The antiatherogenic and antiinflammatory effect of HDL-associated lysosphingolipids operates via Akt à NF-kappaB signalling pathways in human vascular endothelial cells

2006 ◽  
Vol 45 (3) ◽  
pp. e22-e23
Author(s):  
Annette Schmidt ◽  
Sven Geigenmüller ◽  
Eckhart Buddecke
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Signalling Pathways ◽  
Vascular Endothelial ◽  
Antiinflammatory Effect ◽  
Nf Kappab

Tu-W19:3 Proinflammatory cytokine mediated T-PA suppression in vascular endothelial cells is NF-KAPPAB and P38 dependent

2006 ◽  
Vol 7 (3) ◽  
pp. 159
Author(s):  
P. Larsson ◽  
E. Ulfhammer ◽  
L. Karlsson ◽  
W. Ridderstrle ◽  
T. Hrafnkelsdottir ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Proinflammatory Cytokine ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Nf Kappab

scholarly journals Involvement of interleukin-8, vascular endothelial growth factor, and basic fibroblast growth factor in tumor necrosis factor alpha-dependent angiogenesis.

1997 ◽  
Vol 17 (7) ◽  
pp. 4015-4023 ◽  
Author(s):  
S Yoshida ◽  
M Ono ◽  
T Shono ◽  
H Izumi ◽  
T Ishibashi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Vascular Endothelial Cells ◽  
Tnf Alpha ◽  
Vascular Endothelial ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Nf Kappab ◽  
Endothelial Growth Factor

Tumor necrosis factor alpha (TNF-alpha) is a macrophage/monocyte-derived polypeptide which modulates the expression of various genes in vascular endothelial cells and induces angiogenesis. However, the underlying mechanism by which TNF-alpha mediates angiogenesis is not completely understood. In this study, we assessed whether TNF-alpha-induced angiogenesis is mediated through TNF-alpha itself or indirectly through other TNF-alpha-induced angiogenesis-promoting factors. Cellular mRNA levels of interleukin-8 (IL-8), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and their receptors were increased after the treatment of human microvascular endothelial cells with TNF-alpha (100 U/ml). TNF-alpha-dependent tubular morphogenesis in vascular endothelial cells was inhibited by the administration of anti-IL-8, anti-VEGF, and anti-bFGF antibodies, and coadministration of all three antibodies almost completely abrogated tubular formation. Moreover, treatment with Sp1, NF-kappaB, and c-Jun antisense oligonucleotides inhibited TNF-alpha-dependent tubular morphogenesis by microvascular endothelial cells. Administration of a NF-kappaB antisense oligonucleotide almost completely inhibited TNF-alpha-dependent IL-8 production and partially abrogated TNF-alpha-dependent VEGF production, and an Sp1 antisense sequence partially inhibited TNF-alpha-dependent production of VEGF. A c-Jun antisense oligonucleotide significantly inhibited TNF-alpha-dependent bFGF production but did not affect the production of IL-8 and VEGF. Administration of an anti-IL-8 or anti-VEGF antibody also blocked TNF-alpha-induced neovascularization in the rabbit cornea in vivo. Thus, angiogenesis by TNF-alpha appears to be modulated through various angiogenic factors, both in vitro and in vivo, and this pathway is controlled through paracrine and/or autocrine mechanisms.

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