Protein disulfide isomerase is a central regulator of NADPH oxidase activity

2006 ◽  
Vol 45 (3) ◽  
pp. e71
Author(s):  
Mariano Janiszewski ◽  
Katrin Schröder ◽  
Rudi Busse ◽  
Francisco Laurindo ◽  
Ralf P. Brandes
Keyword(s):  
Nadph Oxidase ◽  
Oxidase Activity ◽  
Protein Disulfide Isomerase ◽  
Disulfide Isomerase ◽  
Nadph Oxidase Activity ◽  
Protein Disulfide

Abstract 776: Protein Disulfide Isomerase Is Involved In Redox Regulation Of Nitric Oxide Output During Laminar Shear Stress In Endothelial Cells

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Denise C Fernandes ◽  
Celio X Santos ◽  
Hanjoong Jo ◽  
Francisco R Laurindo
Keyword(s):  
Endothelial Cells ◽  
Nadph Oxidase ◽  
Oxidase Activity ◽  
Membrane Fraction ◽  
Protein Disulfide Isomerase ◽  
Disulfide Isomerase ◽  
Nadph Oxidase Activity ◽  
Caveolin 1 ◽  
Protein Disulfide ◽  
Laminar Shear

While anti-atherogenic effects of sustained laminar shear (LS) involve NO release from eNOS, increases in LS trigger transient superoxide production via NADPH oxidase. Recently, we showed that NADPH oxidase undergoes thiol-dependent regulation by the thioredoxin superfamily chaperone Protein Disulfide Isomerase (PDI). PDI is known to promote NO internalization via trans-nitrosation reactions. We hypothesized that PDI-dependent support of NADPH oxidase activity affects NO output during sustained LS. Cultured rabbit aortic endothelial cells (RAEC) submitted to LS (15 dynes/cm 2 ) in a cone-plate system for 18h exhibited (vs. static controls): Decreased (~50%) superoxide production (HPLC analysis of DHE oxidation); Decreased (~20%) NADPH-triggered hydrogen peroxide production in membrane fraction (Amplex Red assay); Decreased mRNA expression of Nox1 (67%) and Nox4 (45%) (real-time QPCR); Increased eNOS expression (~50%, western blot) and nitrite levels in culture medium (Δ = 7.1±2.5[SD] μM, NO Analyzer and Griess reaction); Decrease in total and membrane fraction PDI protein expression (~20%) without changes in membrane fraction/total ratio of PDI. RAEC were transfected with c-myc -tagged plasmid coding for wild-type (WT) PDI or PDI mutated in 4 thioredoxin-motif cysteine residues. Forced expression (2-fold) of mutated but not WT PDI led to increase in nitrite output after LS (18h) (Δmutated = 17.2±3.3 μM vs. ΔWT = 7.0±1.9 μM, n=3, p<0.02). Confocal microscopy indicated similar subcellular localization between WT and mutated PDI. PDI co-imunoprecipitated with p22phox NADPH oxidase subunit, but not with eNOS or caveolin-1, either in static condition or after LS. Fractionation studies in sucrose gradients showed that PDI is distributed throughout several fractions in static conditions, including caveolin-1-enriched fractions, but migrates to higher-density fractions, not containing caveolin-1, during sustained LS. These results suggest that PDI is involved in regulation of NO output during LS via its effects on NADPH oxidase activity.

Novel Role of Protein Disulfide Isomerase in the Regulation of NADPH Oxidase Activity: Pathophysiological Implications in Vascular Diseases

2008 ◽  
Vol 10 (6) ◽  
pp. 1101-1114 ◽  
Author(s):  
Francisco R.M. Laurindo ◽  
Denise C. Fernandes ◽  
Angélica M. Amanso ◽  
Lucia R. Lopes ◽  
Célio X.C. Santos
Keyword(s):  
Nadph Oxidase ◽  
Oxidase Activity ◽  
Protein Disulfide Isomerase ◽  
Vascular Diseases ◽  
Disulfide Isomerase ◽  
Nadph Oxidase Activity ◽  
Protein Disulfide

scholarly journals Protein disulfide isomerase is a central regulator of NADPH oxidase activity

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Ralf P. Brandes ◽  
Rudi Busse ◽  
Francisco R Laurindo ◽  
Mariano Janiszewski
Keyword(s):  
Nadph Oxidase ◽  
Oxidase Activity ◽  
Protein Disulfide Isomerase ◽  
Disulfide Isomerase ◽  
Nadph Oxidase Activity ◽  
Protein Disulfide

Protein Disulfide Isomerase (PDI) Regulation of NADPH Oxidase Activity: Effects on Angiotensin II Redox Signaling in Hypertension

2010 ◽  
Vol 49 ◽  
pp. S16
Author(s):  
Livia de Lucca Camargo ◽  
Aline Cristianne Depoli Androwiki ◽  
Graziela Scalianti Ceravolo ◽  
Alexandre Denadai-Souza ◽  
Marcelo Nicolas Muscara ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Nadph Oxidase ◽  
Oxidase Activity ◽  
Protein Disulfide Isomerase ◽  
Redox Signaling ◽  
Disulfide Isomerase ◽  
Nadph Oxidase Activity ◽  
Protein Disulfide

Protein disulfide isomerase (PDI) associates with NADPH oxidase and is required for phagocytosis ofLeishmania chagasipromastigotes by macrophages

2009 ◽  
Vol 86 (4) ◽  
pp. 989-998 ◽  
Author(s):  
Célio X. C. Santos ◽  
Beatriz S. Stolf ◽  
Paulo V. A. Takemoto ◽  
Angélica M. Amanso ◽  
Lucia R. Lopes ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Protein Disulfide Isomerase ◽  
Disulfide Isomerase ◽  
Protein Disulfide

scholarly journals Subverted regulation of Nox1 NADPH oxidase-dependent oxidant generation by protein disulfide isomerase A1 in colon carcinoma cells with overactivated KRas

2019 ◽  
Vol 10 (2) ◽  
Author(s):  
Tiphany Coralie De Bessa ◽  
Alessandra Pagano ◽  
Ana Iochabel Soares Moretti ◽  
Percillia Victoria Santos Oliveira ◽  
Samir Andrade Mendonça ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Colon Carcinoma ◽  
Protein Disulfide Isomerase ◽  
Carcinoma Cells ◽  
Disulfide Isomerase ◽  
Colon Carcinoma Cells ◽  
Protein Disulfide

scholarly journals Protein disulfide isomerase expression increases in resistance arteries during hypertension development. Effects on Nox1 NADPH oxidase signaling

2015 ◽  
Vol 3 ◽  
Author(s):  
Aline C. D. Androwiki ◽  
Lívia de Lucca Camargo ◽  
Simone Sartoretto ◽  
Gisele K. Couto ◽  
Izabela M. R. Ribeiro ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Protein Disulfide Isomerase ◽  
Resistance Arteries ◽  
Disulfide Isomerase ◽  
Hypertension Development ◽  
Protein Disulfide
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