Human adipose tissue-derived stromal cells in combination with exogenous stimuli facilitate three-dimensional network formation of human endothelial cells derived from various sources

2018 ◽  
Vol 106 ◽  
pp. 28-36 ◽  
Author(s):  
Dominique Manikowski ◽  
Birgit Andrée ◽  
Esther Samper ◽  
Clémence Saint-Marc ◽  
Ruth Olmer ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Network Formation ◽  
Three Dimensional ◽  
Human Adipose Tissue ◽  
Human Endothelial Cells ◽  
Dimensional Network

scholarly journals Mitigating the non-specific uptake of immunomagnetic microparticles enables the extraction of endothelium from human fat

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Jeremy A. Antonyshyn ◽  
Vienna Mazzoli ◽  
Meghan J. McFadden ◽  
Anthony O. Gramolini ◽  
Stefan O. P. Hofer ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Reliable Method ◽  
Vascular Tissue ◽  
Human Adipose Tissue ◽  
Vascular Tissue Engineering ◽  
Microvascular Endothelial Cells ◽  
Specific Uptake

AbstractEndothelial cells are among the fundamental building blocks for vascular tissue engineering. However, a clinically viable source of endothelium has continued to elude the field. Here, we demonstrate the feasibility of sourcing autologous endothelium from human fat – an abundant and uniquely dispensable tissue that can be readily harvested with minimally invasive procedures. We investigate the challenges underlying the overgrowth of human adipose tissue-derived microvascular endothelial cells by stromal cells to facilitate the development of a reliable method for their acquisition. Magnet-assisted cell sorting strategies are established to mitigate the non-specific uptake of immunomagnetic microparticles, enabling the enrichment of endothelial cells to purities that prevent their overgrowth by stromal cells. This work delineates a reliable method for acquiring human adipose tissue-derived microvascular endothelial cells in large quantities with high purities that can be readily applied in future vascular tissue engineering applications.

scholarly journals Matrix composition regulates three-dimensional network formation by endothelial cells and mesenchymal stem cells in collagen/fibrin materials

Angiogenesis ◽  
2012 ◽  
Vol 15 (2) ◽  
pp. 253-264 ◽  
Author(s):  
Rameshwar R. Rao ◽  
Alexis W. Peterson ◽  
Jacob Ceccarelli ◽  
Andrew J. Putnam ◽  
Jan P. Stegemann
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Mesenchymal Stem Cells ◽  
Network Formation ◽  
Three Dimensional ◽  
Matrix Composition ◽  
Dimensional Network

scholarly journals Human adipose tissue-derived stromal cells act as functional pericytes in mice and suppress high-glucose-induced proinflammatory activation of bovine retinal endothelial cells

Diabetologia ◽  
2018 ◽  
Vol 61 (11) ◽  
pp. 2371-2385 ◽  
Author(s):  
Ghazaleh Hajmousa ◽  
Ewa Przybyt ◽  
Frederick Pfister ◽  
Genaro A. Paredes-Juarez ◽  
Kondaiah Moganti ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
High Glucose ◽  
Human Adipose Tissue ◽  
Retinal Endothelial Cells

Insulinlike growth factor action and production in adipocytes and endothelial cells from human adipose tissue

Diabetes ◽  
1989 ◽  
Vol 38 (6) ◽  
pp. 710-717 ◽  
Author(s):  
P. A. Kern ◽  
M. E. Svoboda ◽  
R. H. Eckel ◽  
J. J. Van Wyk
Keyword(s):  
Endothelial Cells ◽  
Adipose Tissue ◽  
Growth Factor ◽  
Human Adipose Tissue ◽  
Insulinlike Growth Factor

Molecular mechanisms underlying human adipose tissue-derived stromal cells differentiation into a hepatocyte-like phenotype

2010 ◽  
Vol 42 (12) ◽  
pp. 895-901 ◽  
Author(s):  
Nathalie Saulnier ◽  
Anna Chiara Piscaglia ◽  
Maria Ausiliatrice Puglisi ◽  
Marta Barba ◽  
Vincenzo Arena ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Molecular Mechanisms ◽  
Human Adipose Tissue
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