In vivo stem cell seeding of matrix material as a new tool in heart valve tissue engineering

2006 ◽  
Vol 45 (3) ◽  
pp. e31
Author(s):  
Ilse Vranken ◽  
Geofrey De Visscher ◽  
An Lebacq ◽  
Willem Flameng
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Heart Valve ◽  
Matrix Material ◽  
Cell Seeding ◽  
Valve Tissue ◽  
Heart Valve Tissue ◽  
Heart Valve Tissue Engineering

scholarly journals In vivo cellularization of a cross-linked matrix by intraperitoneal implantation: a new tool in heart valve tissue engineering

2007 ◽  
Vol 28 (11) ◽  
pp. 1389-1396 ◽  
Author(s):  
G. De Visscher ◽  
I. Vranken ◽  
A. Lebacq ◽  
C. Van Kerrebroeck ◽  
J. Ganame ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Heart Valve ◽  
Valve Tissue ◽  
Heart Valve Tissue ◽  
Heart Valve Tissue Engineering

scholarly journals Assembly and Testing of Stem Cell-Seeded Layered Collagen Constructs for Heart Valve Tissue Engineering

2011 ◽  
Vol 17 (1-2) ◽  
pp. 25-36 ◽  
Author(s):  
Mary E. Tedder ◽  
Agneta Simionescu ◽  
Joseph Chen ◽  
Jun Liao ◽  
Dan T. Simionescu
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Heart Valve ◽  
Valve Tissue ◽  
Heart Valve Tissue ◽  
Heart Valve Tissue Engineering

scholarly journals Inflammatory and regenerative processes in bioresorbable synthetic pulmonary valves up to 2 years in sheep: Spatiotemporal insights augmented by Raman microspectroscopy

2021 ◽  
Author(s):  
Bente J de Kort ◽  
Julia Marzi ◽  
Eva Brauchle ◽  
Arturo M. Lichauco ◽  
Hannah S Bauer ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Heart Valve ◽  
Raman Microspectroscopy ◽  
Regenerative Processes ◽  
Valve Tissue ◽  
Heart Valve Tissue ◽  
Heart Valve Tissue Engineering

In situ heart valve tissue engineering is an emerging approach in which resorbable, off-the-shelf porous polymeric scaffolds containing leaflets and conduit are used to induce endogenous heart valve restoration. Such scaffolds are designed to recruit endogenous cells in vivo, which subsequently resorb polymer and produce and remodel new valvular tissue in situ. Recently, preclinical studies using electrospun supramolecular elastomeric valvular grafts have shown that this approach enables in situ regeneration of pulmonary valves with long-term functionality in vivo. However, the evolution and mechanisms of inflammation, polymer absorption and tissue regeneration are largely unknown, and adverse valve remodeling and intra- and inter-valvular variability have been reported. Therefore, the goal of the present study was to gain a mechanistic understanding of the in vivo regenerative processes by combining routine histology and immunohistochemistry, using a comprehensive sheep-specific antibody panel, with Raman microspectroscopy for the spatiotemporal analysis of in situ tissue-engineered pulmonary valves with follow-up to 24 months from a previous preclinical study in sheep. The analyses revealed a strong spatial heterogeneity in the influx of inflammatory cells, graft resorption, and foreign body giant cells. Collagen maturation occurred predominantly between 6 and 12 months after implantation, which was accompanied by a progressive switch to a more quiescent phenotype of infiltrating cells with properties of valvular interstitial cells. Variability among specimens in the extent of tissue remodeling was observed for follow-up times after 6 months. Taken together, these findings advance the understanding of key events and mechanisms in material-driven in situ heart valve tissue engineering.

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