoplasmic and cortical reorganisation. During this movement, mitochondria, endoplasmic reticulum (ER), and maternal mRNAs (postplasmic/PEM RNAs) are translocated to the future posterior side. Although accumulating evidence indicates the crucial roles played by the asymmetrical localisation of these organelles and the translational regulation of postplasmic/PEM RNAs, the organisation of ER has not been described in sufficient detail to date owing to technical difficulties. In this study, we developed three different multiple staining protocols for visualising the ER in combination with mitochondria, microtubules, or mRNAs in whole-mount specimens. We defined the internally expanded “dense ER” using these protocols and described cisterna-like structures of the dense ER using focused ion beam-scanning electron microscopy. Most importantly, we described the dynamic changes in the colocalisation of postplasmic/PEM mRNAs and dense ER; for example, macho-1 mRNA was detached and excluded from the dense ER during the second phase of ooplasmic movements. These detailed descriptions of the association between maternal mRNA and ER can provide clues for understanding the translational regulation mechanisms underlying axis determination during ascidian early embryogenesis.

scholarly journals Re-polyadenylation occurs predominantly on maternal mRNA degradation intermediates during mammalian oocyte-to-embryo transition

2021 ◽  
Author(s):  
Yusheng Liu ◽  
Yiwei Zhang ◽  
Hu Nie ◽  
Zhonghua Liu ◽  
Jiaqiang Wang ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Mrna Degradation ◽  
Mammalian Oocyte ◽  
Maternal Mrna ◽  
Early Embryos ◽  
Specific Regulation ◽  
And Function ◽  
Post Transcriptional Regulation ◽  
Degradation Intermediates ◽  
Polyadenylated Mrna

The nascent mRNA transcribed in the nucleus is cleaved and polyadenylated before it is transported to the cytoplasm for translation. Polyadenylation can also occur in the cytoplasm for post-transcriptional regulation, especially in neurons, oocytes and early embryos. Recently, we revealed transcriptome-wide maternal mRNA cytoplasmic re-polyadenylation during the mammalian oocyte-to-embryo transition (OET). However, the mechanism of re-polyadenylation during mammalian OET, including the sites to be re-polyadenylated and the enzymes involved, is still poorly understood. Here, by analyzing the PAIso-seq1 and PAIso-seq2 poly(A) inclusive transcriptome data during OET in mice, rats, pigs, and humans, we reveal conserved re-polyadenylation of mRNA degradation intermediates. These re-polyadenylated mRNA degradation intermediates account for over half of the polyadenylated mRNA during OET in all four species. We find that mRNA degradation intermediates for re-polyadenylation are generated through Btg4-mediated deadenylation in both mouse and human. Interestingly, the poly(A) tails on the re-polyadenylated mRNA degradation intermediates are of different lengths and contain different levels of non-A residues compared to regular polyadenylation sites, suggesting specific regulation and function of these poly(A) tails in mammalian OET. Together, our findings reveal the maternal mRNA degradation intermediates as substrates for conserved cytoplasmic dominant re-polyadenylation during mammalian OET, and uncover the mechanism of production of these mRNA degradation intermediates. These findings provide new insights into mRNA post-transcriptional regulation, and a new direction for the study of mammalian OET.

Vulnerability of islets in the immediate posttransplantation period. Dynamic changes in structure and function

Diabetes ◽  
1996 ◽  
Vol 45 (9) ◽  
pp. 1161-1167 ◽  
Author(s):  
A. M. Davalli ◽  
L. Scaglia ◽  
D. H. Zangen ◽  
J. Hollister ◽  
S. Bonner-Weir ◽  
...  
Keyword(s):  
Structure And Function ◽  
Dynamic Changes ◽  
And Function

scholarly journals Dynamic changes in display architecture and function across environments revealed by a systems approach to animal communication*

Evolution ◽  
2018 ◽  
Vol 72 (5) ◽  
pp. 1134-1145 ◽  
Author(s):  
Malcolm F. Rosenthal ◽  
Matthew R. Wilkins ◽  
Daizaburo Shizuka ◽  
Eileen A. Hebets
Keyword(s):  
Systems Approach ◽  
Animal Communication ◽  
Dynamic Changes ◽  
And Function

scholarly journals Dynamic changes in microbial community structure and function in phenol-degrading microcosms inoculated with cells from a contaminated aquifer

2010 ◽  
Vol 71 (2) ◽  
pp. 247-259 ◽  
Author(s):  
David R. Elliott ◽  
Julie D. Scholes ◽  
Steven F. Thornton ◽  
Athanasios Rizoulis ◽  
Steven A. Banwart ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Structure And Function ◽  
Dynamic Changes ◽  
Contaminated Aquifer ◽  
And Function

Dynamic changes of pancreatic structure and function in rats treated chronically with nicotine

1990 ◽  
Vol 104 (3) ◽  
pp. 457-465 ◽  
Author(s):  
Paul P. Lau ◽  
Michael A. Dubick ◽  
Gloria S.M. Yu ◽  
Paul R. Morrill ◽  
Michael C. Geokas
Keyword(s):  
Structure And Function ◽  
Dynamic Changes ◽  
And Function
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