Molecular characterization of Leishmania spp. isolated from Brazilian stray dogs from an endemic area for canine visceral leishmaniasis

2017 ◽  
Vol 7 ◽  
pp. 9-13 ◽  
Author(s):  
Carla Janeiro Coiro ◽  
Laiza Gabriela Gavioli Coelho ◽  
Rodrigo Costa da Silva ◽  
Helio Langoni
Keyword(s):  
Visceral Leishmaniasis ◽  
Molecular Characterization ◽  
Endemic Area ◽  
Canine Visceral Leishmaniasis ◽  
Stray Dogs ◽  
Leishmania Spp

scholarly journals Seroprevalence and molecular characterization of Leishmania in dogs from an endemic area of zoonotic visceral leishmaniasis in Brazil

2017 ◽  
Vol 5 (1) ◽  
pp. 70-74 ◽  
Author(s):  
Josiane V. Lopes ◽  
Érika M. Michalsky ◽  
Fabiana de O. Lara Silva ◽  
Ana Cristina V.M.R. Lima ◽  
Daniel M. de Avelar ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Molecular Characterization ◽  
Endemic Area ◽  
Zoonotic Visceral Leishmaniasis

scholarly journals Molecular characterization of Leishmania infantum in domestic cats in a region of Brazil endemic for human and canine visceral leishmaniasis

Acta Tropica ◽  
2017 ◽  
Vol 166 ◽  
pp. 121-125 ◽  
Author(s):  
Isabel Parizotto Metzdorf ◽  
Manoel Sebastião da Costa Lima ◽  
Maria de Fatima Cepa Matos ◽  
Antonio Francisco de Souza Filho ◽  
Rosianne A. de Souza Tsujisaki ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Molecular Characterization ◽  
Leishmania Infantum ◽  
Canine Visceral Leishmaniasis ◽  
Domestic Cats

scholarly journals Can vaccines against canine visceral leishmaniasis interfere with the serological diagnostics recommended by the Brazilian Ministry of Health?

2017 ◽  
Vol 47 (4) ◽  
Author(s):  
Monique Paiva de Campos ◽  
Paula Mello De Luca ◽  
Alinne Rangel dos Santos Renzetti ◽  
Sara Maria Marques de Souza ◽  
Artur Augusto Velho Mendes Júnior ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Enzyme Immunoassay ◽  
Clinical Evaluation ◽  
Laboratory Tests ◽  
Endemic Area ◽  
Canine Visceral Leishmaniasis ◽  
Ministry Of Health ◽  
Evaluation Time ◽  
One Year ◽  
Serological Diagnostics

ABSTRACT: The objective of the current research was to assess seroconversion in dogs immunized with Leishmune® and Leish Tec® vaccines using rapid chromatographic immunoassay DPP® (Dual Path Platform) (DPP CVL) and enzyme immunoassay (EIE) up to one year after the vaccination protocol. The study sample comprised 28 dogs divided into two groups, each group immunized with an anti-CVL vaccine and clinically monitored for one year through clinical evaluation and laboratory tests. 22 (78.5%) dog were monitored. During the evaluation time (T1-30 days, T2-6 months, and T3-1 year after vaccination) the results for all dogs were negative for CVL, except for one animal vaccinated with Leish tec® that seroconverted in the DPP CVL test at T2. Subsequent examinations of this dog were negative. Our results showed that in a non-endemic area, even at different evaluation times, dogs vaccinated against CVL with Leishmune® or Leish tec® did not seroconvert in the serological protocol used by the Brazilian Ministry of Health (DPP/EIE).

scholarly journals Importance of Lutzomyia longipalpis in the dynamics of transmission of canine visceral leishmaniasis in the endemic area of Porteirinha Municipality, Minas Gerais, Brazil

2005 ◽  
Vol 131 (3-4) ◽  
pp. 213-220 ◽  
Author(s):  
João C. França-Silva ◽  
Ricardo A. Barata ◽  
Roberto T. da Costa ◽  
Érika Michalsky Monteiro ◽  
George L. L. Machado-Coelho ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Endemic Area ◽  
Minas Gerais ◽  
Lutzomyia Longipalpis ◽  
Canine Visceral Leishmaniasis

scholarly journals Isolation and molecular characterization of Leishmania infantum in urine from patients with visceral leishmaniasis in Brazil

Acta Tropica ◽  
2018 ◽  
Vol 178 ◽  
pp. 248-251 ◽  
Author(s):  
Manoel Sebastião da Costa Lima ◽  
Andressa Cristina Lopes Hartkopf ◽  
Rosianne A. de Souza Tsujisaki ◽  
Elisa Teruya Oshiro ◽  
Julie Teresa Shapiro ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Molecular Characterization ◽  
Leishmania Infantum

scholarly journals Molecular Analysis of Aquaglyceroporin 1 Gene in Non-Healing Clinical Iso¬lates Obtained from Patients with Cutaneous Leishmaniasis from Central of Iran

2019 ◽  
Author(s):  
Yasaman Alijani ◽  
Saeedeh Sadat Hosseini ◽  
Salman Ahmadian ◽  
Sonia Boughattas ◽  
Gilda Eslami ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cutaneous Leishmaniasis ◽  
Molecular Characterization ◽  
Significance Level ◽  
Detection And Identification ◽  
Pcr Rflp ◽  
Leishmania Spp ◽  
Sensitive Isolate ◽  
The One

