scholarly journals An optimized protocol for coupling oxygen consumption rates with β-oxidation in isolated mitochondria from mouse soleus

2021 ◽  
Vol 2 (3) ◽  
pp. 100735
Author(s):  
Cristina Sánchez-González ◽  
Laura Formentini
Keyword(s):  
Oxygen Consumption ◽  
Isolated Mitochondria ◽  
Optimized Protocol ◽  
Consumption Rates

scholarly journals REDUCED CITRATE SYNTHASE ACTIVITY EFFECT ON OXYGEN CONSUMPTION RATES IN ISOLATED MITOCHONDRIA FROM MICE LIVER AND MUSCLES

2016 ◽  
Vol 2 (101) ◽  
pp. 26-30
Author(s):  
Andrej Fokin ◽  
Rasa Žūkienė ◽  
Aivaras Ratkevičius
Keyword(s):  
Oxygen Consumption ◽  
Mitochondrial Respiration ◽  
Skeletal Muscles ◽  
Citrate Synthase ◽  
Oxygen Electrode ◽  
Isolated Mitochondria ◽  
Activity Effect ◽  
Consumption Rates ◽  
Centrifugation Method ◽  
Mice Liver

Background. Liver and skeletal muscles play the major role in metabolism. Mitochondria are of particular importance in functioning of these organs. We tested the hypothesis that reduced citrate synthase (CS) activity could induce improved fatty substrate and carbohydrate oxidation in mitochondria extracted from liver and hind limb muscles of mice. Methods. Eight mice each of 12-week-old control C57B6/J (B6) and congenic B6.A-(rs3676616-D10Utsw1)/ Kjn (B6.A) mice were studied. The mitochondria were isolated by differential centrifugation method followed by assessment of mitochondrial respiration and citrate synthase (CS) activity. Mitochondrial respiration was measured as oxygen consumption with Clark-type oxygen electrode by using polarography system. CS enzyme activity was measured spectrophotometrically. Results. The activity of CS was by ~32% lower for mitochondria for B6.A compared to B6 mice (603.9 ± 135.6 U/g and 894.2 ± 193.2 U/g, respectively). Mitochondrial respiration did not differ significantly between the strains. Conclusions. 30% reduction in citrate synthase activity does not impair mitochondrial respiration.

Effects of 5-bromo-2′-deoxyuridine on Friend erythroleukaemia cells. II. Oxidative metabolism and enzyme content of whole cells and isolated mitochondria

1981 ◽  
Vol 52 (1) ◽  
pp. 37-54
Author(s):  
R.J. Walter
Keyword(s):  
Oxygen Consumption ◽  
Respiratory Control ◽  
High Energy ◽  
Inhibitory Effect ◽  
Mitochondrial Enzymes ◽  
Morphological Alterations ◽  
Isolated Mitochondria ◽  
Outer Compartment ◽  
Consumption Rates ◽  
Electrode Chamber

Untreated or bromodeoxyuridine (BrdUrd)-treated Friend erythroleukaemia (EL) cells from 15- and 72-h-cultures were harvested and the mitochondria were isolated by homogenization and differential centrifugation. Aliquots of the original cell suspensions or the final mitochondrial suspensions were either fixed for electron microscopy, assayed for enzyme activities, or introduced into a 1-ml Clarke oxygen electrode chamber. Whole BrdUrd-treated cells exhibited notable morphological alterations (see accompanying paper) but no effect was observed on whole-cell respiration. Morphologically, isolated mitochondria exhibited a highly condensed matrix and a greatly expanded outer compartment. Functionally, these mitochondria oxidized a variety of substrates at high state-3 (ADP-stimulated) rates (60-210 ng atoms O/min per mg protein) and displayed adequate respiratory control and ADP/O ratios. In the mitochondria isolated from BrdUrd-treated EL cells (both 15- and 72-h cultures), the state-3 oxygen consumption rates, respiratory control ratios, and ADP/O ratios generally decreased compared to their matched controls. These functional deficiencies coincided with in situ increases in the volume of the mitochondrial compartment and conspicuous (30-100%) increases in the specific and total activities of several mitochondrial enzymes. In addition, the mitochondria isolated from the 72-h group (treated and untreated) displayed immediate alterations when BrdUrd was added to the electrode chamber. Oxygen consumption rates dropped, respiratory control ratios changed moderately, and ADP/O ratios increased. BrdUrd may act both as an inhibitor of oxidative phosphorylation and perhaps as a sink for some of the high energy phosphate that is being generated. Thus, BrdUrd may exert its inhibitory effect on cell differentiation by interfering with mitochondrial function.

scholarly journals Oxygen consumption rates in subseafloor basaltic crust derived from a reaction transport model

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Beth N. Orcutt ◽  
C. Geoffrey Wheat ◽  
Olivier Rouxel ◽  
Samuel Hulme ◽  
Katrina J. Edwards ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Transport Model ◽  
Consumption Rates ◽  
Reaction Transport ◽  
Basaltic Crust

The Relation of Oxygen Consumption to Body Size and to Temperature in the Larvae of Chironomus Riparius Meigen

1958 ◽  
Vol 35 (2) ◽  
pp. 383-395
Author(s):  
R. W. EDWARDS
Keyword(s):  
Oxygen Consumption ◽  
Body Size ◽  
Dry Matter ◽  
Larval Instar ◽  
Weight Ratio ◽  
Dry Weight ◽  
Chironomus Riparius ◽  
Consumption Rates ◽  
Rate Of Oxygen Consumption ◽  
Wet Weight

1. The oxygen consumption rates of 3rd- and 4th-instar larvae of Chironomus riparius have been measured at 10 and 20° C. using a constant-volume respirometer. 2. The oxygen consumption is approximately proportional to the 0.7 power of the dry weight: it is not proportional to the estimated surface area. 3. This relationship between oxygen consumption and dry weight is the same at 10 and at 20° C.. 4. The rate of oxygen consumption at 20° C. is greater than at 10° C. by a factor of 2.6. 5. During growth the percentage of dry matter of 4th-instar larvae increases from 10 to 16 and the specific gravity from 1.030 to 1.043. 6. The change in the dry weight/wet weight ratio during the 4 larval instar supports the theory of heterauxesis. 7. At 20° C., ‘summer’ larvae respire faster than ‘winter’ larvae.

scholarly journals Mitochondrial Fragmentation in Skeletal Muscle Derived Cells from an Old Male Donor May Relate to Decreased Oxygen Consumption Rates

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
José Adan Arevalo ◽  
Marvin L. Miller ◽  
José Pablo Vazquez-Medina ◽  
George A. Brooks
Keyword(s):  
Skeletal Muscle ◽  
Oxygen Consumption ◽  
Mitochondrial Fragmentation ◽  
Male Donor ◽  
Consumption Rates

scholarly journals Direct measurement of oxygen consumption rates from attached and unattached cells in a reversibly sealed, diffusionally isolated sample chamber

2010 ◽  
Vol 01 (05) ◽  
pp. 398-408 ◽  
Author(s):  
Timothy J. Strovas ◽  
Sarah C. McQuaide ◽  
Judy B. Anderson ◽  
Vivek Nandakumar ◽  
Marina G. Kalyuzhnaya ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Direct Measurement ◽  
Consumption Rates ◽  
Sample Chamber
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