Background: Regarding the antimonial-resistant of Leishmania spp., understanding of related mechanism is neces­sary. One of the most important involved molecules is aquaglyceropin1 (AQP1). The aim of this study was molecu­lar analysis of AQP1 gene from antimonial-resistant clinical isolates and its expression. Methods: Overall, 150 patients with cutaneous leishmaniasis referring to the reference laboratories of Yazd and Varzaneh,, located 105km southeast of Isfahan and 240km away from Yazd, were assessed from Jun 2015 to Dec 2017. After sampling, staining was done and evaluated for Leishman by microscope. Samples were collected in RNAlater solution for gene expression analysis in non-healing isolates. DNA extraction was performed from each slide with Leishman body. All patients with L. major isolates detected by ITS1-PCR-RFLP were followed for find­ing the resistant isolates, consequence of molecular characterization of AQP1 using PCR-RFLP. Gene expression of AQP1 from all resistant isolates was assessed in comparison with the one in a sensitive isolate. Statistical analysis was done using SPSS. The significance level was considered ≤0.05. Results: Five isolates were detected as antimonial resistant. Molecular detection and identification were appeared that all were L. major. The molecular characterization of AQP1 showed G562A mutation. Gene expression of AQP1 in resistant isolates showed 1.67 fold higher than the sensitive isolate. Conclusion: We reported a new point mutation of G562A in AQP1 gene involved in molecular mechanism in re­sistant isolates.

scholarly journals Canine visceral leishmaniasis: perception, prevalence, and spatial distribution in municipality of Nossa Senhora do Livramento, Mato Grosso, Brazil

2020 ◽  
Vol 29 (2) ◽  
Author(s):  
Matheus Roberto Carvalho ◽  
Álvaro Felipe de Lima Ruy Dias ◽  
Arleana do Bom Parto Ferreira de Almeida ◽  
Mário Ribeiro Alves ◽  
Adilson Santos Paes ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Visceral Leishmaniasis ◽  
Rapid Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Visceral Leishmaniasis ◽  
Mato Grosso ◽  
Rural And Urban ◽  
Spatially Distributed ◽  
Control And Prevention ◽  
Leishmania Spp

Abstract The midwest region of Brazil has witnessed an increase in the number of cases of human (HVL) and canine visceral leishmaniasis (CVL). The aim of the present study was to evaluate the population’s perception of these diseases, factors associated with CVL, its spatial distribution, and the prevalence of anti-Leishmania spp. in 385 dogs, determined using a commercially available immunochromatographic rapid test and confirmed using enzyme-linked immunosorbent assay (ELISA). Of the 385 samples, 54 were positive for CVL and confirmed by ELISA, corresponding to a prevalence of 14%. Knowledge of signs of CVL by tutors (p = 0.038); previous occurrence of CVL in the vicinity (p = 0.022); symptomatic dog(s) (p = 0.014), splenomegaly (p = 0.055), and ear ulcer(s) (p = 0.059) were significantly associated with CVL. The results revealed a significant prevalence of CVL spatially distributed in rural and urban contexts. The association between environmentally related variables and perception and the occurrence of CVL underscores the importance of implementing control and prevention strategies primarily focused on environmental management and health education activities.

Comparison of serological and molecular tests to investigate Leishmania spp. infections in stray dogs from an area of intense visceral leishmaniasis transmission in Brazil

2021 ◽  
Vol 30 (3) ◽  
Author(s):  
Anna Maria da Cruz Ferreira Evaristo ◽  
Andreina de Carvalho Araujo ◽  
Andréa Pereira da Costa ◽  
Kamila Gaudêncio da Silva Sales ◽  
José Alexandre Menezes da Silva ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Quantitative Polymerase Chain Reaction ◽  
Skin Lesions ◽  
Leishmania Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Tests ◽  
Stray Dogs ◽  
Transmission Cycle ◽  
Molecular Assays ◽  
Leishmania Spp

Abstract The aim of this study was to investigate the level of exposure to Leishmania infection in stray dogs in an area of intense visceral leishmaniasis transmission in the state of Pernambuco, Brazil. Blood samples from 178 dogs were analyzed using serological and molecular assays: rapid immunochromatographic test (ICT), enzyme-linked immunosorbent assay (ELISA), immunofluorescence antibody test (IFAT), and conventional and quantitative polymerase chain reaction (cPCR and qPCR). Positivity values obtained with serological tests were 71.4% (127/178), 70.2% (125/178), and 50.6% (90/178) using ICT, ELISA, and IFAT, respectively, with 38.8% (69/178) of the dogs were simultaneously positive for all three tests. The positivity values obtained with cPCR and qPCR were 20.2% (36/178) and 38.8% (69/178), respectively, with 11.8% (21/178) testing positive in both molecular assays. Overall, 87.1% (155/178) were positive for anti-Leishmania spp. antibodies and/or Leishmania spp. DNA. Positivity to one or more tests was statistically associated with lymphadenomegaly, skin lesions, lymphocytosis, anemia and hyperproteinemia. The results of this study revealed a high level of exposure to Leishmania in stray dogs in an area of intense human visceral leishmaniasis transmission, suggesting that dogs play a role as reservoirs in the transmission cycle of this zoonosis.

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